Steady State Fluorescence

Steady-State Fluorescence Spectroscopy investigates the long-term average fluorescence of a sample when irradiated with UV, Visible or near-IR Light.

Edinburgh Instruments offers a range of research-grade and analytical Steady State Spectrofluorometers. These vary in a number of ways but can be compact, benchtop or modular, and fully customisable for any type of fluorescence measurements that will meet the most demanding research requirements.

  • Absolute Quantum Yield Measurements
  • Steady State Singlet Oxygen Emission
  • Water Quality Assessment
  • Phosphor Powders Composition Analysis
  • Fluorescence Upconversion
Author Year Title Journal Vol. Pages Instrument
Anna Gakamsky et al. 2017 Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular Level Scientific Reports 7 40375
Cédric Mongin et al. 2016 Direct observation of triplet energy transfer from semiconductor nanocrystals Science 351 369-372
Eli Zysman-Colman et al. 2016 Solution-Processable Silicon Phthalocyanines in Electroluminescent and Photovoltaic Devices ACS Applied Materials & Interfaces 8 9247-9253
M. A. Shipman, K. J. Ramhita and B. A. Blight 2016 Sensing a Bacillis anthracis biomarker with well-known OLED emitter EuTta3Phen Journal of Materials Chemistry B
Xixi Qin et al. 2016 Hybrid coordination-network-engineering for bridging cascaded channels to activate long persistent phosphorescence in the second biological window Nature Scientific Reports 6 20275
De-Chao Yu et al. 2015 Multi-photon quantum cutting in Gd2O2S:Tm3+ to enhance the photo-response of solar cells Light: Science & Applications 4 e344
Like Huanga et al. 2016 Efficient planar perovskite solar cells without a high temperature processed titanium dioxide electron transport layer Solar Energy Materials and Solar Cells 149 1-8
Yanyan Li et al. 2014 A Single-Component White-Emitting CaSr2Al2O6:Ce3+, Li+, Mn2+ Phosphor via Energy Transfer Inorganic Chemistry 53 7668-7675
Chen Liao et. al 2015 Bright white-light emission from Ag/SiO2/CdS-ZnS core/shell/shell plasmon couplers Nanoscale
Ahmad S. Sediq et al. 2016 No Touching! Abrasion of Adsorbed Protein Is the Root Cause of Subvisible Particle Formation During Stirring Journal of Pharmaceutical Sciences
Yuren Wang, Haiching Ma 2015 Protein kinase profiling assays: a technology review Drug Discovery Today: Technologies 18 1-8
Christos Pliotas et al. 2015 The role of lipids in mechanosensation Nature Structural & Molecular Biology 22 991-998
Choi, M. K. et al. 2015 Wearable red-green-blue quantum dot light-emitting diode array using high-resolution intaglio transfer printing Nature Communications 6 7149
Bilel Louahem M’Sabah et al. 2015 Amplification of light collection in solid-state dye-sensitized solar cells via the antenna effect through supramolecular assembly Physical Chemistry Chemical Physics 17 9910-9918
Ruichan Lva et al. 2015 An imaging-guided platform for synergistic photodynamic/photothermal/chemo-therapy with pH/temperature-responsive drug release Biomaterials 63 115-127
Thomas J. Macdonald et al. 2014 Cation exchange of aqueous CuInS2 quantum dots CrystEngComm 16 9455-9460
Renato Grillo et al. 2015 Chitosan nanoparticles loaded the herbicide paraquat: The influence of the aquatic humic substances on the colloidal stability and toxicity Journal of Hazardous Materials 286 562-572

The fluorescence spectrum is a plot of fluorescence intensity vs. the registered wavelength (energy and frequency) at one excitation wavelength. These fluorescence intensity measurements (spectral measurements) allow the determination of the presence of fluorophores and their concentrations.

Spectral measurements are made using a technique called Single Photon Counting (SPC). All instruments manufactured by Edinburgh Instruments detect photons by using suitable Photomultiplier Tubes (PMTs) detectors. Using PMTs and single photon counting allows for the ultimate in accuracy and sensitivity in the acquisition of data.

Steady State measurement examples include:Steady State Example

  • Excitation and Emission Scans
  • Synchronous Scans & Maps
  • Steady State Fluorescence Anisotropy
  • Excitation-Emission Maps (EEM)
  • Kinetic Measurements
  • Temperature Maps