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What is Fluorescence Quenching?

Fluorescence quenching is the decrease in fluorescence intensity of an emitter due to interaction with a quencher.

Constant Fraction Determination

Time-Correlated Single-Photon Counting receives non-uniform pulses from the excitation source and the sample, so how do we ensure the timing electronics precisely record arrival time? Discover how constant fraction discrimination enables accurate timing despite pulse fluctuations and system noise.

Determining Fluorescence Lifetimes

Why and how we fit components to recorded TCSPC data. Using a variety of examples, we show how different systems need to be explored in different ways from the same TCSPC system.

Infrared or Raman Spectroscopy?

IR and Raman spectroscopy are two complimentary vibrational spectroscopy techniques. How to choose which one to use? Our Spectral School article explains how to tell if your samples are better suited to Raman or IR spectroscopy and why.

What is FLIM?

An introduction to Fluorescence Lifetime Imaging Microscopy (FLIM) and how it works.

Time Correlated Single Photon Counting - Why use TCSPC for Fluorescence Lifetime Measurements?

Time Correlated Single-Photon Counting (TCSPC) is the method of choice for fluorescence lifetime measurements. This blog post follows an introduction to Time Correlated Single Photon Counting, which answers the question; What is TCSPC?

What is Multichannel Scaling (MSC)

An introduction to Multi-Channel Scaling which is the ideal technique for identifying phosphorescent lifetimes.

Illumination Techniques in Optical Microscopy

In this Spectral School Tutorial, we introduce some of the most common optical microscopy terms and techniques, including upright and inverted microscopes, reflected and transmitted illumination modes, and brightfield and darkfield contrast mechanisms, and discuss their applicability with respect to different research areas.

TCSPC - What is Time-Correlated Single Photon Counting?

A first introduction to the popular method of Time-Correlated Single Photon Counting which may be used to separate multiple fluorescence lifetimes from a single sample.

Photoluminescence Spectroscopy Poster Presentation Series – TADF Emitter Characterisation

This poster presentation video illustrates how to fully characterise the photophysics of a Thermally Activated Delayed Fluorescence (TADF) material for OLED applications using an FS5 Spectrofluorometer. Spectral and time-resolved photoluminescence, quantum yield, and temperature dependence are all studied in a compact FS5 instrument.