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Fluoracle Software

Fluoracle Software

Fluoracle controls all FS5, Mini-tau and FLS1000 series of steady state and time-resolved spectrometers and features a straightforward design concept:

“to focus on all modern photoluminescence spectroscopy applications whilst providing a user-friendly interface with ‘ready to publish’ outputs”

Product Description

Fluoracle® is an exceptional software package specifically written to handle data and instrumentation in fluorescence spectroscopy.

Fluoracle is the successor of the proven F980 software from our FLS980 product line and it is this that provides the backbone to Fluoracle.

Whether you select a basic spectral scanning version of one of our spectrometer systems, or you go for an advanced version that includes TCSPC lifetime measurements, or integrating sphere measurements, the software will provide all instrument options automatically, from data acquisition to analysis and presentation.

Fluorescence Lifetime Imaging Microscopy (FLIM) acquisition and analysis are included in Fluoracle with a MicroPL upgrade.

Technical Specifications

Software Functionality for Steady State Spectroscopy
Measurement Modes Control Features Data Manipulation and Display
Signal rates Wavelength selection (λEX & λEM) Arithmetic ( + , – , × , / , append )
Excitation spectra Grating selection Scaling / multiplication factor
Emission spectra Spectral bandwidths Normalise
Anisotropy spectra Integration time per data point (1 ms – 200 s) Baseline subtraction
Kinetic measurements Sample selection (multi-position sample wells) Zoom / Crop range
Synchronous spectra Programmed excitation shutter Smooth
Corrected spectra Programmed attenuator 2D, 3D, Contour and text
Temperature resolved spectra map Source and detector selection Grid ON / OFF
Synchronous spectra map Online spectral data correction Differentiation / Integration
Excitation-emission map Post acquisition spectral correction Peak search and display
Sample temperature monitoring Polariser selection and orientation Correction
Reflection measurements X-Y Sample stage control Anisotropy (G factor corrected)
Absorption measurements Sample temperature control Logarithmic / linear scales
Absorptance Sample selection (multiple cuvette holders) Comprehensive measurement and file properties for record keeping
Reflectance Temperature control of sample holders Data manipulation and ASCII / CSV output
Batch measurements Detector gating for phosphorescence spectra Cursor locations
Absolute quantum yield measurements Cryostat control Join, split and extract frames
Multiple sample position and well-plate measurements Microscope stage control Spectral correction wizard
Service scans Plate reader control Absolute quantum yield wizard
Titrator control Chromaticity calculation and display wizard

Software Functionality for Time-Resolved Photoluminescence Measurements
Measurement modes Control Features Analysis Features
Signal rates Wavelength selection for excitation and emission monochromators Full data reconvolution using a non-linear least square fitting routine:
Manual lifetime measurement Sample selection (multi-position sample holders) Exponential reconvolution or simple tail fit
Multiple lifetime measurement Detector selection (up to 3) 1-4 independent exponential decay times, fixed or as free fit parameters
Instrument Response Measurement nF920 flashlamp voltage, frequency control and gas pressure monitor Shift parameters, fixed or as a free fit parameter
Time resolved excitation spectra External Laser Triggering Background fit, fixed or as a free fit parameter
Time resolved emission spectra Programmable iris attenuator Chi-squared goodness-of-fit test
Fluorescence anisotropy Multiple sources Weighted residuals, Durbin-Watson parameter
Temperature controlled lifetime measurements Polariser selection and orientation Autocorrelation function
Sample temperature monitoring Cryostat control Anisotropy calculation
Multiple sample position and well-plate measurements Measurement to peak counts or preset time Time resolved spectra
Forward or Reverse mode
CFD settings on START and STOP: threshold, zero crossing and divider
Channel Selection: 512 – 8192 channels (TCSPC) and 500 – 8000 channels (MCS)
Time Range Selection: 2.5 ns – 50 µs (TCSPC) and 5 ms – 1000 s (MCS)