Filter results

1월 14, 2019

Proton-Coupled Electron Transfer (PCET); Spectral and Kinetic Transient Absorption Analysis of Acridine Orange with Tri-Tert-Butylphenol

Understanding fundamental photo-induced proton-coupled electron transfer (PCET) reactions of small molecules is central to research ranging from solar fuels, biological signalling, and organic electronics. By combining spectral and kinetic information of the photo-generated excited states and radical species present, researchers are able to elucidate the PCET reaction mechanisms involved in these applications. The Edinburgh Instruments LP Transient Absorption Spectrometer is the world’s only commercial system capable of recording instantaneous spectral information utilising an ICCD camera, while then easily being able to switch to measuring kinetic data on a PMT detector – all under software control. It was used to carry out the research in this application note.

4월 9, 2018

Application Note: Temperature-Dependent Quantum Yield of Chlorophyll Fluorescence in Plant Leaves

Fluorescence spectroscopy is a powerful tool to investigate the photochemistry of biological systems. In this application note the influence of temperature on the photochemistry of perennial leaves is investigated by measuring the fluorescence quantum yield over a temperature range of 77 K to 300 K using the FLS1000 equipped with the Cryosphere accessory.

FLS1000

12월 12, 2017

Application Note: Study of Fluorescence Quenching Kinetics Using Stopped-Flow

A powerful method for determining the kinetics of a reaction is by monitoring the concentration of the reactants or products over time using stopped-flow fluorescence spectroscopy. In this application note, the kinetics of NATA quenching by QBS are determined using the FLS1000 Photoluminescence Spectrometer equipped with the stopped-flow accessory.

FLS1000 FS5 LP980

6월 12, 2017

Application Note: Kinetics of Persistent Luminescence Phosphors

Persistent luminescence, commonly called afterglow, is long-lasting visible emission over several hours after ultraviolet excitation. It has many applications; ranging from glow-in-the-dark signage to in-vivo imaging for disease diagnosis and treatment. In this application note, the persistent luminescence kinetics of a doped strontium aluminate phosphor are characterised using the FLS1000 Photoluminescence spectrometer

FLS1000 FS5 LP980

2월 24, 2016

Time-Resolved Fluorescence Techniques to FRET Based Immunoassay

Förster resonance energy transfer (FRET) is a phenomenon of non-radiative transfer of the optical excitation from an excited electronic level of one molecule, called the donor, to a resonant electronic level of another molecule, called the acceptor. In this application note we show that one can increase the method sensitivity and reduce cost of the assay by applying a time-resolved fluorescence format to the FRET-based immunoassay.

454 KB

2월 24, 2016

Application of Time-Resolved Fluorescence Techniques to Fret Based Immunoassay

FLS1000 FS5 Lifespec Mini-tau

1 MB

11월 23, 2015

Lipid-Protein Interactions Revealed by Fluorescence Spectroscopy

FLS1000 FS5

11월 23, 2015

Ligand Binding Assays on the Basis of Fluorescence Anisotropy

Fluorescence anisotropy or polarisation provides a sensitive tool to measure the binding of ligands to proteins when a fluorophore is attached to the ligand. In this Application Note Polarised emission spectra were recorded in an FLS980 Fluorescence Spectrometer equipped with double excitation and emission monochromators.

FLS1000 FS5

10월 13, 2015

Lanthanide Based Up-Conversion Materials

FS5

10월 15, 2014

Red-Edge Excitation Spectroscopy of Proteins with the FS5 Spectrofluorometer

FS5