Steady State Fluorescence | Fluorescence Spectroscopy Technique | Edinburgh Instruments
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Steady State Fluorescence

Steady State Fluorescence Spectroscopy investigates the long-term average fluorescence of a sample when irradiated with UV, Visible or near-IR Light.

Edinburgh Instruments offers a range of research grade and analytical steady state spectrofluorometers. These vary in a number of ways but can be compact, benchtop or modular, and fully customisable for any type of fluorescence measurements that will meet the most demanding research requirements. For further information or to discuss your requirements, please contact us.

Description

The fluorescence spectrum is a plot of fluorescence intensity vs. the registered wavelength (energy and frequency) at one excitation wavelength. These fluorescence intensity measurements (spectral measurements) allow the determination of the presence of fluorophores and their concentrations.

Spectral measurements are made using a technique called Single Photon Counting (SPC). All instruments manufactured by Edinburgh Instruments detect photons by using suitable Photomultiplier Tubes (PMTs) detectors. Using PMTs and single photon counting allows for the ultimate in accuracy and sensitivity in the acquisition of data.

Steady State measurement examples include:Steady State Fluorescence: EEMs, excitation-emission map

  • Excitation and Emission Scans
  • Synchronous Scans & Maps
  • Steady State Fluorescence Anisotropy
  • Excitation-Emission Maps (EEM)
  • Kinetic Measurements
  • Temperature Maps

Products

Applications

  • Absolute Quantum Yield Measurements
  • Steady State Singlet Oxygen Emission
  • Water Quality Assessment
  • Phosphor Powders Composition Analysis
  • Fluorescence Upconversion

Publications

AuthorYearTitleJournalAdditional detailsVolumePagesInstrumentsPublication details
Anna Gakamsky et al.2017Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular LevelScientific Reports
Volume:7
Pages:40375
Year:2017
Author:Anna Gakamsky et al.
Journal:Scientific Reports
Volume:7
Pages:40375
Year:2017
Instruments
740375Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular Level
Author:Anna Gakamsky et al.Further info
Journal:Scientific Reports
Volume:7
Pages:40375
Year:2017

Instruments

Cédric Mongin et al.2016Direct observation of triplet energy transfer from semiconductor nanocrystalsScience
Volume:351
Pages:369-372
Year:2016
Author:Cédric Mongin et al.
Journal:Science
Volume:351
Pages:369-372
Year:2016
Instruments
351369-372Direct observation of triplet energy transfer from semiconductor nanocrystals
Author:Cédric Mongin et al.Further info
Eli Zysman-Colman et al.2016Solution-Processable Silicon Phthalocyanines in Electroluminescent and Photovoltaic DevicesACS Applied Materials & Interfaces
Volume:8
Pages:9247-9253
Year:2016
Author:Eli Zysman-Colman et al.
Journal:ACS Applied Materials & Interfaces
Volume:8
Pages:9247-9253
Year:2016
Instruments
89247-9253Solution-Processable Silicon Phthalocyanines in Electroluminescent and Photovoltaic Devices
Author:Eli Zysman-Colman et al.Further info
Journal:ACS Applied Materials & Interfaces
Volume:8
Pages:9247-9253
Year:2016

Instruments

M. A. Shipman, K. J. Ramhita and B. A. Blight2016Sensing a Bacillis anthracis biomarker with well-known OLED emitter EuTta3PhenJournal of Materials Chemistry B
Volume:
Pages:
Year:2016
Author: M. A. Shipman, K. J. Ramhita and B. A. Blight
Journal:Journal of Materials Chemistry B
Volume:
Pages:
Year:2016
Instruments
Sensing a Bacillis anthracis biomarker with well-known OLED emitter EuTta3Phen
Author: M. A. Shipman, K. J. Ramhita and B. A. BlightFurther info
Journal:Journal of Materials Chemistry B
Volume:
Pages:
Year:2016

