UV-Vis Spectrophotometer | DS5, Dual Beam | Double Beam | UV
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DS5 Dual Beam UV-Vis Spectrophotometer

DS5 Dual Beam UV-Vis Spectrophotometer

  • Variable bandpass options at 0.5, 1.0, 1.5, 2.0 or 4.0 nm over
    the range 190 nm – 1100 nm
  • Fast scanning – up to 6,000 nm/min to aid sample analysis throughput
  • Automatic accessory recognition connected to the DS5
  • Optional Visacle® software for PC control
  • Modern touchscreen interface with intuitive menus and functions to ensure ease of use for standalone control
  • USB, SD card and internal data storage for convenient retrieval of methods and results

Product Description

DS5 Dual Beam UV-Vis Spectrophotometer

The DS5 UV-Vis Spectrophotometer is a high performance dual beam instrument that measures absorption and transmission as a function of wavelength and is suitable for many analytical applications where accuracy and precision measurements are key to your results.

Designed and manufactured in the UK, the DS5 provides a modern, user-friendly and accurate UV-Vis spectrophotometer for a wide range of sample types and measurements.

Utilising a dual lamp and Czerny-Turner configuration monochromator, the DS5 features a compact, reliable and high throughput optical system which ensures impressive spectral performance. Additional benefits include stray light, baseline flatness, wavelength and photometric accuracy and reproducibility.

Application Note – « Transmission and Absorbance Measurements of Liquid Samples »

The DS5 can be used for absorbance measurements, an example of the type of research which can be carried out can be found in our application note « Transmission and Absorbance Measurements of Liquid Samples ». You can download the full application note here.

For price details, further information or to find out how our UV-Vis spectrophotometer can assist you with your research, simply contact a member of our sales team at sales@edinst.com.

Technical Specifications

Parameter Description
Optics Czerny-Turner, Dual Beam Monochromator
Wavelength Range 190 nm – 1100 nm
Spectral Bandwidth 0.5 nm, 1 nm, 1.5 nm, 2 nm and 4 nm
Stray Light ≤0.10% T (220 nm NaI, 340 nm NaNO2)
Wavelength Accuracy ±0.1 nm
Wavelength Repeatability ±0.1 nm
Photometric Range Absorbance: -3.4 to +3.4, %T: 0 to 300, Concentration: 0,000 to 9,999
Photometric Accuracy ±0.002 Abs (0 – 0.5 Abs), ±0.004 Abs (0.5 – 1.0 Abs), ±0.008 Abs (1.0 – 2.0 Abs)
Photometric Reproducibility ±0.0003 Abs (0-0.5 Abs), ±0.0005 Abs (0.5-1 Abs), ±0.001 Abs (1.0-2 Abs)
Wavelength Scan Speed 10, 100, 200, 400, 800, 1200, 2400, 3600, 6000 nm/min
RMS Noise ≤0.00003 Abs (0 Abs, 500nm)
Baseline Stability <0.0003 Abs/hr (500 nm, 2 hour lamp warm-up period)
Baseline Flatness ±0.0009 Abs (200 nm – 950 nm)
Light Source Tungsten-Halogen and Deuterium Lamps
Light Source Switching Automatic switching selectable for 325 nm – 370 nm range
Detector Silicon Photodiode
Display 7” Touchscreen
Software Touchscreen operation, PC-controlled software or CFR compliant PC-controlled software
Dimensions 500 mm (W) × 475 mm (D) × 250 mm (H)
Net Weight 20 Kg (approx)
Power Supply 100 – 240V, 50/60 Hz, 150VA
Ambient Temperature 10°C – 35°C
Output Device USB flash drive, SD card
Interface USB PC interface


Home Screen Measurement Setup
DS5 Software Home Screen DS5 Measurement Software

UV-Vis Measurement Modes

Photometry Mode: Perform quantitative analyses in either absorbance or transmittance modes. Select from single up to 5 different individual wavelengths, nucleic acid/protein A260/A280 ratios and set up calibration curves for concentration measurements.

Time Scan: Perform kinetic measurements for time periods ranging from 10 seconds to >27 hours. Measurement intervals are factory preset and automatically selected when the scan time is set.

Wavelength scan: Perform a full spectral scan from 190 nm – 1100 nm at any of 9 incremental and preset selectable scan speeds starting from a high resolution 10 nm/min up to a maximum scan speed of 6,000 nm/min. Data is displayed as a graphical spectrum on which data analysis can be performed through the touchscreen interface with zoom, peak-valley, smoothing and other functions available through the easy to use menu interface.

  • Concentration measurement
  • Absorbance/Transmittance measurement
  • Hexavalent Chromium, Nucleic acid measurement (nucleic acid purity, nucleic acid concentration, protein concentration calculation)
  • Wavelength scan
  • Time change
  • Single and multi-wavelength photometry
Spectrum Overlay Area Calculation
Spectrum Overlay - DS% Spectrophotometer Area Calculation DS5 Spectrophotometer

Validation Functions

To ensure optimum instrument performance, self-diagnosis incorporating a number of parameters and wavelength calibration are automatically initiated upon start-up. Furthermore, the DS5 is equipped with a GLP/GMP feature for analyses requiring validation and auditing. Parameters such as wavelength accuracy, wavelength reproducibility, bandpass, baseline flatness, baseline stability and noise level can all be validated.

Data Handling

  • Scale changing, Trace, Spectrum overlay, Peak & trough detection, Smoothing, Differentiation, and Area & rate calculating
  • Internal memory, External USB, SD card
  • File output in CSV and text format

Optional Visacle® PC-controlled Software

Visacle® offers all control, validation, data analysis and reporting functionality for DS5 in one PC-operated software package. Collected data is stored in a secure database, where it can then be organised and analysed, as well as exported in .txt, .csv or PDF format.

Visacle® can be purchased as a CFR Part 11 compliant package for users requiring additional security. Features include:

  • Configurable user profiles with secure login and e-signatures
  • Audit trail logging of all actions taken
  • Permission system to restrict actions and data access to authorised users
Visacle® Project Browser Visacle® CFR User Profile Manager