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Time-Resolved Fluorescence

Time-Resolved Fluorescence (or Fluorescence Lifetime) Spectroscopy is an extension of Steady State Fluorescence. When we discuss time-resolved fluorescence or fluorescence lifetimes, what we are studying is the fluorescence of a sample monitored as a function of time after excitation by a pulse of light. Fluorescence lifetimes, occurring as emissive decays from the singlet-state, can also be approximated as those decays occurring in the time region from picoseconds to nanoseconds.

If you are conducting research on time-resolved fluorescence and would like to know how our instrumentation can help you with your research, simply contact a member of our Sales Team.

Description

The time-resolution can be obtained in a number of ways, depending on the required sensitivity and time regions. Edinburgh Instruments employs the technique called Time-Correlated Single Photon Counting (TCSPC), for Time-Resolved Fluorescence, which is used for the acquisition of single photons and allows for time resolutions in the range of picoseconds (ps) to nanoseconds (ns). time-domain decay - fluorescence lifetime diagram depicting time resolved fluorescence.

This technique is a digital counting technique, counting photons that are time-correlated in relation to a short excitation light pulse. In TCSPC the sample is repetitively excited using a pulsed light source with a high repetition rate. During the measurement a probability histogram builds, which relates the time between an excitation pulse (START) and the observation of the first fluorescence photon (STOP). The fact that the time at which a fluorescence photon is incident on the detector can be defined with picosecond resolution is critical to the operation and precision of TCSPC. To study lifetime decays slower than this (ns to seconds time range) please see Phosphorescence Lifetime.

To find out more about what TCSPC is and why we use TCSPC, please see the technical notes provided in the resources section.

 

 

Products

Applications

  • Single and Multiple Exponential Decays
  • Time-Resolved Emission Spectroscopy (TRES)
  • Monomer-Excimer Kinetics
  • Time-Resolved Fluorescence Anisotropy
  • Solvent Relaxation Dynamics

Publications

AuthorYearTitleJournalAdditional detailsVolumePagesInstrumentsPublication details
Ilaria Angeloni et al.2017Band-Edge Oscillator Strength of Colloidal CdSe/CdS Dot-in-Rods: Comparison of Absorption and Time-Resolved Fluorescence SpectroscopyNanoscale
Volume:
Pages:
Year:2017
Author:Ilaria Angeloni et al.
Journal:Nanoscale
Volume:
Pages:
Year:2017
Instruments
Band-Edge Oscillator Strength of Colloidal CdSe/CdS Dot-in-Rods: Comparison of Absorption and Time-Resolved Fluorescence Spectroscopy
Author:Ilaria Angeloni et al.Further info
Journal:Nanoscale
Volume:
Pages:
Year:2017

Instruments

Anna Gakamsky et al.2017Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular LevelScientific Reports
Volume:7
Pages:40375
Year:2017
Author:Anna Gakamsky et al.
Journal:Scientific Reports
Volume:7
Pages:40375
Year:2017
Instruments
740375Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular Level
Author:Anna Gakamsky et al.Further info
Journal:Scientific Reports
Volume:7
Pages:40375
Year:2017

Instruments

Jinhyung Park et al.2016Efficient eco-friendly inverted quantum dot sensitized solar cellsJournal of Materials Chemistry A
Volume:4
Pages:827-837
Year:2016
Author:Jinhyung Park et al.
Journal:Journal of Materials Chemistry A
Volume:4
Pages:827-837
Year:2016
Instruments
4827-837Efficient eco-friendly inverted quantum dot sensitized solar cells
Author:Jinhyung Park et al.Further info
Journal:Journal of Materials Chemistry A
Volume:4
Pages:827-837
Year:2016

