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FLS1000

Photoluminescence Spectrometer

Highly modular photoluminescence spectrometer designed for ultimate sensitivity and versatility in steady-state and time-resolved photoluminescence research.

FLS1000

Photoluminescence Spectrometer

  • Ultimate Sensitivity: Highest sensitivity on the market, with a signal-to-noise ratio of 35,000:1, ensures that the weakest photoluminescence signals can be detected
  • Modular Design: Customise the configuration to suit your specific research requirements; select from different light sources, detectors, and sample holders to create the optimal configuration
  • Upgradability: Upgrade your system with additional modules as new research needs arise, protecting the investment and keeping the equipment at the cutting edge of your research
  • NIR & MIR: Characterise NIR and MIR emitters with a wide range of infrared detector options with sensitivity out to 5500 nm
  • Automation: Equipped with advanced Fluoracle® software for comprehensive control of experiments, data acquisition, analysis, and time-saving automation features
  • Micro-Spectroscopy: Transform the FLS1000 into a powerful photoluminescence microscope with the MicroPL Upgrade; enabling high-resolution mapping of microscopic samples

Live Video Demonstration

Send us your samples, and we can measure them live in an online demonstration

Contact us to arrange an online demonstration

MicroPL Upgrade

For spectral and time-resolved photoluminescence microscopy

Dedicated Team

Our dedicated team of applications scientists are available assist you with your samples

Bespoke Development

Fully customisable systems for your requirements

Faster Measurements

Increased scan speeds and leading performance

Industry Leading Sensitivity

>35,000:1 Sensitivity Specification
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PRODUCT DESCRIPTION

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The FLS1000 Spectrometer redefines excellence in steady-state and time-resolved photoluminescence spectroscopy, making it the go-to choice for both advanced research and routine lab work.

This modular system delivers precise measurements across a broad spectral range—from ultraviolet to mid-infrared (up to 5,500 nm)—and captures lifetimes from picoseconds to seconds. It also offers highly accurate absolute photoluminescence quantum yield measurements, with flexible upgrade options available at purchase or added later as your research grows.

No matter your application, the FLS1000 enables you to measure luminescence spectra and kinetics with unmatched accuracy using cutting-edge sources, detectors, and acquisition techniques. Its spacious sample chamber accommodates nearly any sample accessory with ease.

With industry-leading sensitivity of over 35,000:1 for the standard water Raman (SQRT method), this instrument excels at detecting weakly fluorescent samples.

All functions are seamlessly managed by Fluoracle®, an all-in-one software package for comprehensive data acquisition and analysis.

PRODUCT VIDEO

PRODUCT DETAILS

SOFTWARE

Figure 1: Fluoracle Software interface

Fluoracle® is a comprehensive, user-friendly software package for instrument control and data analysis at the heart of all Edinburgh Instruments fluorescence spectrometers. It provides the user with complete control of the instrument as well as third-party accessories irrespective of configuration. 

Fluoracle is Windows compatible and features intuitive setup menus, live signal display, easy switching between measurement modes and user-friendly analysis wizards. This guarantees ease of use in the operation of a modular and potentially complex spectrometer. Common measurement routines can be saved as method files to allow previous experiments to be easily repeated.  

Light sources, detectors, complex sample holders (microplate reader, XY sample stages, titrator) and cooler options (thermostated sample holders and cryostats) are fully software controlled. Fluorescence Lifetime Imaging Microscopy (FLIM) acquisition and analysis are included in Fluoracle with a MicroPL upgrade. 

Fluoracle offers the FAST add-on for the advanced analysis of fluorescence and phosphorescence decay kinetics. 

 

MEASUREMENT EXAMPLES

Steady State
Time Resolved – TCSPC
Time Resolved – MCS
PLQY
Steady State
Excitation-Emission Maps (EEM)

Besides standard excitation and emission spectra, Fluoracle can perform synchronous scans, excitation-emission maps (EEMs), and kinetic scans at a single or multiple wavelengths. The example shows an EEM acquired with a camera, which provides maximum acquisition speed acquiring a full emission spectrum in a single shot.