Instruments

Xixi Qin et al.2016Hybrid coordination-network-engineering for bridging cascaded channels to activate long persistent phosphorescence in the second biological windowNature Scientific Reports
Volume:6
Pages:20275
Year:2016
Author:Xixi Qin et al.
Journal:Nature Scientific Reports
Volume:6
Pages:20275
Year:2016
Instruments
620275Hybrid coordination-network-engineering for bridging cascaded channels to activate long persistent phosphorescence in the second biological window
Author:Xixi Qin et al.Further info
Journal:Nature Scientific Reports
Volume:6
Pages:20275
Year:2016

Instruments

De-Chao Yu et al.2015Multi-photon quantum cutting in Gd2O2S:Tm3+ to enhance the photo-response of solar cellsLight: Science & Applications
Volume:4
Pages:e344
Year:2015
Author:De-Chao Yu et al.
Journal:Light: Science & Applications
Volume:4
Pages:e344
Year:2015
Instruments
4e344Multi-photon quantum cutting in Gd2O2S:Tm3+ to enhance the photo-response of solar cells
Author:De-Chao Yu et al.Further info
Journal:Light: Science & Applications
Volume:4
Pages:e344
Year:2015

Instruments

Like Huanga et al.2016Efficient planar perovskite solar cells without a high temperature processed titanium dioxide electron transport layerSolar Energy Materials and Solar Cells
Volume:149
Pages:1-8
Year:2016
Author:Like Huanga et al.
Journal:Solar Energy Materials and Solar Cells
Volume:149
Pages:1-8
Year:2016
Instruments
1491-8Efficient planar perovskite solar cells without a high temperature processed titanium dioxide electron transport layer
Author:Like Huanga et al.Further info
Journal:Solar Energy Materials and Solar Cells
Volume:149
Pages:1-8
Year:2016

Instruments

Yanyan Li et al.2014A Single-Component White-Emitting CaSr2Al2O6:Ce3+, Li+, Mn2+ Phosphor via Energy TransferInorganic Chemistry
Volume:53
Pages:7668-7675
Year:2014
Author:Yanyan Li et al.
Journal:Inorganic Chemistry
Volume:53
Pages:7668-7675
Year:2014
Instruments
537668-7675A Single-Component White-Emitting CaSr2Al2O6:Ce3+, Li+, Mn2+ Phosphor via Energy Transfer
Author:Yanyan Li et al.Further info
Journal:Inorganic Chemistry
Volume:53
Pages:7668-7675
Year:2014

Instruments

Chen Liao et. al2015Bright white-light emission from Ag/SiO2/ CdS-ZnS core/ shell/shell plasmon couplersNanoscale
Volume:
Pages:
Year:2015
Author: Chen Liao et. al
Journal:Nanoscale
Volume:
Pages:
Year:2015
Instruments
Bright white-light emission from Ag/SiO2/ CdS-ZnS core/ shell/shell plasmon couplers
Author: Chen Liao et. alFurther info
Journal:Nanoscale
Volume:
Pages:
Year:2015

Instruments

Ahmad S. Sediq et al.2016No Touching! Abrasion of Adsorbed Protein Is the Root Cause of Subvisible Particle Formation During StirringJournal of Pharmaceutical Sciences
Volume:
Pages:
Year:2016
Author:Ahmad S. Sediq et al.
Journal:Journal of Pharmaceutical Sciences
Volume:
Pages:
Year:2016
Instruments
No Touching! Abrasion of Adsorbed Protein Is the Root Cause of Subvisible Particle Formation During Stirring
Author:Ahmad S. Sediq et al.Further info
Journal:Journal of Pharmaceutical Sciences
Volume:
Pages:
Year:2016