Instruments

Sagar Kesarkar et al.2016Near-IR Emitting Iridium(III) Complexes with Heteroaromatic β-Diketonate Ancillary Ligands for Efficient Solution-Processed OLEDs: Structure–Property CorrelationsAngewandte Chemie
Volume:128
Pages:2764-2768
Year:2016
Author:Sagar Kesarkar et al.
Journal:Angewandte Chemie
Volume:128
Pages:2764-2768
Year:2016
Instruments
1282764-2768Near-IR Emitting Iridium(III) Complexes with Heteroaromatic β-Diketonate Ancillary Ligands for Efficient Solution-Processed OLEDs: Structure–Property Correlations
Author:Sagar Kesarkar et al.Further info
Journal:Angewandte Chemie
Volume:128
Pages:2764-2768
Year:2016

Instruments

Haiping He et al.2016Exciton localization in solution-processed organolead trihalide perovskitesNature Communications
Volume:7
Pages:10896
Year:2016
Author:Haiping He et al.
Journal:Nature Communications
Volume:7
Pages:10896
Year:2016
Instruments
710896Exciton localization in solution-processed organolead trihalide perovskites
Author:Haiping He et al.Further info
Journal:Nature Communications
Volume:7
Pages:10896
Year:2016

Instruments

Cédric Mongin et al.2016Direct observation of triplet energy transfer from semiconductor nanocrystalsScience
Volume:351
Pages:369-372
Year:2016
Author:Cédric Mongin et al.
Journal:Science
Volume:351
Pages:369-372
Year:2016
Instruments
351369-372Direct observation of triplet energy transfer from semiconductor nanocrystals
Author:Cédric Mongin et al.Further info
V. Caligiuri et al.2016Dielectric singularity in hyperbolic metamaterials: the inversion point of coexisting anisotropiesNature Scientific Reports
Volume:6
Pages:20002
Year:2016
Author:V. Caligiuri et al.
Journal:Nature Scientific Reports
Volume:6
Pages:20002
Year:2016
Instruments
620002Dielectric singularity in hyperbolic metamaterials: the inversion point of coexisting anisotropies
Author:V. Caligiuri et al.Further info
Journal:Nature Scientific Reports
Volume:6
Pages:20002
Year:2016

Instruments

Xixi Qin et al.2016Hybrid coordination-network-engineering for bridging cascaded channels to activate long persistent phosphorescence in the second biological windowNature Scientific Reports
Volume:6
Pages:20275
Year:2016
Author:Xixi Qin et al.
Journal:Nature Scientific Reports
Volume:6
Pages:20275
Year:2016
Instruments
620275Hybrid coordination-network-engineering for bridging cascaded channels to activate long persistent phosphorescence in the second biological window
Author:Xixi Qin et al.Further info
Journal:Nature Scientific Reports
Volume:6
Pages:20275
Year:2016

Instruments

Yanyan Li et al.2014A Single-Component White-Emitting CaSr2Al2O6:Ce3+, Li+, Mn2+ Phosphor via Energy TransferInorganic Chemistry
Volume:53
Pages:7668-7675
Year:2014
Author:Yanyan Li et al.
Journal:Inorganic Chemistry
Volume:53
Pages:7668-7675
Year:2014
Instruments
537668-7675A Single-Component White-Emitting CaSr2Al2O6:Ce3+, Li+, Mn2+ Phosphor via Energy Transfer
Author:Yanyan Li et al.Further info
Journal:Inorganic Chemistry
Volume:53
Pages:7668-7675
Year:2014

Instruments

Chen Liao et. al2015Bright white-light emission from Ag/SiO2/ CdS-ZnS core/ shell/shell plasmon couplersNanoscale
Volume:
Pages:
Year:2015
Author: Chen Liao et. al
Journal:Nanoscale
Volume:
Pages:
Year:2015
Instruments
Bright white-light emission from Ag/SiO2/ CdS-ZnS core/ shell/shell plasmon couplers
Author: Chen Liao et. alFurther info
Journal:Nanoscale
Volume:
Pages:
Year:2015

Instruments

Choi, M. K. et al.2015Wearable red-green-blue quantum dot light-emitting diode array using high-resolution intaglio transfer printingNature Communications
Volume:6
Pages:7149
Year:2015
Author:Choi, M. K. et al.
Journal:Nature Communications
Volume:6
Pages:7149
Year:2015
Instruments
67149Wearable red-green-blue quantum dot light-emitting diode array using high-resolution intaglio transfer printing
Author:Choi, M. K. et al.Further info
Journal:Nature Communications
Volume:6
Pages:7149
Year:2015