EEM of single-wall carbon nanotubes in aqueous surfactant solution, acquired in FLS1000 with Xenon lamp excitation and InGaAs NIR camera. Identified nanotube structures shown in map.

Figure 1: EEM of single-wall carbon nanotubes in aqueous surfactant solution, acquired in FLS1000 with Xenon lamp excitation and InGaAs NIR camera. Identified nanotube structures shown in map. 

Temperature Maps

Temperature-dependent measurements and photoluminescence quantum yield are possible with suitable accessories. Cryostats, TE-cooled cuvette holders, and external cryostages for microscopes are all compatible and controlled by Fluoracle.

Temperature-dependent photoluminescence spectrum of a halide perovskite measured in an FLS1000 with a software-controlled cryostat.

Figure 2: Temperature-dependent photoluminescence spectrum of a halide perovskite measured in an FLS1000 with a software-controlled cryostat.

Batch Measurements

Using Batch measurement mode, customised series of scans can be set for a sample and measured automatically without the presence of the user. Loops and delays between scans can be programmed easily. The batch measurements (protocols) can be saved and loaded for future use. Communication with almost any external accessory is possible via a USB COM port command interface.

Other Steady State Measurement Examples

Steady state fluorescence anisotropy, singlet oxygen detection, chromaticity measurements, upconversion photoluminescence, electroluminescence, water quality assessments, absorption, and much more.

Time Resolved – TCSPC
Exponential Decay Analysis

Acquisition and analysis of time-resolved fluorescence is easy with the FLS1000. Fluoracle offers easy measurement wizards for time-correlated single photon counting (TCSPC), as well as analysis tools for exponential decay fitting.

The analysis routine provided is based on the Marquardt-Levenberg algorithm. Up to four exponential decay components can be fitted and additional fit quality parameters are available for quality assessment, such as autocorrelation functions, the Durbin-Watson parameter and standard deviations.

Results of a reconvolution fit with tests against three multi-exponential decay models. Experimental data (green), IRF (blue), and fitted curve (red). Residuals for one-exponential and two-exponential fits are shown to illustrate the improvement in fit quality.

Figure 1: Results of a reconvolution fit with tests against three multi-exponential decay models. Experimental data (green), IRF (blue), and fitted curve (red). Residuals for one-exponential and two-exponential fits are shown to illustrate the improvement in fit quality.

Time-Resolved Emission Spectroscopy (TRES)

With Time-Resolved Emission Spectra (TRES), a family of decay curves is automatically acquired as a function of wavelength. The data slicing option in Fluoracle allows to easily convert the family of decay curves into a family of time-resolved emission spectra.

Figure 2: TRES and time-resolved emission spectra from norharmane in ethanol, 0 – 200 ns, acquired with EPLED-280 excitation in FLS1000.

Other TCSPC Measurement Examples

Time-resolved anisotropy, monomer-excimer kinetics, solvent relaxation dynamics and much more.

Time Resolved – MCS
Time-Resolved Emission of Metal Halide Hybrids

The photoluminescence emission lifetime of metal halide hybrids extends over a large time range from nanoseconds to milliseconds. In this case the method of choice for time-resolved measurements is the Multichannel Scaling (MCS), which provides speed of measurement with photon-counting sensitivity.

In the example, two TRES profiles of Tris SbMnCl were measured, the first focusing on the 0-20 µs time range with a time-resolution of 50 ns (a & b); and a second over a time range of 50 ms with a lower time-resolution of 10 µs (c & d) to capture the long timescale behaviour.

TRES of Tris SbMnCl acquired over (a) a 20 µs time range and (b) a 50 ms time range. Extracted decays at λem = 650 nm are shown in panels (b) & (d).

Figure 1: TRES of Tris SbMnCl acquired over (a) a 20 µs time range and (b) a 50 ms time range. Extracted decays at λem = 650 nm are shown in panels (b) & (d).