Instruments

Yuren Wang, Haiching Ma2015Protein kinase profiling assays: a technology reviewDrug Discovery Today: Technologies
Volume:18
Pages:1-8
Year:2015
Author:Yuren Wang, Haiching Ma
Journal:Drug Discovery Today: Technologies
Volume:18
Pages:1-8
Year:2015
Instruments
181-8Protein kinase profiling assays: a technology review
Author:Yuren Wang, Haiching MaFurther info
Journal:Drug Discovery Today: Technologies
Volume:18
Pages:1-8
Year:2015

Instruments

Christos Pliotas et al.2015The role of lipids in mechanosensationNature Structural & Molecular Biology
Volume:22
Pages:991-998
Year:2015
Author:Christos Pliotas et al.
Journal:Nature Structural & Molecular Biology
Volume:22
Pages:991-998
Year:2015
Instruments
22991-998The role of lipids in mechanosensation
Author:Christos Pliotas et al.Further info
Journal:Nature Structural & Molecular Biology
Volume:22
Pages:991-998
Year:2015

Instruments

Choi, M. K. et al.2015Wearable red-green-blue quantum dot light-emitting diode array using high-resolution intaglio transfer printingNature Communications
Volume:6
Pages:7149
Year:2015
Author:Choi, M. K. et al.
Journal:Nature Communications
Volume:6
Pages:7149
Year:2015
Instruments
67149Wearable red-green-blue quantum dot light-emitting diode array using high-resolution intaglio transfer printing
Author:Choi, M. K. et al.Further info
Journal:Nature Communications
Volume:6
Pages:7149
Year:2015

Instruments

Bilel Louahem M’Sabah et al.2015Amplification of light collection in solid-state dye-sensitized solar cells via the antenna effect through supramolecular assemblyPhysical Chemistry Chemical Physics
Volume:17
Pages:9910-9918
Year:2015
Author:Bilel Louahem M’Sabah et al.
Journal:Physical Chemistry Chemical Physics
Volume:17
Pages:9910-9918
Year:2015
Instruments
179910-9918Amplification of light collection in solid-state dye-sensitized solar cells via the antenna effect through supramolecular assembly
Author:Bilel Louahem M’Sabah et al.Further info
Journal:Physical Chemistry Chemical Physics
Volume:17
Pages:9910-9918
Year:2015

Instruments

Ruichan Lva et al.2015An imaging-guided platform for synergistic photodynamic/ photothermal/ chemo-therapy with pH/temperature-responsive drug releaseBiomaterials
Volume:63
Pages:115-127
Year:2015
Author:Ruichan Lva et al.
Journal:Biomaterials
Volume:63
Pages:115-127
Year:2015
Instruments
63115-127An imaging-guided platform for synergistic photodynamic/ photothermal/ chemo-therapy with pH/temperature-responsive drug release
Author:Ruichan Lva et al.Further info
Journal:Biomaterials
Volume:63
Pages:115-127
Year:2015

Instruments

Thomas J. Macdonald et al.2014Cation exchange of aqueous CuInS2 quantum dotsCrystEngComm
Volume:16
Pages:9455-9460
Year:2014
Author:Thomas J. Macdonald et al.
Journal:CrystEngComm
Volume:16
Pages:9455-9460
Year:2014
Instruments
169455-9460Cation exchange of aqueous CuInS2 quantum dots
Author:Thomas J. Macdonald et al.Further info
Journal:CrystEngComm
Volume:16
Pages:9455-9460
Year:2014

Instruments

Renato Grillo et al.2015Chitosan nanoparticles loaded the herbicide paraquat: The influence of the aquatic humic substances on the colloidal stability and toxicityJournal of Hazardous Materials
Volume:286
Pages:562-572
Year:2015
Author:Renato Grillo et al.
Journal:Journal of Hazardous Materials
Volume:286
Pages:562-572
Year:2015
Instruments
286562-572Chitosan nanoparticles loaded the herbicide paraquat: The influence of the aquatic humic substances on the colloidal stability and toxicity
Author:Renato Grillo et al.Further info
Journal:Journal of Hazardous Materials
Volume:286
Pages:562-572
Year:2015

Instruments

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