Steady State Fluorescence

Steady-State Fluorescence Spectroscopy investigates the long-term average fluorescence of a sample when irradiated with UV, Visible or near-IR Light.

Edinburgh Instruments offers a range of research-grade and analytical Steady State Spectrofluorometers. These vary in a number of ways but can be compact, benchtop or modular, and fully customisable for any type of fluorescence measurements that will meet the most demanding research requirements.

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FLS1000 Spectrometer

FS5 Spectrofluorometer

Absolute Quantum Yield Measurements
Steady State Singlet Oxygen Emission
Water Quality Assessment
Phosphor Powders Composition Analysis
Fluorescence Upconversion

Author	Year	Title	Journal	Vol.	Pages	Instrument
Anna Gakamsky et al.	2017	Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular Level	Scientific Reports	7	40375	FLS980 Spectrometer	Pulsed Diode Lasers – VIS/NIR	Pulsed LEDs – UV/VIS	Advanced FAST Software
Cédric Mongin et al.	2016	Direct observation of triplet energy transfer from semiconductor nanocrystals	Science	351	369-372	LP920 Upgrades	FLS920 Upgrades	Mini-tau	Pulsed Diode Lasers – VIS/NIR
Eli Zysman-Colman et al.	2016	Solution-Processable Silicon Phthalocyanines in Electroluminescent and Photovoltaic Devices	ACS Applied Materials & Interfaces	8	9247-9253	FLS980 Spectrometer	FLS980 Upgrades
M. A. Shipman, K. J. Ramhita and B. A. Blight	2016	Sensing a Bacillis anthracis biomarker with well-known OLED emitter EuTta3Phen	Journal of Materials Chemistry B			FS5 Spectrofluorometer
Xixi Qin et al.	2016	Hybrid coordination-network-engineering for bridging cascaded channels to activate long persistent phosphorescence in the second biological window	Nature Scientific Reports	6	20275	FLS920 Upgrades
De-Chao Yu et al.	2015	Multi-photon quantum cutting in Gd2O2S:Tm3+ to enhance the photo-response of solar cells	Light: Science & Applications	4	e344	FLS920 Upgrades
Like Huanga et al.	2016	Efficient planar perovskite solar cells without a high temperature processed titanium dioxide electron transport layer	Solar Energy Materials and Solar Cells	149	1-8	FLS920 Upgrades
Yanyan Li et al.	2014	A Single-Component White-Emitting CaSr2Al2O6:Ce3+, Li+, Mn2+ Phosphor via Energy Transfer	Inorganic Chemistry	53	7668-7675	FLS920 Upgrades	LP920 Upgrades
Chen Liao et. al	2015	Bright white-light emission from Ag/SiO2/CdS-ZnS core/shell/shell plasmon couplers	Nanoscale			FLS920 Upgrades
Ahmad S. Sediq et al.	2016	No Touching! Abrasion of Adsorbed Protein Is the Root Cause of Subvisible Particle Formation During Stirring	Journal of Pharmaceutical Sciences			FLS920 Upgrades
Yuren Wang, Haiching Ma	2015	Protein kinase profiling assays: a technology review	Drug Discovery Today: Technologies	18	1-8	FLS920 Upgrades
Christos Pliotas et al.	2015	The role of lipids in mechanosensation	Nature Structural & Molecular Biology	22	991-998	FLS920 Upgrades
Choi, M. K. et al.	2015	Wearable red-green-blue quantum dot light-emitting diode array using high-resolution intaglio transfer printing	Nature Communications	6	7149	FLS980 Spectrometer	Pulsed Diode Lasers – VIS/NIR
Bilel Louahem M’Sabah et al.	2015	Amplification of light collection in solid-state dye-sensitized solar cells via the antenna effect through supramolecular assembly	Physical Chemistry Chemical Physics	17	9910-9918	FLS980 Spectrometer
Ruichan Lva et al.	2015	An imaging-guided platform for synergistic photodynamic/photothermal/chemo-therapy with pH/temperature-responsive drug release	Biomaterials	63	115-127	FLS980 Spectrometer
Thomas J. Macdonald et al.	2014	Cation exchange of aqueous CuInS2 quantum dots	CrystEngComm	16	9455-9460	FLS980 Spectrometer
Renato Grillo et al.	2015	Chitosan nanoparticles loaded the herbicide paraquat: The influence of the aquatic humic substances on the colloidal stability and toxicity	Journal of Hazardous Materials	286	562-572	FLS980 Spectrometer

The fluorescence spectrum is a plot of fluorescence intensity vs. the registered wavelength (energy and frequency) at one excitation wavelength. These fluorescence intensity measurements (spectral measurements) allow the determination of the presence of fluorophores and their concentrations.

Spectral measurements are made using a technique called Single Photon Counting (SPC). All instruments manufactured by Edinburgh Instruments detect photons by using suitable Photomultiplier Tubes (PMTs) detectors. Using PMTs and single photon counting allows for the ultimate in accuracy and sensitivity in the acquisition of data.

Steady State measurement examples include:

Excitation and Emission Scans
Synchronous Scans & Maps
Steady State Fluorescence Anisotropy
Excitation-Emission Maps (EEM)
Kinetic Measurements
Temperature Maps

In Techniques

Steady State Fluorescence

FLS1000 Spectrometer

FS5 Spectrofluorometer