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Time-Resolved Fluorescence

Time-Resolved Fluorescence (or Fluorescence Lifetime) Spectroscopy is an extension of Steady State Fluorescence. Fluorescence lifetimes, occurring as emissive decays from the singlet-state, can also be approximated as those decays occurring in the time region from picoseconds to nanoseconds.

When we discuss time-resolved fluorescence or fluorescence lifetimes, what we are studying is the fluorescence of a sample monitored as a function of time after excitation by a pulse of light.

  • Single and Multiple Exponential Decays
  • Time-Resolved Emission Spectroscopy (TRES)
  • Monomer-Excimer Kinetics
  • Time-Resolved Fluorescence Anisotropy
  • Solvent Relaxation Dynamics
Author Year Title Journal Vol. Pages Instrument
Ilaria Angeloni et al. 2017 Band-Edge Oscillator Strength of Colloidal CdSe/CdS Dot-in-Rods: Comparison of Absorption and Time-Resolved Fluorescence Spectroscopy Nanoscale
Anna Gakamsky et al. 2017 Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular Level Scientific Reports 7 40375
Jinhyung Park et al. 2016 Efficient eco-friendly inverted quantum dot sensitized solar cells Journal of Materials Chemistry A 4 827-837
Sagar Kesarkar et al. 2016 Near-IR Emitting Iridium(III) Complexes with Heteroaromatic β-Diketonate Ancillary Ligands for Efficient Solution-Processed OLEDs: Structure–Property Correlations Angewandte Chemie 128 2764-2768
Haiping He et al. 2016 Exciton localization in solution-processed organolead trihalide perovskites Nature Communications 7 10896
Cédric Mongin et al. 2016 Direct observation of triplet energy transfer from semiconductor nanocrystals Science 351 369-372
V. Caligiuri et al. 2016 Dielectric singularity in hyperbolic metamaterials: the inversion point of coexisting anisotropies Nature Scientific Reports 6 20002
Xixi Qin et al. 2016 Hybrid coordination-network-engineering for bridging cascaded channels to activate long persistent phosphorescence in the second biological window Nature Scientific Reports 6 20275
Yanyan Li et al. 2014 A Single-Component White-Emitting CaSr2Al2O6:Ce3+, Li+, Mn2+ Phosphor via Energy Transfer Inorganic Chemistry 53 7668-7675
Chen Liao et. al 2015 Bright white-light emission from Ag/SiO2/CdS-ZnS core/shell/shell plasmon couplers Nanoscale
Choi, M. K. et al. 2015 Wearable red-green-blue quantum dot light-emitting diode array using high-resolution intaglio transfer printing Nature Communications 6 7149
Zhang, Zuolun et al. 2015 D–π–A triarylboron compounds with tunable push–pull character achieved by modification of both the donor and acceptor moieties Chemistry - A European Journal 21 177-190
De-Chao Yu et al. 2015 Multi-photon quantum cutting in Gd2O2S:Tm3+ to enhance the photo-response of solar cells Light: Science & Applications 4 e344
Jamie C. Wang et al. 2015 Modulating Electron Transfer Dynamics at Dye–Semiconductor Interfaces via Self-Assembled Bilayers The Journal of Physical Chemistry C 119(7) 3502-3508
Catherine E. McCusker et al. 2014 Excited State Equilibrium Induced Lifetime Extension in a Dinuclear Platinum(II) Complex J. Phys. Chem. A 118 (45) 10391-10399
Raju Laishram et. al 2015 White light emitting soft materials from off-the-shelf ingredients Journal of Materials Chemistry C 23 5
Nadia Anikeeva et al. 2012 Evidence that the Density of Self Peptide-MHC Ligands Regulates T-Cell Receptor Signaling PLOS One 7 e41466

The time-resolution can be obtained in a number of ways, depending on the required sensitivity and time regions. Edinburgh Instruments employs the technique called Time-Correlated Single Photon Counting (TCSPC), for Time-Resolved Fluorescence, which is used for the acquisition of single photons and allows for time resolutions in the range of picoseconds (ps) to nanoseconds (ns).

time-domain decayThis techniques is a digital counting technique, counting photons that are time-correlated in relation to a short excitation light pulse.

In TCSPC the sample is repetitively excited using a pulsed light source with a high repetition rate. During the measurement a probability histogram builds, which relates the time between an excitation pulse (START) and the observation of the first fluorescence photon (STOP).

The fact that the time at which a fluorescence photon is incident on the detector can be defined with picosecond resolution is critical to the operation and precision of TCSPC.

To study lifetime decays slower than this (ns to seconds time range) please see Phosphorescence Lifetime.

To find out more about what TCSPC is and why we use TCSPC, please see the technical notes provided in the resources section.