Phosphorescence Spectra with Gated Detection

A gated PMT detector may be used in MCS mode to discriminate between fluorescence and phosphorescence. Gating the detector allows measuring weak phosphorescence without saturating it with fluorescence light.

Fluorescence and phosphorescence spectra of a thermally activated delayed fluorescence (TADF) material, CzDBA, acquired with a gated PMT and VPL-375 source in MCS mode.

Figure 2: Fluorescence and phosphorescence spectra of a thermally activated delayed fluorescence (TADF) material, CzDBA, acquired with a gated PMT and VPL-375 source in MCS mode.

Other MCS Measurement Examples

Time-resolved singlet oxygen measurements, time-resolved FRET measurements and much more.

PLQY
Absolute Quantum Yield of PbS Quantum Dots

The QYPro Integrating Sphere enables easy measurements of absolute photoluminescence quantum yield (PLQY) in the visible and NIR range. This method is more accurate than the relative QY approach, does not require a QY standard and is readily applicable to liquids, films and powders.

Absolute PLQY measurement of PbS quantum dots in tetrachloroethylene. The range integrated for the scattering peak is highlighted in teal, and the integration range for the emission peak is highlighted in blue.

Figure 1: Absolute PLQY measurement of PbS quantum dots in tetrachloroethylene. The range integrated for the scattering peak is highlighted in teal, and the integration range for the emission peak is highlighted in blue.

Reflectance and Absorbance in Integrating Sphere

Reflectance and absorbance of solids and powders are easily acquired in an FLS1000 with QYPro integrating sphere. Synchronous scans of the sample and reference are converted into reflectance of absorbance in the Fluoracle software.

Synchronous scans of YAG:Ce phosphor in BaSO4 at various concentrations, absorbance and reflectance results.

Figure 2: Synchronous scans of YAG:Ce phosphor in BaSO4 at various concentrations, absorbance and reflectance results.

Customer Testimonials

“[The FS5 and FLS1000] are very easy to use, and the software is quite user-friendly. I constantly train staff and students in these pieces of equipment, and because of the simplicity of their use, the students can work independently quite fast.”
FS5 + FLS1000 Customer, UK
FLS1000 User

Options and Upgrades

Configuration Upgrades
Excitation Sources
Detector Options
Photoluminescence Lifetime Upgrades
Sample Holder Options
Integrating Sphere
MicroPL Upgrade
X-Ray Excited Luminescence
Circularly Polarised Luminescence
Microplate Reader
Configuration Upgrades
Double Monochromators 

The FLS1000 can be equipped with double monochromators on excitation and emission arms. Double monochromators are recommended for highly scattering, low emissive samples as they improve the system’s stray light suppression and increase the signal-to-noise ratio. A double monochromator in the emission arm allows for up to three detectors mounted simultaneously with software-based selection; two detectors can be fitted after the double monochromator and one after the first of the two monochromators. 

Geometries 

If further detectors are required, the system can be configured in a T-geometry by the addition of a separate emission monochromator. This configuration can also be useful to provide a digital detection arm and an analogue detection arm. 

 

Excitation Sources
Xenon Lamps 

450 W continuous wavelength lamps for steady state measurements. The excitation range is typically 230 nm to >1700 nm. Ozone generating lamps may be used to increase the lower range to 180 nm. 

Microsecond Flash Lamp 

μF1 and μF2: 5 W or 60 W pulsed xenon microsecond flashlamps producing short microsecond pulses for phosphorescence decay measurements. The excitation range is typically 230 nm to 1000 nm. 

Picosecond Pulsed Diode Lasers and LEDs 

We manufacture a range of picosecond pulsed laser diodes (EPL Series, HPL Series) and pulsed LEDs (EPLED Series) for Time Correlated Single Photon Counting (TCSPC) measurements, as well as variable pulsed lasers and diodes (VPL Series, VPLED Series) for Multi-Channel Scaling (MCS). Diodes are available over the UV-VIS spectrum starting at 250 nm and are pre-set with a range of repetition frequencies but can also be pulsed externally. The driver electronics are built into the light sources, eliminating the need for additional driver boxes and feature true “plug-and-play” usability. 

