Steady State Fluorescence
Steady-State Fluorescence Spectroscopy investigates the long-term average fluorescence of a sample when irradiated with UV, Visible or near-IR Light.
Edinburgh Instruments offers a range of research-grade and analytical Steady State Spectrofluorometers. These vary in a number of ways but can be compact, benchtop or modular, and fully customisable for any type of fluorescence measurements that will meet the most demanding research requirements.
- Absolute Quantum Yield Measurements
- Steady State Singlet Oxygen Emission
- Water Quality Assessment
- Phosphor Powders Composition Analysis
- Fluorescence Upconversion
The fluorescence spectrum is a plot of fluorescence intensity vs. the registered wavelength (energy and frequency) at one excitation wavelength. These fluorescence intensity measurements (spectral measurements) allow the determination of the presence of fluorophores and their concentrations.
Spectral measurements are made using a technique called Single Photon Counting (SPC). All instruments manufactured by Edinburgh Instruments detect photons by using suitable Photomultiplier Tubes (PMTs) detectors. Using PMTs and single photon counting allows for the ultimate in accuracy and sensitivity in the acquisition of data.
Steady State measurement examples include:
- Excitation and Emission Scans
- Synchronous Scans & Maps
- Steady State Fluorescence Anisotropy
- Excitation-Emission Maps (EEM)
- Kinetic Measurements
- Temperature Maps
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