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RM5

Raman Microscope

Compact and fully automated, designed for high-performance confocal Raman imaging. Experience intuitive use with advanced spectral precision.

RM5

Raman Microscope

  • Confocality: Variable slit and multiple position adjustable pinhole for higher image definition, better fluorescence rejection and application optimisation. A truly confocal Raman microscope with the most software selectable pinhole options on the market
  • Integrated Narrowband Raman Lasers: flexibility to include up to three laser wavelengths, optimise for your application needs
  • Optically effective: Free-space coupled lasers and mirror-based spectrograph to provide unrivalled Raman measurements
  • Grating Turret: Install up to five software-controlled gratings, ensuring no compromises in spectral resolution regardless of your laser choice
  • Integrated Detectors: Two detectors can be integrated to cover all wavelength and experimental needs

Live Video Demonstration

Send us your samples, and we can measure them live in an online demonstration

Contact us to arrange an online demonstration

Laser Excitation Diversity

Up to three fully software controlled internal lasers from 405 nm – 1064 nm, minimising footprint, maximising versatility

Dual Detector

Up to two detectors including CCDs, EMCCDs, InGaAs, and PMTs to cover the full wavelength range, and experimental needs

Internal Standards

Internal silicon and neon calibrations to keep your measurements accurate

Gratings

Up to five software-controlled gratings, optimised to your laser wavelengths for both spectral range, and spectral resolution

Truly Confocal

Multi-position pinhole and slit for high spatial resolution, fluorescence and background rejection, and application optimisation

Dedicated Team

Our dedicated team of applications scientists are available assist you with your samples
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PRODUCT DESCRIPTION

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The RM5 is a compact powerhouse designed for every laboratory, from industry to academia. With advanced spectral imaging capabilities, intuitive operation, and precise confocal optics, the RM5 delivers unparalleled performance across diverse applications such as materials science, pharmaceuticals, and beyond. 

Featuring a simple yet comprehensive design, the RM5 ensures ease of operation for users at any level of experience with Raman microscopy. Ramacle® software guides users through all measurement stages without limiting any Raman capabilities, resulting in the creation of high-quality, publication-ready data. 

Configurable with up to three fully software-controlled internal lasers spanning 405 nm – 1064 nm, this system maximises experimental range while maintaining a compact footprint. Laser selection is complimented by a 5 position grating turret, where gratings are selected to perfectly match chosen lasers. This design not only saves laboratory space but also enhances ease of use, eliminating the need for users to manually activate lasers or exchange gratings. 

The RM5 enhances user experience with key features: it includes an internal silicon standard for automatic wavelength calibration, ensuring precise spectral measurements without user intervention. The confocal microscope offers the most pinhole positions available, providing flexibility for optimal measurement conditions. When integrated with a motorised stage, the software generates both 2D and 3D Raman and photoluminescence maps, along with SurfMAP® for mapping uneven samples. 

This versatility allows the RM5 to seamlessly integrate into any laboratory setting, accommodating a wide range of experiments across disciplines from biologists to chemists to physicists, making Raman microscopy accessible to all. 

PRODUCT VIDEO

   

PRODUCT DETAILS

SOFTWARE

Ramacle® is an all-encompassing software package written for ease of use and complete operation of the RM5.

Ramacle Screenshot

The software prioritises automation, reducing the need for manual adjustments and allowing users to focus more on sample analysis. From initial microscope setup, where users visualise their sample in a spacious window, to configuring laser parameters, pinhole settings, and grating selection for spectral acquisition, Ramacle handles all aspects seamlessly.

On the RM5, Ramacle comes with standard data acquisition methods such as single measurements, multiple and accumulated scans, kinetic scan, as well as a comprehensive suite of mapping techniques when upgraded to a motorised stage e.g. 2D, 3D, SurfMAP, and FastMAP. Upgrades and accessories such as temperature stages and multiwell plates unlock additional software features.

