Skip to content
Skip to content
  • News
  • Events
  • eBooks
  • Blog
  • Careers
  • Contact
  • News
  • Events
  • eBooks
  • Blog
  • Careers
  • Contact
KNOWLEDGEBASE
  • About Us
  • Products

    Fluorescence Spectrometers

    • FLS1000 Photoluminescence Spectrometer
    • FS5 Spectrofluorometer
    • LifeSpec II Lifetime Spectrometer
    • Mini-tau Lifetime Spectrometer

    Raman Microscopes

    • RM5 Raman Microscope
    • RMS1000 Multimodal Confocal Microscope

    Transient Absorption

    • LP980 Transient Absorption Spectrometer

    FTIR Spectrometers

    • IR5 FTIR Spectrometer

    Lasers and LEDs

    • Pulsed Lasers
    • Gas Lasers
    • Customisation Options
    View All Products
  • Techniques
  • Applications
KNOWLEDGEBASE
Edit Content
  • About Us
  • Products
  • Techniques
  • Applications
  • Knowledgebase
  • eBooks
  • News
  • Events
  • Blog
  • Careers
  • Contact Us

TECHNIQUES

FLIM Microscopy

  • Fluorescence Spectroscopy
  • Raman Microscopy
  • FLIM Microscopy
  • UV-Vis Spectroscopy
  • Transient Absorption Spectroscopy
  • FTIR Spectroscopy
  • Fluorescence Spectroscopy
  • Raman Microscopy
  • FLIM Microscopy
  • UV-Vis Spectroscopy
  • Transient Absorption Spectroscopy
  • FTIR Spectroscopy

Capturing fluorescence lifetimes and uncovering molecular insights

FLIM Microscopy – Fluorescence Lifetime Imaging Microscopy (FLIM) measures the spatial distribution of fluorescence lifetimes across a sample. Unlike traditional fluorescence microscopy, which measures the intensity of emitted light, FLIM creates image contrast using the decay time of fluorescence, providing an additional layer of information. Read about FLIM and how our MicroPL and RMS1000 FLIM capable microscopes can support your research.

DESCRIPTION

FLIM Microscopy involves exciting a sample with a pulsed laser and then measuring the time it takes for the fluorescence to decay after excitation. In materials science, FLIM can be used to measure the spatial variation in charge carrier lifetimes across semiconductor materials and investigate loss mechanisms. In biological imaging, the sample is stained with one or more fluorophores where the fluorophore lifetime is influenced by various factors, such as the local environment, quenching effects, and interactions with other molecules.

FLIM Microscopy is used for lifetimes in the picoseconds to nanoseconds time range and utilises Time-Correlated Single Photon Counting (TCSPC). Phosphorescence Lifetime Imaging (PLIM) using Multichannel Scaling Photon Counting (MCS) is a complementary technique for measuring longer microsecond and millisecond lifetime images; however, both techniques are often collectively called FLIM. The functionality of Edinburgh Instruments fluorescence spectrometers and Raman Microscopes can be extended with both FLIM and PLIM capability.

SPECTRAL SCHOOL

  • What is FLIM?
  • What is Fluorescence Lifetime?
  • Determining Fluorescence Lifetimes
  • What is TCSPC?
  • What is MCS?

DOCUMENTS

  • Download the TCC2 Lifetime Electronics Module Datasheet
  • Photoluminescence Product Overview Flyer

PRODUCTS

MicroPL

Spectromicroscopy Upgrade

VIEW

RMS1000

Multimodal Confocal Microscope

VIEW

MicroPL

Spectromicroscopy Upgrade

VIEW

RMS1000

Multimodal Confocal Microscope

VIEW

Keep up to date with the latest from Edinburgh Instruments

Join our mailing list and keep up with our latest videos, app notes and more!

LOCATION:
  • Edinburgh Instruments Ltd.
    2 Bain Square, Kirkton Campus, Livingston, EH54 7DQ.
  • sales@edinst.com
  • +44 1506 425 300
ABOUT:
  • About Us
  • Techniques
  • Applications
  • Knowledgebase
  • About Us
  • Techniques
  • Applications
  • Knowledgebase
PRODUCTS:
  • Fluorescence Spectrometers
  • Raman Microscopes
  • UV-Vis Spectrophotometers
  • Transient Absorption
  • FTIR Spectrometers
  • Lasers and LEDs
  • Customisation Options
  • Software
  • Upgrades
  • All Products
  • Fluorescence Spectrometers
  • Raman Microscopes
  • UV-Vis Spectrophotometers
  • Transient Absorption
  • FTIR Spectrometers
  • Lasers and LEDs
  • Customisation Options
  • Software
  • Upgrades
  • All Products
LEGALS:
  • News
  • Events
  • Blog
  • Careers
  • Contact Us
  • Terms and Conditions
  • Privacy Policy
  • News
  • Events
  • Blog
  • Careers
  • Contact Us
  • Terms and Conditions
  • Privacy Policy
SOCIALS:
Youtube Linkedin X-twitter Facebook
©2024 Edinburgh Instruments. Registered in England and Wales No: 962331. VAT No: GB 271 7379 37
Manage Consent
To provide the best experiences, we use technologies like cookies to store and/or access device information. Consenting to these technologies will allow us to process data such as browsing behaviour or unique IDs on this site. Not consenting or withdrawing consent, may adversely affect certain features and functions.
Functional Always active
The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
Preferences
The technical storage or access is necessary for the legitimate purpose of storing preferences that are not requested by the subscriber or user.
Statistics
The technical storage or access that is used exclusively for statistical purposes. The technical storage or access that is used exclusively for anonymous statistical purposes. Without a subpoena, voluntary compliance on the part of your Internet Service Provider, or additional records from a third party, information stored or retrieved for this purpose alone cannot usually be used to identify you.
Marketing
The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.
Manage options Manage services Manage {vendor_count} vendors Read more about these purposes
View preferences
{title} {title} {title}