KNOWLEDGEBASE
KNOWLEDGEBASE

TECHNIQUES

Time-Resolved Fluorescence

Time-Resolved Fluorescence (or Fluorescence Lifetime) Spectroscopy is an extension of Steady State Fluorescence. When we discuss time-resolved fluorescence or fluorescence lifetimes, what we are studying is the fluorescence of a sample monitored as a function of time after excitation by a pulse of light.

Fluorescence lifetimes, occurring as emissive decays from the singlet-state, can also be approximated as those decays occurring in the time region from picoseconds to nanoseconds.

If you are conducting research on time-resolved fluorescence and would like to know how our instrumentation can help you with your research, simply contact a member of our Sales Team.

DESCRIPTION

APPLICATIONS

  • Single and Multiple Exponential Decays
  • Time-Resolved Emission Spectroscopy (TRES)
  • Monomer-Excimer Kinetics
  • Time-Resolved Fluorescence Anisotropy
  • Solvent Relaxation Dynamics

DOCUMENTS

  • Download the TCC2 Lifetime Electronics Module Datasheet
  • Photoluminescence Product Overview Flyer

PRODUCTS

FLS1000

Photoluminescence Spectrometer

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FS5

Spectrofluorometer

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LifeSpec II

Lifetime Spectrometer

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Mini-tau

Lifetime Spectrometer

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PUBLICATIONS

SpecificationsSpectralPhosphorescence LifetimeFluorescence Lifetime
Mode of OperationSingle Photon CountingTime-Resolved Single Photon Counting (Multi Channel Scaling - MCS)Time-Correlated Single Photon Counting (TCSPC)
Lifetime rangeMilliseconds to hours10 ns - 50 s *5 ps - 10 µs *
Sensitivity>35,000:1 **n/an/a
Excitation Sources
Type450 W ozone-free xenon arc lampMicrosecond flashlampPicosecond pulsed diode lasers (EPLs, HPLs) and pulsed LEDs (EPLEDs)
Spectral Range230 nm- > 1000 nm200 nm - >1000 nmDiscrete wavelengths between 250 nm - 980 nm
Pulse widthn/a1 µs - 2 µsfrom 60 ps
OptionsOzone generating lamp with spectral range
200 nm - >1000 nm		Low to medium repetition rate pulsed lasers. Variable pulse width VPL and VPLEDsNanosecond flashlamp
200 nm - >400 nm
pulse width <1 ns
* source and detector dependent
** standard water Raman measurement conditions: Excitation wavelength = 350 nm, Excitation and emission bandwidths = 5 nm, step size = 1 nm, integration time = 1 s, emission wavelength = 397 nm, noise measured at 450 nm and calculation based on the SQRT method
MonochromatorSpecifications
TypeCzerny-Turner with 'plug and play' triple grating turret
Focal Length325 mm (double monochromators: 2 x 325 mm)
Stray Light Rejection1:10-5 (single), 1:10-10 (double)
GratingsMounted to triple grating turret
Accuracy+/- 0.2 nm *
Minimum Step Size0.01 nm *
OptionSpectrographs available for operations of CCDs and diode array detectors
* grating dependent
DetectorsPMT-900PMT-1010PMT- 1400/1700HS-PMTMCP-PMTHS-HPD
Spectral Range<200 nm - 870 nm<200 nm - 1010 nm500 nm - 1700 nm<230 nm - 850 nm<200 nm - 850 nm220 nm - 870 nm
Dark Count Rate<50 cps (-20 °C)<200 cps (-20 °C)<20 kcps or <200 kps<150 cps (0 °C)<10 cps (-20 °C)<200 cps (10 °C)
Reponse Width600 ps800 ps400 ps or 800 ps180 ps<25 ps20 ps
OptionsA wide variety of other photomultipliers and analogue detectors are available up to 5,500 nm

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