MEASUREMENT EXAMPLES

Spectral Measurements
2D Mapping
3D Mapping
SurfMAP®
Spectral Measurements

Intuitive use is at the core of Ramacle’s design. We begin by focusing your sample using the microscope setup. Once you’re satisfied with the microscope image, we can proceed to the measurement setup window. The white-light image will carry over, allowing you to click on different areas of the sample for live Raman feedback using the live option. This feature helps you verify your measurement parameters and focus. You can then take single or multiple spectra from the selected sample area.

Spectral measurements in Ramacle software

Figure 1: Ramacle Software

Additionally, kinetic series can be set up simply in Ramacle, and if using a temperature stage, temperature ramps and spectral series can also be set. The RM5 can be configured to acquire photoluminescence emission spectra. Ramacle measures spectral PL responses using the CCD camera enabling single point and mapping data to be collected. Measurements are made by selecting a low groove density grating to cover the entire emission range. The software can be operated in both wavenumber and wavelength scale making working in Raman and PL effortless.

2D Mapping

2D mapping in Ramacle allows you to track the distribution of components in your sample. All the user needs to do is determine the area of interest to be investigated. Using Ramacle the area of interest to be mapped is defined, including areas larger than the field of view using stitched images. On the microscope image the map dimensions and step size are set for XY measurements, which will then move the stage to take a Raman spectrum from each point.

2D Raman Mapping in Ramacle 2D photoluminescence mapping in ramacle

Figure 1: Raman and photoluminescence imaging of MoS2 

Raman and PL imaging can be carried out on the same sample to provide complementary information, such as in 2D materials. In the example shown MoS2 is first imaged to study the Raman spectra, which indicates layer number. Then by simply changing the grating the PL emission is revealed, which can be used to measure strain across the sample.

3D Mapping

Ramacle operates 3D mapping similarly to 2D mapping, with the added ability to define the map along the Z-axis. Thanks to the truly confocal nature of the RM5, highly resolved 3D maps can be captured. This advanced capability extends Raman mapping beyond surface analysis, constructing a detailed 3D chemical image at the micron scale.

The Ramacle software can then display the result focusing on one 2D layer, on the 2D stacks collected, or as a full volumetric. Shown below are two common examples for 3D mapping; first 3 µm polystyrene beads, and on the right we a see an inclusion in pegmatite. Green is quartz, red is anatase, and blue is an unknown inclusion.

3D mapping of polystyrene beads 3D Mapping of pegmatite

Figure 1: 3D Mapping of polystyrene bead and pegmatite

SurfMAP®

The SurfMAP® feature of Ramacle enables the user to analyse difficult samples with rough and uneven surfaces. This is done by adjusting the Z-axis to ensure the laser remains in perfect focus across the sample surface. Without this feature non-flat surfaces cannot be accurately mapped due to variation in signal caused by the laser moving out of focus to the sample.

For example, when a pharmaceutical tablet has an indentation, a lab using Raman or PL imaging to inspect the tablet’s surface after manufacturing must account for the uneven surface.

Surface mapping of a pharmaceutical tablet showing each ingredient in a different colour.

Figure 1: Raman surface mapping of a pharmaceutical tablet.

The resulting map can be displayed in 3D showing the sample height variation and, in this example, reveals the distribution of paracetamol (blue), aspirin (coral), and caffeine (green) in the tablet.

Customer Testimonials

“Our RM5 sits in a multi-user facility allowing research into a range of applications such as thin films, semiconductors, and carbon materials. The ease of use makes the instrument accessible to multiple research groups and coupled with the excellent customer support, our experience with EI has been exceptional.”
RM5 Testimonial
Prof Dr Ersundu, Yildiz Technical University

Options and Upgrades

Lasers
Gratings
Detectors
Microscope
Sample Stages
Accessories and Laser Safety
Lasers

The RM5 is built with flexibility in mind. A choice of excitation lasers and associated laser rejection filters (both edge and notch) are available depending on application requirements.