Instruments

Zhang, Zuolun et al.2015D–π–A triarylboron compounds with tunable push–pull character achieved by modification of both the donor and acceptor moietiesChemistry - A European Journal
Volume:21
Pages:177-190
Year:2015
Author:Zhang, Zuolun et al.
Journal:Chemistry - A European Journal
Volume:21
Pages:177-190
Year:2015
Instruments
21177-190D–π–A triarylboron compounds with tunable push–pull character achieved by modification of both the donor and acceptor moieties
Author:Zhang, Zuolun et al.Further info
Journal:Chemistry - A European Journal
Volume:21
Pages:177-190
Year:2015

Instruments

De-Chao Yu et al.2015Multi-photon quantum cutting in Gd2O2S:Tm3+ to enhance the photo-response of solar cellsLight: Science & Applications
Volume:4
Pages:e344
Year:2015
Author:De-Chao Yu et al.
Journal:Light: Science & Applications
Volume:4
Pages:e344
Year:2015
Instruments
4e344Multi-photon quantum cutting in Gd2O2S:Tm3+ to enhance the photo-response of solar cells
Author:De-Chao Yu et al.Further info
Journal:Light: Science & Applications
Volume:4
Pages:e344
Year:2015

Instruments

Jamie C. Wang et al.2015Modulating Electron Transfer Dynamics at Dye–Semiconductor Interfaces via Self-Assembled BilayersThe Journal of Physical Chemistry C
Volume:119(7)
Pages:3502-3508
Year:2015
Author:Jamie C. Wang et al.
Journal:The Journal of Physical Chemistry C
Volume:119(7)
Pages:3502-3508
Year:2015
Instruments
119(7)3502-3508Modulating Electron Transfer Dynamics at Dye–Semiconductor Interfaces via Self-Assembled Bilayers
Author:Jamie C. Wang et al.Further info
Journal:The Journal of Physical Chemistry C
Volume:119(7)
Pages:3502-3508
Year:2015

Instruments

Catherine E. McCusker et al.2014Excited State Equilibrium Induced Lifetime Extension in a Dinuclear Platinum(II) ComplexJ. Phys. Chem. A
Volume:118 (45)
Pages:10391-10399
Year:2014
Author:Catherine E. McCusker et al.
Journal:J. Phys. Chem. A
Volume:118 (45)
Pages:10391-10399
Year:2014
Instruments
118 (45)10391-10399Excited State Equilibrium Induced Lifetime Extension in a Dinuclear Platinum(II) Complex
Author:Catherine E. McCusker et al.Further info
Journal:J. Phys. Chem. A
Volume:118 (45)
Pages:10391-10399
Year:2014

Instruments

Raju Laishram et. al2015White light emitting soft materials from off-the-shelf ingredientsJournal of Materials Chemistry C
Volume:23
Pages:5
Year:2015
Author:Raju Laishram et. al
Journal:Journal of Materials Chemistry C
Volume:23
Pages:5
Year:2015
Instruments
235White light emitting soft materials from off-the-shelf ingredients
Author:Raju Laishram et. alFurther info
Journal:Journal of Materials Chemistry C
Volume:23
Pages:5
Year:2015

Instruments

Nadia Anikeeva et al.2012Evidence that the Density of Self Peptide-MHC Ligands Regulates T-Cell Receptor SignalingPLOS One
Volume:7
Pages:e41466
Year:2012
Author:Nadia Anikeeva et al.
Journal:PLOS One
Volume:7
Pages:e41466
Year:2012
Instruments
7e41466Evidence that the Density of Self Peptide-MHC Ligands Regulates T-Cell Receptor Signaling
Author:Nadia Anikeeva et al.Further info
Journal:PLOS One
Volume:7
Pages:e41466
Year:2012

Instruments

Documents

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