Spectral SchoolThe Stern-Volmer plot is used to study the interaction dynamics between a fluorescent emitter and a quencher.
Spectral SchoolFluorescence quenching is the decrease in fluorescence intensity of an emitter due to interaction with a quencher.
Spectral SchoolTime-Correlated Single-Photon Counting receives non-uniform pulses from the excitation source and the sample, so how do we ensure the timing electronics precisely record arrival time? Discover how constant fraction discrimination enables accurate timing despite pulse fluctuations and system noise.
Spectral SchoolWhy and how we fit components to recorded TCSPC data? Using a variety of examples, we show how different systems need to be explored in different ways from the same TCSPC system.
Spectral SchoolIR and Raman spectroscopy are two complimentary vibrational spectroscopy techniques. How to choose which one to use? Our Spectral School article explains how to tell if your samples are better suited to Raman or IR spectroscopy and why.
Spectral SchoolAn introduction to Fluorescence Lifetime Imaging Microscopy (FLIM) and how it works.
Spectral SchoolFurther exploration of Time-Correlated Single-Photon Counting, highlighting its superior characteristics over analogue techniques.
Spectral SchoolAn introduction to Multi-Channel Scaling which is the ideal technique for identifying phosphorescent lifetimes.
Spectral SchoolIn this Spectral School Tutorial, we introduce some of the most common optical microscopy terms and techniques, including upright and inverted microscopes, reflected and transmitted illumination modes, and brightfield and darkfield contrast mechanisms, and discuss their applicability with respect to different research areas.
Spectral SchoolA first introduction to the popular method of Time-Correlated Single Photon Counting which may be used to separate multiple fluorescence lifetimes from a single sample.
