Time-Resolved Fluorescence (or Fluorescence Lifetime) Spectroscopy is an extension of Steady State Fluorescence. Fluorescence lifetimes, occurring as emissive decays from the singlet-state, can also be approximated as those decays occurring in the time region from picoseconds to nanoseconds.
When we discuss time-resolved fluorescence or fluorescence lifetimes, what we are studying is the fluorescence of a sample monitored as a function of time after excitation by a pulse of light.
|Ilaria Angeloni et al.||2017||Band-Edge Oscillator Strength of Colloidal CdSe/CdS Dot-in-Rods: Comparison of Absorption and Time-Resolved Fluorescence Spectroscopy||Nanoscale||FLS920 Upgrades||Pulsed Diode Lasers – VIS/NIR|
|Anna Gakamsky et al.||2017||Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular Level||Scientific Reports||7||40375||FLS980 Spectrometer||Pulsed Diode Lasers – VIS/NIR||Pulsed LEDs – UV/VIS||Advanced FAST Software|
|Jinhyung Park et al.||2016||Efficient eco-friendly inverted quantum dot sensitized solar cells||Journal of Materials Chemistry A||4||827-837||FLS980 Spectrometer||FLS980 Upgrades|
|Sagar Kesarkar et al.||2016||Near-IR Emitting Iridium(III) Complexes with Heteroaromatic β-Diketonate Ancillary Ligands for Efficient Solution-Processed OLEDs: Structure–Property Correlations||Angewandte Chemie||128||2764-2768||FLS980 Spectrometer||Picosecond Pulsed Diode Lasers and LEDs|
|Haiping He et al.||2016||Exciton localization in solution-processed organolead trihalide perovskites||Nature Communications||7||10896||FLS920 Upgrades||Pulsed Diode Lasers – VIS/NIR|
|Cédric Mongin et al.||2016||Direct observation of triplet energy transfer from semiconductor nanocrystals||Science||351||369-372||LP920 Upgrades||FLS920 Upgrades||Mini-tau||Pulsed Diode Lasers – VIS/NIR|
|V. Caligiuri et al.||2016||Dielectric singularity in hyperbolic metamaterials: the inversion point of coexisting anisotropies||Nature Scientific Reports||6||20002||FLS920 Upgrades||Picosecond Pulsed Diode Lasers and LEDs|
|Xixi Qin et al.||2016||Hybrid coordination-network-engineering for bridging cascaded channels to activate long persistent phosphorescence in the second biological window||Nature Scientific Reports||6||20275||FLS920 Upgrades|
|Yanyan Li et al.||2014||A Single-Component White-Emitting CaSr2Al2O6:Ce3+, Li+, Mn2+ Phosphor via Energy Transfer||Inorganic Chemistry||53||7668-7675||FLS920 Upgrades||LP920 Upgrades|
|Chen Liao et. al||2015||Bright white-light emission from Ag/SiO2/CdS-ZnS core/shell/shell plasmon couplers||Nanoscale||FLS920 Upgrades|
|Choi, M. K. et al.||2015||Wearable red-green-blue quantum dot light-emitting diode array using high-resolution intaglio transfer printing||Nature Communications||6||7149||FLS980 Spectrometer||Pulsed Diode Lasers – VIS/NIR|
|Zhang, Zuolun et al.||2015||D–π–A triarylboron compounds with tunable push–pull character achieved by modification of both the donor and acceptor moieties||Chemistry - A European Journal||21||177-190||FLS920 Upgrades||Picosecond Pulsed Diode Lasers and LEDs|
|De-Chao Yu et al.||2015||Multi-photon quantum cutting in Gd2O2S:Tm3+ to enhance the photo-response of solar cells||Light: Science & Applications||4||e344||FLS920 Upgrades|
|Jamie C. Wang et al.||2015||Modulating Electron Transfer Dynamics at Dye–Semiconductor Interfaces via Self-Assembled Bilayers||The Journal of Physical Chemistry C||119(7)||3502-3508||FLS980 Spectrometer||LP920 Upgrades||Pulsed Diode Lasers – VIS/NIR|
|Catherine E. McCusker et al.||2014||Excited State Equilibrium Induced Lifetime Extension in a Dinuclear Platinum(II) Complex||J. Phys. Chem. A||118 (45)||10391-10399||Mini-tau||Pulsed Diode Lasers – VIS/NIR||LP920 Upgrades|
|Raju Laishram et. al||2015||White light emitting soft materials from off-the-shelf ingredients||Journal of Materials Chemistry C||23||5||FLS980 Spectrometer|
|Nadia Anikeeva et al.||2012||Evidence that the Density of Self Peptide-MHC Ligands Regulates T-Cell Receptor Signaling||PLOS One||7||e41466||LifeSpec II||Advanced FAST Software|
The time-resolution can be obtained in a number of ways, depending on the required sensitivity and time regions. Edinburgh Instruments employs the technique called Time-Correlated Single Photon Counting (TCSPC), for Time-Resolved Fluorescence, which is used for the acquisition of single photons and allows for time resolutions in the range of picoseconds (ps) to nanoseconds (ns).
In TCSPC the sample is repetitively excited using a pulsed light source with a high repetition rate. During the measurement a probability histogram builds, which relates the time between an excitation pulse (START) and the observation of the first fluorescence photon (STOP).
The fact that the time at which a fluorescence photon is incident on the detector can be defined with picosecond resolution is critical to the operation and precision of TCSPC.
To study lifetime decays slower than this (ns to seconds time range) please see Phosphorescence Lifetime.
To find out more about what TCSPC is and why we use TCSPC, please see the technical notes provided in the resources section.
To talk to a member of our Sales Team regarding time resolved fluorescence products, email them at firstname.lastname@example.org or use the Enquiry Form in the tab above.