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RMS1000 Raman Microscope

RMS1000 Raman Microscope

  • Truly Confocal – multiple position pinhole for high spatial resolution, fluorescence and background rejection and application optimisation
  • Five-Position Grating Turrets – for unrivalled spectral resolution from <0.1 cm-1 and coverage over 5 cm-1 – 30,000 cm-1
  • Two Spectrograph Options – standard compact and long focal length spectrographs available for ultimate resolution, sensitivity, and stray light rejection
  • Four Simultaneous Detectors – up to 4 detectors can be installed, including high efficiency TE-cooled CCDs, EMCCDs, InGaAs and more
  • Photoluminescence Microscopy, Time-Resolved Measurements, Fluorescence Lifetime Imaging (FLIM) – extend the Raman capabilities to fluorescence and beyond

Product Description

The RMS1000 is an open architecture research grade confocal Raman microscope. It has been designed so it can be adapted to almost any modern, state-of-the-art Raman application.

This high-end research tool has been built with no compromises; resulting in a system that stands alone in both specification and ease of use. Applications beyond Raman such as time-resolved fluorescence microscopy and fluorescence lifetime imaging (FLIM) are all possible with the versatile RMS1000.

For our Raman microscope price details, further information or to find out how the RMS1000 Raman Microscope can assist you with your research, simply contact a member of our sales team at sales@edinst.com.

Raman Microscope Application Notes:

RMS1000 Confocal Raman Microscope

RM5 Raman Microscope

Technical Specifications

  Specifications  
LASERS Up to 5 integrated narrow-band lasers: 532 nm, 638 nm, 785 nm typically used
Additional lasers from UV to NIR are available. External lasers can be integrated
Laser selection is fully computer-controlled
Associated laser rejection filters included, fully computer-controlled
SPECTROGRAPHS Wavelength Range 200 nm – 2,200 nm
Gratings 5-position grating turrets
Slits Continuously adjustable, fully computer-controlled
SPECTRAL RESOLUTION From <0.1 cm-1 (depending on grating, laser and CCD selection)
SPATIAL RESOLUTION XY (lateral), Z (axial) 0.25 µm, <1 µm (depending on laser and microscope objective)
SPECTRAL RANGE 5 cm-1 * – 30,000 cm-1 (* with low wavenumber attachment)
CONFOCAL IMAGING Adjustable confocal pinhole, fully computer-controlled
DETECTORS CCD Detector High sensitivity ultra low noise spectroscopy CCDs
1650 x 200 pixels, TE-cooled -60°C (standard)
2000 x 256 pixels, TE-cooled -60°C (enhanced sensitivity / spectral range)
Optional Second Detector EMCCD detector, 1600 x 200 pixels, TE-cooled -100°C (fast response time)
InGaAs array, 1024 pixel, TE-cooled -90°C, up to 2,200 nm
SOFTWARE Ramacle® Comprehensive all-in-one, intuitive software package
Optional Chemometric, spectral library packages
FLUORESCENCE Spectral With low resolution grating and integrated CCD
Lifetime With picosecond pulsed lasers, TCSPC electronics, fast photon counting detectors
Spectral and lifetime fluorescence mapping is also available
LASER SAFETY Without Laser Enclosure Class 3B (depending on external laser source)
With Laser Enclosure Class 1
DIMENSIONS W x D x H From 975 mm x 610 mm x 1170 mm
Weight From 114 kg

Software

RAMACLE® SOFTWARE

Ramacle is an exceptional software package written for complete instrument control and data handling on the RMS1000 system. Ramacle controls all RMS1000 functions with a straightforward design concept. It focuses on all modern Raman spectroscopy applications, while at the same time, providing a user-friendly interface with ‘ready to publish’ outputs.

Screenshot showing example of Ramacle software.

The software provides control, visualisation, data acquisition, analysis and presentation of the RMS1000 whether it is used for generating Raman spectra or with advanced upgrades such as Raman mapping. Ramacle easily navigates the user from microscope set up to measurement set up and offers computer-controlled measurement conditions such as laser excitation, grating, pinhole size etc.


Ramacle enables sample visualisation, live signal monitoring and parameter optimisation before every measurement. The instrument status and signal are displayed and constantly updated during measurements.

