Applications

In this application note, an RMS1000 Confocal Microscope is used to image a tissue section of mouse intestine using two-photon excited fluorescence and second harmonic generation microscopy.

Single-wall carbon nanotubes (SWCNTs) have unique electrical, thermal, mechanical, and optical properties which make them attractive for a wide variety of applications; ranging from drug delivery to battery electrodes. In this application note the FLS1000 Photoluminescence Spectrometer equipped with an InGaAs NIR camera is used to identify the chiral indexes present in a SWCNT sample using excitation emission matrix spectroscopy.

Raman spectroscopy is an analytical technique which can be used both quantitatively and qualitatively. This application note details the quantitative use of Raman spectroscopy to determine ethanol content in samples of whisky. Qualitatively, Raman spectroscopy can also be used for whisky analysis to ensure it does not contain methanol, a toxic alcohol which can be fraudulently used in alcohol sales to boost profits.

Molecular beacon probes are a sequence of nucleotides (the building blocks of DNA and RNA) that can be used to fluorescently detect the presence of a specific sequence of DNA or RNA. With real-world examples as PCR quantification, in vivo RNA detection, pathogen detection and viral load quantification. The use of molecular beacons, coupled with a sensitive spectrofluorometer facilitates the measurement of extremely low concentrations of DNA or RNA. In this application note, nanomolar concentrations of cDNA were quantified using a molecular beacon while controlling the temperature of incubation and measuring the sample emission with an Edinburgh Instruments FS5 Spectrofluorometer.

Hand sanitiser needs to >60% ethanol to be effective at killing microbes on your hands. This Application Note details how Raman spectroscopy can be used to create ethanol calibration curves providing a rapid method for ensuring hand sanitisers meet the 60% requirement.

Quantum yield is a fundamental photophysical parameter that describes a sample's fluorescence efficiency, and it can be measured optically via the absolute method and relative method. 2-Aminopyridine (2AMP) in sulfuric acid (H2SO4) has been previously used as a quantum yield reference standard in the UV-visible range. However, the 2AMP quantum yield values are now decades old. In this application note, we present a reinvestigation and revaluation of the quantum yield of 2AMP in 1M H2SO4, using quinine bisulphate (QBS) in 1M H2SO4 as the reference standard with an Edinburgh Instruments FS5 Spectrofluorometer.

Heterometallic metal halide hybrids hold great promise due to the potential for synergistic photo-physical properties depending on the choice of the constituent metal centres. In this application note time-resolved emission spectroscopy (TRES) with the FLS1000 Photoluminescence Spectrometer is used to identify the emission pathways in the 0D metal halide hybrid, Tris SbMnCl.

Efficient extraction of charge carriers is critical for the creation of high-efficiency solar cells. Photoluminescence is proportional to the number of charge carriers in the perovskite and therefore sensitive to charge transfer into adjacent layers which makes photoluminescence based techniques invaluable for investigating the performance of new extraction layers. In this application note, the extraction efficiency of a VACNT hole extraction layer is investigated using PL spectroscopy with an Edinburgh Instruments FLS1000 Photoluminescence Spectrometer.

The terahertz spectral region, which falls between the microwave and infrared ranges, is one of the most promising regions of the electromagnetic spectrum for research and industry but it is currently underutilised in these areas. One of the most versatile sources for spectroscopy and imaging research in this region, is the Molecular Terahertz Laser. Find out how these lasers work and their applications in industrial and scientific imaging, materials analysis and biomedical research in this application note.

How can Raman microscopy help the battle to quit smoking? Read this latest application note to find out how the RMS1000 can aid pharmaceutical laboratories quality control nicotine patches via 3D Raman mapping.