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Time-Resolved Fluorescence Techniques to FRET Based Immunoassay

Förster resonance energy transfer (FRET) is a phenomenon of non-radiative transfer of the optical excitation from an excited electronic level of one molecule, called the donor, to a resonant electronic level of another molecule, called the acceptor. In this application note we show that one can increase the method sensitivity and reduce cost of the assay by applying a time-resolved fluorescence format to the FRET-based immunoassay.