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Guide for the Measurements of Absolute Quantum Yields of Liquid Samples

Fluorescence Quantum Yields can be measured using the relative method by comparing fluorescence parameters to a published quantum yield standard, or they can be determined by the absolute method in which the number of absorbed photons of a sample and the number of consequently emitted photons are measured.This Guide is designed to help you to determine the Fluorescence Quantum Yields (QY) of liquid samples using your FLS920 spectrometer and the Integrating Sphere accessory.