RM5 Raman Microscope
- Truly Confocal – with variable slit and multiple position adjustable pinhole for higher image definition, better fluorescence rejection and application optimisation
- Integrated Narrowband Raman Lasers – up to 3 computer controlled lasers for ease of use, enhanced stability and reduced footprint
- 5-Position Grating Turret – for unrivalled spectral resolution of <0.3 cm-1 and optimisation over the full spectral range
<50 cm-1 – 15,000 cm-1 - Integrated Detectors – up to 2, including high efficiency CCD, EMCCD and InGaAs arrays for low noise, increased speed, high sensitivity and wide spectral range
Product Description
The RM5 is a compact and fully automated Raman microscope for analytical and research purposes. The truly confocal design of the RM5 is unique to the market and offers uncompromised spectral resolution, spatial resolution, and sensitivity.
Our Raman microscope builds on the expertise of robust and proven building blocks, combined with modern optical design considerations; and a focus on function, precision and speed. The result is a modern Raman microscope that stands alone in both specifications and ease of use.
- Internal Standards and Auto-Calibration – to ensure the highest quality data at all times
- 4-Position Raman Filter Turret – fully automated notch and edge filters to match the Raman range to excitation laser wavelength
- Ramacle® Software – one powerful software package for complete system control, data acquisition, analysis and ease of upgrade
- High Performance Microscope – compatible with all the latest accessories
For pricing and further information of the RM5 Raman Microscope, simply contact a member of our sales team at sales@edinst.com.
Technical Specifications
| LASER SPECIFICATION | |
|---|---|
| Lasers | Up to 3 narrow-band lasers including: 532 nm, 638 nm, 785 nm Other wavelengths available on request Laser selection is fully computer-controlled |
| Laser Rejection Filters | Up to 3 laser rejection filters included Filter exchange is fully computer-controlled |
| Laser Attenuation | 4 orders of magnitude, continuous Fully computer-controlled |
| SPECTROGRAPH | ||
|---|---|---|
| Spectral Resolution | From <0.3 cm-1 | |
| Spectral Range | <50 cm-1 – 15,000 cm-1* | |
| Spectrograph | Type Focal Length Gratings Slits |
Asymmetric Czerny-Turner 225 mm 5-position grating turret, fully computer-controlled Continuously adjustable, fully computer-controlled |
| Confocal Imaging | Adjustable confocal pinhole, fully computer-controlled |
| DETECTORS | ||
|---|---|---|
| Detectors | Standard Detector | High sensitivity ultra low noise CCD 1650 x 200 pixels, TE-cooled -60°C (standard) OR 2000 x 256 pixels, TE-cooled -60°C (enhanced sensitivity and spectral range) |
| Optional Second Detector | EMCCD detector, InGaAs and others available on request Selection of detectors, fully computer-controlled |
| SYSTEM | ||
|---|---|---|
| Raman Polarisation | Optional | Polarisation kit available, fully computer-controlled |
| Internal Calibration | Wavelength calibration standard (Neon) Raman shift standard (Silicon) Sensitivity validation standard (Silicon) Automated laser alignment |
|
| Microscope System | Functionality
|
Full upright microscope with brightfield and darkfield illuminator Polarisation, Differential Interference Contrast (DIC) capability and fluorescence imaging 10x and 100x objective included as standard; up to 5 can be included
|
| Optional Objective(s) | Trinocular eyepiece, embedded CMOS video camera, second video camera optional | |
| Sample Viewing | XY manual stage | |
| Sample Stage | XYZ motorised stage (75 mm x 50 mm XY), confocal Raman mapping | |
| Optional | Temperature-controlled sample stages available | |
| Software | Ramacle® | Comprehensive all-in-one, intuitive software package
|
| Operating System | Windows® | |
| Functionality | Data acquisition, spectrograph control, graphical display, data processing | |
| Optional | Chemometric, spectral library packages – KnowItAllTM | |
| Laser Safety | Without Laser Enclosure With Laser Enclosure |
Class 3B Class 1 |
| Dimensions | W x D x H † Weight † |
600 mm x 800 mm x 600 mm † 63 kg * depending on grating, laser and CCD selection † without laser enclosure |
Software
RAMACLE® SOFTWARE
Ramacle is an exceptional software package written for complete instrument control and data handling on the RM5 system. Ramacle controls all RM5 functions with a straightforward design concept. It focuses on all modern Raman spectroscopy applications, while at the same time, providing a user-friendly interface with ‘ready to publish’ outputs.
