Skip to content
Skip to content
  • News
  • Events
  • eBooks
  • Blog
  • Careers
  • Contact
  • News
  • Events
  • eBooks
  • Blog
  • Careers
  • Contact
KNOWLEDGEBASE
  • About Us
  • Products

    Fluorescence Spectrometers

    • FLS1000 Photoluminescence Spectrometer
    • FS5 Spectrofluorometer
    • LifeSpec II Lifetime Spectrometer
    • Mini-tau Lifetime Spectrometer

    Raman Microscopes

    • RM5 Raman Microscope
    • RMS1000 Multimodal Confocal Microscope

    Transient Absorption

    • LP980 Transient Absorption Spectrometer

    FTIR Spectrometers

    • IR5 FTIR Spectrometer

    Lasers and LEDs

    • Pulsed Lasers
    • Gas Lasers
    • Customisation Options
    View All Products
  • Techniques
  • Applications
KNOWLEDGEBASE
Edit Content
  • About Us
  • Products
  • Techniques
  • Applications
  • Knowledgebase
  • eBooks
  • News
  • Events
  • Blog
  • Careers
  • Contact Us
  1. Home
  2. Blog Post
  3. Map of the Month – Imaging Mouse Intestine

BLOG

Map of the Month – Imaging Mouse Intestine

  • November 1, 2022
Edit Content

Welcome to Edinburgh Instruments monthly blog celebrating our work in Raman, Photoluminescence, and Fluorescence Lifetime Imaging. Every month we will highlight our pick for Map of the Month to show how our spectrometers can be used to reveal all the hidden secrets in your samples.

November

Two-photon excited fluorescence (2PEF) and second harmonic generation (SHG) are complementary multiphoton imaging techniques for studying biological samples, for example mouse intestine. Mice and humans are similar anatomically, therefore scientific models using mice are frequently applied to human research.

Multiphoton images employ femtosecond pulsed infrared excitation light to generate shorter wavelength light to image the sample. In 2PEF, two infrared photons are simultaneously absorbed by a fluorophore promoting it to an excited state which then radiatively relaxes emitting shorter wavelength fluorescence.  In contrast, SHG is not an absorption and emission process and instead the two infrared photons combine in a non-linear optical material with a particular symmetry to generate a new photon with exactly half the wavelength of the incident photons. The versatility of the RMS1000 Raman Microscope allows external lasers to be coupled to the system for multiphoton imaging.

 two photon excited fluorescence and second harmonic generation processes

Figure 1: Two-Photon Excited Fluorescence vs Second Harmonic Generation.

2PEF and SHG signal from the sample can be obtained simultaneously on the CCD camera. The resulting multiphoton image is shown in Figure 2(a). 2PEF at 630 nm from the Alexa Fluor® 568 dye is shown in teal and reveals the structure of the intestinal villi. The area shown in pink is SHG at 520 nm from fibrillar collagen near the intestinal wall. Collagen build up in intestine wall is indicative of many health problems such as Chron’s disease and colon disease. SHG only occurs from molecular structures that are non-centrosymmetric and fibrillar collagen is a common biological structure with this property, eliciting a strong SHG response.

Figure 2: (a) 2PEF and SHG image of mouse intestine section stained with Alexa Fluor® 568 and (b) extracted spectra from two points in the image.

RMS1000

To find out more about how the RMS1000 can benefit your research, contact our sales team today who will be happy to help you.

RELATED PRODUCTS

RMS1000

Multimodal Confocal Microscope

VIEW

RESOURCES

Tags:
  • Map of the Month
  • Life Science
  • RMS1000
  • Photoluminescence
Suggested Reading:

Map of the Month – Imaging Mouse Intestine

View more »

Second Harmonic Generation Microscopy with the RMS1000 Confocal Microscope

View more »

Two-Photon Fluorescence Microscopy with the RMS1000 Confocal Microscope

View more »
Share:
PrevPrevious
NextNext

Keep up to date with the latest from Edinburgh Instruments

Join our mailing list and keep up with our latest videos, app notes and more!

LOCATION:
  • Edinburgh Instruments Ltd.
    2 Bain Square, Kirkton Campus, Livingston, EH54 7DQ.
  • sales@edinst.com
  • +44 1506 425 300
ABOUT:
  • About Us
  • Techniques
  • Applications
  • Knowledgebase
  • About Us
  • Techniques
  • Applications
  • Knowledgebase
PRODUCTS:
  • Fluorescence Spectrometers
  • Raman Microscopes
  • UV-Vis Spectrophotometers
  • Transient Absorption
  • FTIR Spectrometers
  • Lasers and LEDs
  • Customisation Options
  • Software
  • Upgrades
  • All Products
  • Fluorescence Spectrometers
  • Raman Microscopes
  • UV-Vis Spectrophotometers
  • Transient Absorption
  • FTIR Spectrometers
  • Lasers and LEDs
  • Customisation Options
  • Software
  • Upgrades
  • All Products
LEGALS:
  • News
  • Events
  • Blog
  • Careers
  • Contact Us
  • Terms and Conditions
  • Privacy Policy
  • News
  • Events
  • Blog
  • Careers
  • Contact Us
  • Terms and Conditions
  • Privacy Policy
SOCIALS:
Youtube Linkedin X-twitter Facebook
©2024 Edinburgh Instruments. Registered in England and Wales No: 962331. VAT No: GB 271 7379 37
Manage Consent
To provide the best experiences, we use technologies like cookies to store and/or access device information. Consenting to these technologies will allow us to process data such as browsing behaviour or unique IDs on this site. Not consenting or withdrawing consent, may adversely affect certain features and functions.
Functional Always active
The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
Preferences
The technical storage or access is necessary for the legitimate purpose of storing preferences that are not requested by the subscriber or user.
Statistics
The technical storage or access that is used exclusively for statistical purposes. The technical storage or access that is used exclusively for anonymous statistical purposes. Without a subpoena, voluntary compliance on the part of your Internet Service Provider, or additional records from a third party, information stored or retrieved for this purpose alone cannot usually be used to identify you.
Marketing
The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.
Manage options Manage services Manage {vendor_count} vendors Read more about these purposes
View preferences
{title} {title} {title}