Visit our Pulsed Lasers and LEDs page for full details.

AGILE Tunable White Light Source 

AGILE® is a supercontinuum laser providing picosecond pulses with variable kHz to MHz repetition rates. It couples to the FLS1000 for continuous wavelength tuning from <400 nm to >2000 nm. AGILE is the ideal source for TCSPC with a wide range of excitation wavelengths and is also compatible with MCS operation. 

Find out more about AGILE  supercontinuum laser.

Ultrafast Nanosecond Flashlamp

An ultrafast nanosecond flashlamp is available for time-resolved fluorescence studies in the range of 100 ps – 50 μs. The excitation range is tuneable and gas dependent (UV-Visible). 

Continuous Wave (CW) Lasers 

Various continuous wave lasers for use with the FLS series  are available. Some CW laser sources may also be pulsed by the spectrometer to allow, for example, upconversion decays with 808 nm and 980 nm excitation to be measured. 

Detector Options
Photomultiplier Tubes (PMTs) 

Single photon counting detectors comprise a single photon counting photomultiplier with an optimised dynode chain, mounted in a light-tight cooled or un-cooled housing.  

The following PMTs are available: Standard PMTs up to 1010 nm, High speed PMTs up to 850 nm, MCP-PMT up to 850 nm, NIR-PMTs up to 1700 nm and gated PMTs. 

A high-speed hybrid photodetector covering the visible range is available. 

Analogue Detectors 

Analogue detectors are used either for high light level applications with the requirement for high dynamic range, or as alternative detectors in spectral ranges where photomultipliers are unavailable. Depending on the required application, the analogue detectors come in a variety of housings with a variety of cooling options. Analogue detectors find application to extend the spectral coverage into the NIR to 5.5 µm. 

We offer detector assemblies that are supplied for steady state fluorescence, time-resolved fluorescence and/or time-resolved phosphorescence applications. 

Photoluminescence Lifetime Upgrades
TCSPC Electronics 

The TCC2 is an electronics module for Time-Correlated Single Photon Counting (TCSPC) and Multi-Channel Scaling (MCS) in the FLS1000.  

  • TSCPC time range: 2.5 ns – 50 µs full scale, MCS time range: 5 µs – 200 s full scale 
  • From 500 to 8,000 channels 
  • Time resolution from 305 fs/channel with ultra-low time jitter of 20 ps 
MCS Electronics 

The CB1 is a multichannel scaler for spectral and time-resolved photoluminescence measurements using the MCS technique in FLS1000. 

  • 100 MHz counter for spectral measurements (up to three channels) 
  • 10 ns minimum bin width in time-resolved measurements 
  • variable threshold settings 
Sample Holder Options
Liquids 

Single Cuvette Holder: Temperature adjustable by additional water/coolant circulation, fitted with integrated probe for sample temperature monitoring by spectrometer operating software.  

Magnetic Stirrer: Magnetic Stirrer to be fitted to single cuvette holder or 3-position sample turret. The stirrer comprises three stirrer bars and a stand-alone stirrer controller. 

Multi-Position Holders: options for three or four cuvettes. 

Powders, Thin-Films and Solids 

Front-Face Sample Holders: A range of single position front-face sample holders are available for powders, thin-films and solids. These include linear staged holders, rotational holders and clamps. 

Temperature Control 

Bath/Refrigerator (-25 °C to +150 °C): Closed cycle water/coolant bath to be used with water-cooled sample holders.  

EPR Dewar (77 K): Liquid nitrogen dewar (quartz) in mounting collar for FLS1000 sample chamber with light tight seal, directly compatible to the sample chamber access flange. A sample rod, containing the sample, will be immersed into the liquid nitrogen bath thus cooling the sample to 77K. 

Liquid Nitrogen Cryostat (77 K – 300 K) or Liquid Helium Cryostat (4 K – 300 K, 4 K – 500 K, 4 K – 800 K): A range of open and closed cycle (cryogen-free) cryostats are available for the FLS1000. The cryostats are controlled by the Fluoracle software and include sample holders for cuvettes and solids. Mounting mechanics for the FLS1000 sample chamber are provided. 

