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What is Fluorescence Quenching?

Fluorescence quenching is the decrease in fluorescence intensity of an emitter due to interaction with a quencher.

Constant Fraction Determination

Time-Correlated Single-Photon Counting receives non-uniform pulses from the excitation source and the sample, so how do we ensure the timing electronics precisely record arrival time? Discover how constant fraction discrimination enables accurate timing despite pulse fluctuations and system noise.

What is FLIM?

An introduction to Fluorescence Lifetime Imaging Microscopy (FLIM) and how it works.

Time Correlated Single Photon Counting - Why use TCSPC for Fluorescence Lifetime Measurements?

Time Correlated Single-Photon Counting (TCSPC) is the method of choice for fluorescence lifetime measurements. This blog post follows an introduction to Time Correlated Single Photon Counting, which answers the question; What is TCSPC?

What is Multichannel Scaling (MSC)

An introduction to Multi-Channel Scaling which is the ideal technique for identifying phosphorescent lifetimes.

Illumination Techniques in Optical Microscopy

In this Spectral School Tutorial, we introduce some of the most common optical microscopy terms and techniques, including upright and inverted microscopes, reflected and transmitted illumination modes, and brightfield and darkfield contrast mechanisms, and discuss their applicability with respect to different research areas.

What is Fluorescence Lifetime?

A first introduction to the popular method of Time-Correlated Single Photon Counting which may be used to separate multiple fluorescence lifetimes from a single sample.

Photoluminescence Spectroscopy Poster Presentation Series – TADF Emitter Characterisation

This poster presentation video illustrates how to fully characterise the photophysics of a Thermally Activated Delayed Fluorescence (TADF) material for OLED applications using an FS5 Spectrofluorometer. Spectral and time-resolved photoluminescence, quantum yield, and temperature dependence are all studied in a compact FS5 instrument.

Detectors for Raman Spectroscopy: CCDs, EMCCDs, and InGaAs Arrays

In this Spectral School tutorial, we discuss the differences between the detectors available for Raman microscopes, and how to select one based on important performance parameters such as quantum efficiency and spectral range.

Choosing Detectors for your FLS1000 Photoluminescence Spectrometer

The FLS1000 Photoluminescence Spectrometer can be equipped with up to 5 different detectors (8 if two emission monochromators are present), and there is a wide range to choose from including analogue, high-speed detectors or NIR-sensitive photomultiplier tubes (PMTs). In this blog post we help you to select the best photomultiplier detector for your application, either when you first buy an instrument or when you are considering an upgrade.