Data generated by Ramacle have a proprietary file format. This contains all measurement and instrumental properties, allowing the user to retrieve important information whenever needed and ensures data traceability. Simple input and output functions provide the required compatibility with third party data analysis or presentation packages.

KnowItAllTM Raman Identification Pro spectral library is available for material identification and advanced analysis. Data acquisition methods such as single measurements, multiple and  accumulated scans, kinetic scans and generation of maps (accessory dependent) are implemented by intuitive and in user-friendly wizards.


Raman Mapping

Mapping the Raman spectra within a sample area provides previously unavailable information about the chemical and physical differences across a sample. This can confirm the identity and presence of specific components and reveals their location and distribution within the sample. The figure demonstrates an XY Raman map of a gemstone containing two constituents. The Raman map is false coloured based on Raman bands specific to each constituent.

XY Raman map of a gemstone containing two constituents

Using Ramacle the user can use our fast-mapping technique. This uses the camera and motorised stage to the fullest, greatly reducing acquisition times. The figure below shows Raman map revealing trace amounts of paracetamol (shown in red) left behind in a fingerprint on aluminium foil. Using standard mapping this map would have taken over 20 hours, using fast mapping this acquisition took under 30 minutes, without losing any spectral or spatial quality.

Raman map revealing trace amounts of paracetamol (shown in red) left behind in a fingerprint on aluminium foil.

Mapping in Ramacle also allows measurements in the Z axis, thanks to the computer-controlled pinhole. 3D mapping enables the user to gain a depth profile of their sample and is extremely useful for analysing layers samples for quality control and layer thickness conformation. Below a 3D Raman map of a transdermal patch is shown, the different layers can clearly be differentiated.

3D Raman map of a transdermal patch.

Commonly, samples are not perfectly flat, and this can cause focus issues when Raman mapping. Using the surface mapping feature in Ramacle the user can create an in-focus white light image with set Z-positions. These Z-positions will then be used for the Raman map creating a completely in focus 2D Raman map. The image below shows the difference between a sample with and without surface mapping.

The image below shows the difference between a sample with and without surface mapping.


With Ramacle fluorescence lifetime measurement at a single point of the field of view are possible. EPL/picosecond pulsed diode lasers are employed as excitation sources and the emission wavelength of detection is selected by the spectrograph. The TCSPC electronics provides temporal (lifetime) resolution with picosecond accuracy. The image shows the wide field with the selected laser spot for excitation. The insert shows the fluorescence lifetime measurement (red) together with the instrumental response function (blue) which was measured by replacing the sample with an ultra-fast decaying fluorophore (4-DASPI).

Wide field with the selected laser spot for excitation.

Fluorescence lifetime images (FLIM) can be created using the scan stage mapping facility that comes with the RMS1000. Instead of a Raman spectrum, a fluorescence lifetime measurement is obtained for every point of the image. The powerful Ramacle software will process all fluorescence decays and will produce a map (or image) of the average lifetime or other lifetime parameters. The image shows a FLIM image of a stained pine tree section, superimposed to the brightfield image.

Using these features in Ramacle the user can acquire Raman, photoluminescence, and lifetime maps using the RMS1000. The image below shows the biochemical analysis of woody plant cell walls using our multi-modal micro-spectroscopy approach.

Biochemical analysis of woody plant cell walls using our multi-modal micro-spectroscopy approach.

 

Ramacle Key Features

  • Selection of laser and scatter optical pathways
  • Selection of excitation wavelength, gratings and exposure time
  • Sample and laser focus visualisation
  • Programmed attenuator and shutter
  • Single, accumulated and kinetic spectral acquisitions (Raman and Photoluminescence)
  • Spectral correction
  • Selection and scans of internal calibration standards and automated calibration correction
  • Data operations such as arithmetic, scaling, normalisation and baseline subtraction
  • Cosmic ray removal, cropping, smoothing
  • Automated laser alignment
  • ASCII / CSV data import / export function
  • Paste options for presentations and publications

 

Features included with upgrades:

  • Mapping features – map setup, collection and data analysis
  • Fully motorised stage – XYZ control through joystick and software
  • Autofocus
  • Fluorescence lifetime measurements
  • Polariser and analyser selection and control
  • Detectors selection
  • Laser rejection filter selection
  • External camera selection and visualisation

Upgrades

Application Notes

Downloads

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