The software provides control, visualisation, data acquisition, analysis and presentation of the RM5 whether it is used for generating Raman spectra or with advanced upgrades such as Raman mapping. Ramacle easily navigates the user from microscope set up to measurement set up and offers computer-controlled measurement conditions such as laser excitation, grating, pinhole size etc.
Ramacle enables sample visualisation, live signal monitoring and parameter optimisation before every measurement. The instrument status and signal are displayed and constantly updated during measurements.
Data generated by Ramacle have a proprietary file format. This contains all measurement and instrumental properties, allowing the user to retrieve important information whenever needed and ensures data traceability. Simple input and output functions provide the required compatibility with third party data analysis or presentation packages.
KnowItAllTM Raman Identification Pro spectral library is available for material identification and advanced analysis. Data acquisition methods such as single measurements, multiple and accumulated scans, kinetic scans and generation of maps (accessory dependent) are implemented by intuitive and in user-friendly wizards.
Raman Mapping
Mapping the Raman spectra within a sample area provides previously unavailable information about the chemical and physical differences across a sample. This can confirm the identity and presence of specific components and reveals their location and distribution within the sample. The figure demonstrates an XY Raman map of a gemstone containing two constituents. The Raman map is false coloured based on Raman bands specific to each constituent.
Using Ramacle the user can use our fast-mapping technique. This uses the camera and motorised stage to the fullest, greatly reducing acquisition times. The figure below shows Raman map revealing trace amounts of paracetamol (shown in red) left behind in a fingerprint on aluminium foil. Using standard mapping this map would have taken over 20 hours, using fast mapping this acquisition took under 30 minutes, without losing any spectral or spatial quality.
Mapping in Ramacle also allows measurements in the Z axis, thanks to the computer-controlled pinhole. 3D mapping enables the user to gain a depth profile of their sample and is extremely useful for analysing layers samples for quality control and layer thickness conformation. Below is a 3D Raman map of a transdermal patch is shown, the different layers can clearly be differentiated.
Commonly, samples are not perfectly flat, and this can cause focus issues when Raman mapping. Using the surface mapping feature in Ramacle the user can create an in-focus white light image with set Z-positions. These Z-positions will then be used for the Raman map creating a completely in focus 2D Raman map. The image below shows the difference between a sample with and without surface mapping.
Ramacle Key Features
- Selection of laser and scatter optical pathways
- Selection of excitation wavelength, gratings and exposure time
- Sample and laser focus visualisation
- Programmed attenuator and shutter
- Single, accumulated and kinetic spectral acquisitions
- Spectral correction
- Selection and scans of internal calibration standards and automated calibration correction
- Data operations such as arithmetic, scaling, normalisation and baseline subtraction
- Cosmic ray removal, cropping, smoothing
- Automated laser alignment
- ASCII / CSV data import / export function
- Paste options for presentations and publications
Features included with upgrade:
- Mapping features – map setup, fast mapping, surface mapping, 3D mapping, collection and data analysis
- Fully motorised stage – XYZ control through joystick and software
- Polariser and analyser selection and control
- Detectors selection
- Laser rejection filter selection
- External camera selection and visualisation
- Autofocus
Upgrades
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The RM5 is built with flexibility in mind. A choice of excitation lasers and associated laser rejection filters (both edge and notch) are available depending on application requirements.
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Gratings are chosen for optimum resolution for each laser excitation, with up to a maximum of five gratings per system
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A choice of CCD, EMCCD and InGaAs detectors are also available dependent on requirements, with a maximum of two detectors being integrated per system.
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Other accessories such as a polarisation kit and a Class I laser safety enclosure are also available to further expand the capabilities, flexibility and safety of your RM5 system.
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The RM5 uses one of the most modern microscopes on the market for first class Raman microscopy. You can use the microscope beyond pure Raman microscopy; the RM5 has been designed to maintain the full capability of the microscope allowing all the necessary tools to be added for exceptional visualisation and contrast of your samples.
Brightfield, darkfield, polarised light, differential interference contrast (DIC) and fluorescence are all available. Alongside a choice of high quality microscope objectives, a high performance camera can be added to the microscope to ensure pictures of your samples (and associated Raman maps) are captured with excellent quality and resolution.
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A choice of microscope stages, including manual and an XYZ motorised stage which allows ease of navigation around your samples and stage area. Automated Raman maps can be obtained and generated through Ramacle. Heating/cooling of stages is also available.
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Application Notes
RM5 Raman Microscope
- Confocal Raman and Photoluminescence Microscopy for Forensic Investigations
- Gemstone Identification Using Raman Microscopy
- Raman Microscopy of Graphene
- Identification of Microplastics Using Raman Spectroscopy
- Optimisation of SERS for Glucose Sensing
You can find all of our Application Notes here.