Lasers

Gratings

Gratings are chosen for optimum resolution for each laser excitation, with up to a maximum of five gratings per system.

gratings

Detectors

A choice of CCD, EMCCD and InGaAs detectors are also available dependent on requirements, with a maximum of two detectors being integrated per system.

detector

Microscope

The RM5 uses one of the most modern microscopes on the market for first class Raman microscopy. You can use the microscope beyond pure Raman microscopy; the RM5 has been designed to maintain the full capability of the microscope allowing all the necessary tools to be added for exceptional visualisation and contrast of your samples.

Brightfield, darkfield, polarised light, differential interference contrast (DIC) and fluorescence are all available. Alongside a choice of high quality microscope objectives, a high performance camera can be added to the microscope to ensure pictures of your samples (and associated Raman maps) are captured with excellent quality and resolution.

Microscope

Sample Stages

Manual or motorised stages are available. The motorised stage allows automated XYZ Raman and fluorescence maps to be obtained and generated through Ramacle. Additional sample stages are compatible with the RM5, such as temperature stages, electrochemical cells, and diamond anvil cells.

Accessories and Laser Safety

Other accessories such as a polarisation kit and a Class I laser safety enclosure are also available to further expand the capabilities, flexibility and safety of your RM5 system.

RM5_Safety-Doors_

TECHNICAL SPECIFICATIONS

Laser Specification
Spectrograph
Detectors
System
Laser Specification
LASER SPECIFICATION
LasersUp to 3 narrow-band lasers including: 532 nm, 638 nm, 785 nm
Other wavelengths available on request
Laser selection is fully computer-controlled
Laser Rejection FiltersUp to 3 laser rejection filters included
Filter exchange is fully computer-controlled
Laser Attenuation4 orders of magnitude, continuous
Fully computer-controlled
Spectrograph
SPECTROGRAPH
Spectral ResolutionFrom <0.3 cm-1
Spectral Range<50 cm-1 – 15,000 cm-1*
SpectrographType
Focal Length
Gratings
Slits
Asymmetric Czerny-Turner
225 mm
5-position grating turret, fully computer-controlled
Continuously adjustable, fully computer-controlled
Confocal ImagingAdjustable confocal pinhole, fully computer-controlled
Detectors
DETECTORS
DetectorsStandard Detector
High sensitivity ultra low noise CCD
1650 x 200 pixels, TE-cooled -60°C (standard) OR
2000 x 256 pixels, TE-cooled -60°C (enhanced sensitivity and spectral range)

Optional Second Detector EMCCD detector, InGaAs and others available on request
Selection of detectors, fully computer-controlled
System
SYSTEM
Raman PolarisationOptionalPolarisation kit available, fully computer-controlled
Internal CalibrationWavelength calibration standard (Neon)
Raman shift standard (Silicon)
Sensitivity validation standard (Silicon)
Automated laser alignment
Microscope SystemFunctionality




Full upright microscope with brightfield and darkfield illuminator
Polarisation, Differential Interference Contrast (DIC) capability and fluorescence imaging
10x and 100x objective included as standard; up to 5 can be included




Optional Objective(s)Trinocular eyepiece, embedded CMOS video camera, second video camera optional
Sample ViewingXY manual stage
Sample StageXYZ motorised stage (75 mm x 50 mm XY), confocal Raman mapping
OptionalTemperature-controlled sample stages available
SoftwareRamacle®



Comprehensive all-in-one, intuitive software package




Operating SystemWindows®
FunctionalityData acquisition, spectrograph control, graphical display, data processing
OptionalChemometric, spectral library packages - KnowItAllTM
Laser SafetyWithout Laser Enclosure
With Laser Enclosure
Class 3B
Class 1
DimensionsW x D x H †
Weight †
600 mm x 800 mm x 600 mm
† 63 kg
* depending on grating, laser and CCD selection
† without laser enclosure

PRODUCT BROCHURE

APPLICATION NOTES

  • Raman Mapping of Layer Number, Strain, and Structural Defects in Graphene
  • Raman Microscopy for Pharmaceutical Analysis Application Notes January 22, 2024
  • Discrimination of Bacteria Species Using Raman Microscopy and Principal Component Analysis
All Application Notes

DOWNLOADS

  • RM5 Raman Microscope Brochure
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