TE cooled sample holders (-35 °C to +105 °C, -35 °C to +150 °C, -50 °C to +150 °C) : Thermoelectrically cooled cuvette holders are offered with a selection of temperature ranges. They are directly compatible with the FLS1000 and controlled from the Fluoracle software to acquire maps of temperature-dependent photoluminescence. 

Polarisation / Anisotropy 

Software-controlled polarisers are available in the standard (220 – 900 nm) and extended (350 nm – 2300 nm) ranges. Automated spectral and time-resolved anisotropy measurements are possible in the Fluoracle software. 

Integrating Sphere

The QYPro integrating sphere is an additional accessory to the FLS1000 for the measurement of photoluminescence quantum yield (PLQY) and spectral reflectance properties. The QYPro is mounted within the FLS1000 sample chamber ensuring no loss of signal from external coupling methods. 

The large internal diameter of the sphere, coupled with our proprietary calibration procedure, ensures accurate results from the UV to the NIR. The QYPro’s powered external sample loading mechanism makes sample exchange quick and easy. It also reduces the risk of contamination, which is vital for maintaining accurate results over the lifetime of your integrating sphere. 

The QYPro is our most flexible integrating sphere to date, with interchangeable excitation ports allowing you to measure your samples with our multitude of FLS1000 system geometries and excitation sources. The standard photoluminescence sample accessory kit provides sample mounting options for solutions, thin films, powders and bulk materials. The QYPro also comes with a gas purging kit as standard allowing you to study air-sensitive samples with ease. 

Data analysis is quick and easy using the PLQY wizard of the Fluoracle® software. 

You can view all our QYPro focussed videos on our YouTube Channel: QYPro Videos.

MicroPL Upgrade

The MicroPL upgrade allows spectral and time-resolved photoluminescence measurements of samples in the microscopic scale. The FLS1000 is upgraded with a microscope so you can finely tune both the excitation light (illumination) and the detected emission, using widefield or point excitation. 

The microscope can be supplied as either an upright and inverted microscope. Imaging cameras are available spanning the spectrum from the visible to the near-infrared, up to 1700 nm. Excitation can be provided by halogen lamps (wide field excitation), picosecond pulsed diode lasers and pulsed LEDs, supercontinuum sources and Nd:YAG lasers (lasers provide point source illumination). 

Steady state emission spectra and fluorescence lifetime measurements can be obtained from specific spots on your sample, when using appropriate lasers. Lasers can achieve a spot size of ~2 μm (objective dependent). The optional FLIM add-on includes a computer-controlled XYZ stage and unlocks special features in the Fluoracle software including advanced analysis options for maps, such as multi-component decay fitting algorithms. 

Download the MicroPL datasheet for full details.

X-Ray Excited Luminescence

The XS1 X-ray Radioluminescence Chamber is an external sample chamber housing X-ray sources (pulsed and/or CW) for time-resolved and steady-state X-Ray luminescence spectroscopy. The X-ray induced luminescence is collected by a liquid light guide which delivers the light to the FLS1000 spectrometer for detection. 

Sample holders for cuvettes, slides and powders are included. The pulsed X-ray source must be optically excited with a picosecond or nanosecond laser (EPL or HPL series) to generate X-ray pulses which can be as fast as 150 ps (laser dependent), enabling the measurement of decays in the sub-nanosecond range.

Find out more details about the XS1. 

Circularly Polarised Luminescence

The Circularly Polarised Luminesence (CPL) upgrade for the FLS1000 provides CPL spectra from chiral samples which emit circularly polarised light. The upgrade comprises a photoelastic modulator acting as an oscillating quarter-wave plate which, together with an emission polariser, selectively transmits left or right circularly polarised light.

