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The Global Analysis Sub-Routine allows one to test decay models for common (linked) lifetime parameters.
The GLSA (Global Least Square Analysis) algorithm is 100% convergent with either automatic choice of initial values or manual parameter control.
FAST offers the fitting of fluorescence decays with up to four stretched exponential terms (Kohlrausch Quenching Model). Shift and background may be left as fitting parameters, or may be fixed.
Exponential series method combined with NTSVD (Non-Negative Truncated Singular Value Decomposition) technology. The algorithm does not require predefined shapes of the distributions.
A fitting routine based on GLSA for the analysis of Förster kinetics between donors and randomly (homogeneously) distributed acceptors is included.
Fluorescence kinetics of Fluorophores embedded in micelles can often be described by the Micellar Quenching model included in FAST.
Time-resolved fluorescence anisotropy models include a spherical rotor, two spherical rotors, spherical rotor with restricted rotation and ellipsoidal rotor.
| Advanced Analysis Tools of FAST |
|---|
| Lifetime distribution analysis |
| Exponential component analysis |
| Support plane analysis for the calculation of lifetime confidence intervals |
| Global exponential component analysis |
| Stretched exponential components analysis |
| Förster kinetics analysis |
| Micellar quenching kinetics analysis |
| Analysis of time resolved fluorescence anisotropy kinetics |
