Fluorescence Lifetime Standards Data Table

Fluorescence Lifetime Standards Data Table

Fluorescence lifetime standards are useful for checking the calibration of fluorescence lifetime spectrometers and accounting for the possible wavelength dependent response of detectors.  An effective fluorescence lifetime standard should have a monoexponential fluorescence decay that is independent of the choice of excitation and emission wavelength.

The recommended reference for fluorescence lifetime standards is Anal. Chem. 79 2137-2149 (2007) which was a multinational collaboration involving nine research institutions. The fluorescence lifetimes of 20 fluorophore and solvent combinations were independently measured by each research institution using time and frequency domain lifetime spectrometers to give a set of 20 accurately known fluorescence lifetimes. The mean lifetimes reported by the study and the excitation and emission wavelengths used are given in the table below.

Compound [a] Solvent τ (ns) [b] λex (nm) λem (nm )
9-cyanoanthracene methanol
cyclohexane
16 ±1
12.7 ± 0.7
295-360
295-360
400-480
400-450
anthracene methanol
cyclohexane
5.1 ± 0.3
5.3 ± 0.1
295-360
295-360
375-442
375-442
coumarin 153 methanol 4.3 ± 0.2 295-442 495-550
DPA methanol
cyclohexane
8.7 ± 0.5
7.5 ± 0.4
295-360
295-360
400-475
400-475
erythrosin B water
methanol
0.089 ± 0.003
0.47 ± 0.02
488-568
488-568
550-580
550-590
NATA water 3.1 ± 0.1 295-309 330-410
N-methylcarbazole cyclohexane 14.1 ± 0.9 290-325 350-400
POPOP cyclohexane 1.12 ± 0.04 295-360 380-450
PPO methanol
cyclohexane
1.65 ± 0.05
1.36 ± 0.04
295-330
290-325
340-400
360-450
p-terphenyl methanol
cyclohexane
1.17 ± 0.08
0.98 ± 0.03
284-315
290-315
330-380
330-390
rhodamine B water
methanol
1.74 ± 0.02
2.5 ± 0.1
488-575
295,488-568
560-630
550-630
rubrene methanol 9.9 ± 0.3 300, 488, 514 550-610
SPA water 31.2 ± 0.4 300-330 466-520

Mean lifetimes of fluorescence standards and their excitation and emission ranges. Data obtained from Anal. Chem. 79 2137-2149 (2007).1 The samples were measured at 20 °C and the solutions degassed using freeze pump thaw or inert gas bubbling.

[a] Compound Names

DPA:  9,10-diphenylanthracene
NATA: N-acetyl-L-tryptophanamide
POPOP:1,4-bis(5-phenyloxazol-2-yl)benzene
PPO: 2,5-diphenyloxazole
SPA: N-(3-sulfopropyl)acridinium

[b] The quoted lifetime is the mean value across the different measuring institutions and the error is the sample standard deviation. The solutions were degassed prior to measurement and since oxygen is a fluorescence quencher measurement of non-degassed solutions will result in shorter lifetimes than reported here.

References

  1. Boens, W. Qin, N. Basaric, J. Hofkens, M. Ameloot, J. Pouget, J.-P. Lefevre, B. Valeur, E. Gratton, M. vandeVen, N. D. Silva, Jr., Y. Engelborghs, K. Willaert, A. Sillen, G. Rumbles, D. Phillips, A. J. W. G. Visser, A. van Hoek, J. R. Lakowicz, H. Malak, and I. Gryczynski, A. G. Szabo, D. T. Krajcarski, N. Tamai, & A. Miura, Fluorescence Lifetime Standards for Time and Frequency Domain Fluorescence Spectroscopy, Anal. Chem. 79, 2137-2149 (2007)