Schematic of experimental setup for CPL measurements

The results are expressed as emission circular intensity differential (ECID), i.e. the difference in intensity of the left and right circularly polarised components; and as the dissymmetry factor glum.​

CPL spectroscopy is a valuable addition to the FLS1000 spectrometer for studying lanthanoid complexes, materials for 3D displays, security applications, as well as biosensors and pharmaceutical compounds.

Microplate Reader

The PR2 microplate reader is an external module that is attached to the FLS1000 to perform spectral or time-resolved PL measurements on microplates. It supports user-defined microplate configurations up to 384 wells. The plate reader module is coupled to the FLS1000 by means of a bifurcated optical fibre. The control of the module is fully automated from the Fluoracle software. The spectral range supported depends on spectrometer and bifurcated fibre assembly configuration. 

TECHNICAL SPECIFICATIONS

Specifications
Monochromator
Detectors
Specifications
SpecificationsSpectralPhosphorescence LifetimeFluorescence Lifetime
Mode of OperationSingle Photon CountingTime-Resolved Single Photon Counting (Multi Channel Scaling - MCS)Time-Correlated Single Photon Counting (TCSPC)
Lifetime rangeMilliseconds to hours10 ns - 50 s *5 ps - 10 µs *
Sensitivity>35,000:1 **n/an/a
Excitation Sources
Type450 W ozone-free xenon arc lampMicrosecond flashlampPicosecond pulsed diode lasers (EPLs, HPLs) and pulsed LEDs (EPLEDs)
Spectral Range230 nm- > 1000 nm200 nm - >1000 nmDiscrete wavelengths between 250 nm - 980 nm
Pulse widthn/a1 µs - 2 µsfrom 60 ps
OptionsOzone generating lamp with spectral range
200 nm - >1000 nm
Low to medium repetition rate pulsed lasers. Variable pulse width VPL and VPLEDsNanosecond flashlamp
200 nm - >400 nm
pulse width <1 ns
* source and detector dependent
** standard water Raman measurement conditions: Excitation wavelength = 350 nm, Excitation and emission bandwidths = 5 nm, step size = 1 nm, integration time = 1 s, emission wavelength = 397 nm, noise measured at 450 nm and calculation based on the SQRT method
Monochromator
MonochromatorSpecifications
TypeCzerny-Turner with 'plug and play' triple grating turret
Focal Length325 mm (double monochromators: 2 x 325 mm)
Stray Light Rejection1:10-5 (single), 1:10-10 (double)
GratingsMounted to triple grating turret
Accuracy+/- 0.2 nm *
Minimum Step Size0.01 nm *
OptionSpectrographs available for operations of CCDs and diode array detectors
* grating dependent
Detectors
DetectorsPMT-900PMT-1010PMT- 1400/1700HS-PMTMCP-PMTHS-HPD
Spectral Range<200 nm - 870 nm<200 nm - 1010 nm500 nm - 1700 nm<230 nm - 850 nm<200 nm - 850 nm220 nm - 870 nm
Dark Count Rate<50 cps (-20 °C)<200 cps (-20 °C)<20 kcps or <200 kps<150 cps (0 °C)<10 cps (-20 °C)<200 cps (10 °C)
Reponse Width600 ps800 ps400 ps or 800 ps180 ps<25 ps20 ps
OptionsA wide variety of other photomultipliers and analogue detectors are available up to 5,500 nm

PRODUCT BROCHURE

APPLICATION NOTES

  • Characterisation of NIR PbS Quantum Dots Using the FLS1000 Photoluminescence Spectrometer
  • Time-Resolved Electroluminescence Spectroscopy of a White Light Emitting Diode
  • Halide Perovskite Phase Transitions Observations Using Temperature Dependent Photoluminescence Spectroscopy
All Application Notes

DOWNLOADS

  • FLS1000 Photoluminescence Spectrometer Brochure
  • FLS1000 Photoluminescence Spectrometer Datasheet
  • FLS1000 Photoluminescence Spectrometer Techniques and Configurations
  • TCC2 Lifetime Electronics Module Datasheet
  • MicroPL Datasheet
  • Plate Reader Data Sheet
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