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Fluoracle controls all FLS1000, FS5, and Mini-tau series of steady state and time-resolved spectrometers with a straightforward design concept: to enable all modern photoluminescence (PL) spectroscopy applications whilst providing a user-friendly interface with ‘ready to publish’ outputs.
Whether you select a spectral scanning-only version of one of our spectrometer systems, or you go for an advanced version that includes time-correlated single photon counting (TCSPC) lifetime or quantum yield measurements, the software will provide all instrument options automatically, from data acquisition to analysis and presentation. Selection of sources and detection modes is quick and easy: it only takes one second to switch between spectral and time-resolved measurement modes. Fluoracle allows you to monitor the live signal ahead of measurement, so selecting and optimising parameters is quick and straightforward.
Fluorescence Lifetime Imaging Microscopy (FLIM) acquisition and analysis are included in Fluoracle with a MicroPL upgrade.
Fluoracle offers the FAST add-on for the advanced analysis of fluorescence and phosphorescence decay kinetics.
Thanks to Fluoracle’s signal monitoring and easy switching between measurement modes, you can characterise the spectrum, lifetime, and quantum yield of a sample in just a few minutes. See Fluoracle in action in this virtual FS5 demo:
Temperature-dependent PL emission spectra from NaY0.77Yb0.20Er0.03F4. Fluoracle controls the temperature stage so that maps are automatically acquired.
Time-resolved electroluminescence decay of a white light InGaN LED acquired with TCSPC: time-resolved emission spectrum (left) and exponential fit result at 575 nm.
Multichannel scaling (MCS) decay of CzDBA and biexponential fit result. tp = prompt fluorescence lifetime, td = delayed fluorescence lifetime.
Quantum yield measurement from upconversion nanoparticles (UCNPs). The calculated upconversion quantum yield result is 0.014%.
Time-resolved fluorescence anisotropy for a dye in glycerol at different temperatures. Instrument response function plotted in grey.
Photoluminescence spectrum and corresponding CIE 1931 chromaticity plot of α-(DMEN)PbBr4 calculated using Fluoracle.
Photoluminescence intensity map of a halide perovskite surface acquired with FluoracleMap software (included with MicroPL accessory).
Software Functionality for Steady State Spectroscopy | ||
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Measurement Modes | Control Features | Data Manipulation and Display |
Signal rates | Wavelength selection (λEX & λEM) | Arithmetic ( + , - , × , / , append ) |
Excitation spectra | Grating selection | Scaling / multiplication factor |
Emission spectra | Spectral bandwidths | Normalise |
Anisotropy spectra | Integration time per data point (1 ms – 200 s) | Baseline subtraction |
Kinetic measurements | Sample selection (multi-position sample wells) | Crop range |
Synchronous spectra | Programmed excitation shutter | Smooth |
Corrected spectra | Programmed attenuator | 2D, 3D, Contour and text |
Temperature resolved spectra map | Source and detector selection | Grid ON / OFF |
Synchronous spectra map | Online spectral data correction | Differentiation / Integration |
Excitation-emission map | Post acquisition spectral correction | Peak search |
Sample temperature monitoring | Polariser selection and orientation | Correction |
Reflection measurements | X-Y Sample stage control | Anisotropy (G factor corrected) |
Absorption measurements | Sample temperature control | Logarithmic / linear scales |
Absorptance | Sample selection (multiple cuvette holders) | Comprehensive measurement and file properties for record keeping |
Reflectance | Temperature control of sample holders | Data manipulation and ASCII / CSV output |
Batch measurements | Detector gating for phosphorescence spectra | Cursor locations |
Absolute quantum yield measurements | Cryostat control | Join, split and extract frames |
Multiple sample position and well-plate measurements | Microscope stage control | Spectral correction wizard |
