February 15, 2016
Reliable fluorescence standards and stable fluorescent probes for bio-analytics and chemistry are as important as sensitive indicator fluorophores that utilise the outstanding property of fluorescence of being highly sensitive to the fluorophore’s micro-environment. In this technical note we use the FS5 Spectrofluorometer to show the quenching of fluorescence with temperature. By using the extended capabilities of FS5-TCSPC we also record, for the same set of temperatures, the fluorescence lifetimes. We can show that temperature dependence of Rhodamine B is exclusively caused by dynamic quenching.