Resources Archive | Edinburgh Instruments


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Technical Note: Second Harmonic Generation Microscopy with the RMS1000 Confocal Microscope

In this technical note we discuss how the RMS1000 confocal microscope can be configured for second harmonic generation imaging.

Application Note: Multiphoton Imaging of Mouse Intestine

In this application note, an RMS1000 Confocal Microscope is used to image a tissue section of mouse intestine using two-photon excited fluorescence and second harmonic generation microscopy.

Technical Note: Two-Photon Fluorescence Microscopy with the RMS1000 Confocal Microscope

Discover how the RMS1000 can be configured for spectral and time-resolved two-photon fluorescence imaging in this technical note.

Ramacle® Software Highlight: Fast Mapping

Fast Mapping in Ramacle® enables the user to acquire Raman maps with significantly reduced acquisition times via more efficient stage movement. This Software Highlight details how it works and when the user should use it to decrease mapping times.

Application Note: Rapid Excitation Emission Matrix Analysis of Single Wall Carbon Nanotubes

Single-wall carbon nanotubes (SWCNTs) have unique electrical, thermal, mechanical, and optical properties which make them attractive for a wide variety of applications; ranging from drug delivery to battery electrodes. In this application note the FLS1000 Photoluminescence Spectrometer equipped with an InGaAs NIR camera is used to identify the chiral indexes present in a SWCNT sample using excitation emission matrix spectroscopy.

Application Note: Whisky Analysis by Raman Spectroscopy

Raman spectroscopy is an analytical technique which can be used both quantitatively and qualitatively. This application note details the quantitative use of Raman spectroscopy to determine ethanol content in samples of whisky. Qualitatively, Raman spectroscopy can also be used for whisky analysis to ensure it does not contain methanol, a toxic alcohol which can be fraudulently used in alcohol sales to boost profits.

Application Note: Molecular Beacon Probe Fluorescent Detection of DNA

Molecular beacon probes are a sequence of nucleotides (the building blocks of DNA and RNA) that can be used to fluorescently detect the presence of a specific sequence of DNA or RNA. With real-world examples as PCR quantification, in vivo RNA detection, pathogen detection and viral load quantification. The use of molecular beacons, coupled with a sensitive spectrofluorometer facilitates the measurement of extremely low concentrations of DNA or RNA. In this application note, nanomolar concentrations of cDNA were quantified using a molecular beacon while controlling the temperature of incubation and measuring the sample emission with an Edinburgh Instruments FS5 Spectrofluorometer.

Ramacle® Software Highlight: Surface Mapping

This Ramacle® Software Highlight focuses on Surface Mapping detailing why it is a necessary feature and how it works. Surface Mapping allows the user to analyse tricky, uneven sample types.

Application Note: Measuring Ethanol Content in Hand Sanitiser Using Raman Spectroscopy

Hand sanitiser needs to >60% ethanol to be effective at killing microbes on your hands. This Application Note details how Raman spectroscopy can be used to create ethanol calibration curves providing a rapid method for ensuring hand sanitisers meet the 60% requirement.

Application Note: Relative Quantum Yield of 2-Aminopyridine

Quantum yield is a fundamental photophysical parameter that describes a sample's fluorescence efficiency, and it can be measured optically via the absolute method and relative method. 2-Aminopyridine (2AMP) in sulfuric acid (H2SO4) has been previously used as a quantum yield reference standard in the UV-visible range. However, the 2AMP quantum yield values are now decades old. In this application note, we present a reinvestigation and revaluation of the quantum yield of 2AMP in 1M H2SO4, using quinine bisulphate (QBS) in 1M H2SO4 as the reference standard with an Edinburgh Instruments FS5 Spectrofluorometer.