Spectral School Archives | Edinburgh Instruments
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Spectral School

What is Photoinduced Electron Transfer? 

In photoinduced electron transfer, an electron is transferred from a photoexcited donor molecule to an acceptor molecule. The process results in charge separation and the formation of a radical cation of the donor and a radical anion of the acceptor.
Spectral School

What is Dexter Energy Transfer?

Dexter energy transfer, also called electron exchange energy transfer, is a photophysical mechanism where a simultaneous exchange of electrons occurs between donor and acceptor molecules.
Spectral School

What is Förster Resonance Energy Transfer (FRET)?

Förster resonance energy transfer is a photophysical mechanism where energy is transferred from an excited molecule (the donor) to another molecule (the acceptor) via a dipole-dipole resonance interaction.
Spectral School

What is a Fluorescence Spectrometer?

A fluorescence spectrometer is used to study the fluorescence properties of a sample. It excites a sample with a specific wavelength of light and measures the intensity and spectral distribution of the resulting fluorescence emission.
Spectral School

What is a Stern-Volmer Plot?

The Stern-Volmer plot is used to study the interaction dynamics between a fluorescent emitter and a quencher.
Spectral School

What is Fluorescence Quenching?

Fluorescence quenching is the decrease in fluorescence intensity of an emitter due to interaction with a quencher.
Spectral School

Component Close-Up: Constant Fraction Discrimination

Time-Correlated Single-Photon Counting receives non-uniform pulses from the excitation source and the sample, so how do we ensure the timing electronics precisely record arrival time? Discover how constant fraction discrimination enables accurate timing despite pulse fluctuations and system noise.
Spectral School

Determining Fluorescence Lifetimes

Why and how we fit components to recorded TCSPC data? Using a variety of examples, we show how different systems need to be explored in different ways from the same TCSPC system.
Spectral School

Infrared or Raman Spectroscopy?

IR and Raman spectroscopy are two complimentary vibrational spectroscopy techniques. How to choose which one to use? Our Spectral School article explains how to tell if your samples are better suited to Raman or IR spectroscopy and why.
Spectral School

What is FLIM – Fluorescence Lifetime Imaging Microscopy?

An introduction to Fluorescence Lifetime Imaging Microscopy (FLIM) and how it works.