Service scans | Plate reader control | Absolute quantum yield wizard |
Titrator control | Chromaticity calculation and display wizard |
Software Functionality for Time-Resolved Photoluminescence Measurements | ||
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Measurement modes | Control Features | Analysis Features |
Signal rates | Wavelength selection for excitation and emission monochromators | Full data reconvolution using a non-linear least square fitting routine: |
Manual lifetime measurement | Sample selection (multi-position sample holders) | Exponential reconvolution or simple tail fit |
Multiple lifetime measurement | Detector selection (up to 3) | 1-4 independent exponential decay times, fixed or as free fit parameters |
Instrument Response Measurement | nF920 flashlamp voltage, frequency control and gas pressure monitor | Shift parameters, fixed or as a free fit parameter |
Time resolved excitation spectra | External Laser Triggering | Background fit, fixed or as a free fit parameter |
Time resolved emission spectra | Programmable iris attenuator | Chi-squared goodness-of-fit test |
Fluorescence anisotropy | Multiple sources | Weighted residuals, Durbin-Watson parameter |
Temperature controlled lifetime measurements | Polariser selection and orientation | Autocorrelation function |
Sample temperature monitoring | Cryostat control | Anisotropy calculation |
Multiple sample position and well-plate measurements | Measurement to peak counts or preset time | Time resolved spectra |
Forward or Reverse mode | ||
CFD settings on START and STOP: threshold, zero crossing and divider | ||
Channel Selection: 512 - 8192 channels (TCSPC) and 500 - 8000 channels (MCS) | ||
Time Range Selection: 2.5 ns - 50 µs (TCSPC) and 5 ms - 1000 s (MCS) |
Specifications | Spectral | Phosphorescence Lifetime | Fluorescence Lifetime |
---|---|---|---|
Mode of Operation | Single Photon Counting | Time-Resolved Single Photon Counting (Multi Channel Scaling - MCS) | Time-Correlated Single Photon Counting (TCSPC) |
Lifetime range | Milliseconds to hours | 10 ns - 50 s * | 5 ps - 10 µs * |
Sensitivity | >35,000:1 ** | n/a | n/a |
Excitation Sources | |||
Type | 450 W ozone-free xenon arc lamp | Microsecond flashlamp | Picosecond pulsed diode lasers (EPLs, HPLs) and pulsed LEDs (EPLEDs) |
Spectral Range | 230 nm- > 1000 nm | 200 nm - >1000 nm | Discrete wavelengths between 250 nm - 980 nm |
Pulse width | n/a | 1 µs - 2 µs | from 60 ps |
Options | Ozone generating lamp with spectral range 200 nm - >1000 nm | Low to medium repetition rate pulsed lasers. Variable pulse width VPL and VPLEDs | Nanosecond flashlamp 200 nm - >400 nm pulse width <1 ns |
* source and detector dependent ** standard water Raman measurement conditions: Excitation wavelength = 350 nm, Excitation and emission bandwidths = 5 nm, step size = 1 nm, integration time = 1 s, emission wavelength = 397 nm, noise measured at 450 nm and calculation based on the SQRT method |
Monochromator | Specifications |
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Type | Czerny-Turner with 'plug and play' triple grating turret |
Focal Length | 325 mm (double monochromators: 2 x 325 mm) |
Stray Light Rejection | 1:10-5 (single), 1:10-10 (double) |
Gratings | Mounted to triple grating turret |
Accuracy | +/- 0.2 nm * |
Minimum Step Size | 0.01 nm * |
Option | Spectrographs available for operations of CCDs and diode array detectors |
* grating dependent |
Detectors | PMT-900 | PMT-1010 | PMT- 1400/1700 | HS-PMT | MCP-PMT | HS-HPD |
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Spectral Range | <200 nm - 870 nm | <200 nm - 1010 nm | 500 nm - 1700 nm | <230 nm - 850 nm | <200 nm - 850 nm | 220 nm - 870 nm |
Dark Count Rate | <50 cps (-20 °C) | <200 cps (-20 °C) | <20 kcps or <200 kps | <150 cps (0 °C) | <10 cps (-20 °C) | <200 cps (10 °C) |
Reponse Width | 600 ps | 800 ps | 400 ps or 800 ps | 180 ps | <25 ps | 20 ps |
Options | A wide variety of other photomultipliers and analogue detectors are available up to 5,500 nm |