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A fluorescence microplate reader accessory can be added to an Edinburgh Instruments Photoluminescence spectrometer for automated high-throughput measurements of spectra, phosphorescence lifetimes and fluorescence lifetimes with photon-counting sensitivity.
Two options are offered: the PR2 Plate Reader accessory for the FLS1000 Photoluminescence Spectrometer and the SC-41 Plate Reader Module for the FS5 Spectrofluorometer. Both plate readers can utilise the spectrometer’s multiple sources and detectors, as well as its monochromator to fine-tune the wavelength and bandwidth.
If the instrument is configured for time-resolved photoluminescence, these measurements are automatically enabled in the plate reader. This, together with world-leading photon counting electronics, makes the PR2 and SC-41 stand out from traditional microplate readers in terms of flexibility and performance.
The plate readers are connected to the spectrometer excitation and emission light paths via fibres. Fluoracle software allows the user to choose the source/detector and the type of measurement; spectral or time-resolved.
The user can create and store custom microplate designs in the software. Up to 384 wells can be acquired in one automated measurement.
The PR2 is the fluorescence microplate reader option for the FLS1000 Photoluminescence Spectrometer. It connects to the spectrometer sample chamber via fibres.
This is the plate reader of choice for demanding applications, benefiting from the FLS1000 premium sensitivity and stray light rejection.
The SC-41 is a compact fluorescence microplate reader module for the FS5 Spectrofluorometer.
Swapping between the SC-41 and other sample modules only takes a few seconds, making it ideal for busy labs where saving time and optimising space is crucial.
Typical Performance | PR2 | SC-41 |
---|---|---|
Repeatability (96 well) | <0.5% | <1.5% |
Repeatability (384 well) | <5% | <3% |
Crosstalk (96 well) | <0.001% | <0.1% |
Crosstalk (384 well) | <0.05% | <5% |
Well-to-well repeatability | <5% | <3% |
Sensitivity | 1 pM fluorescein | 40 pM fluorescein |
Spectral range | 250 nm – 900 nm or 400 nm – 2,000 nm (fibre dependent) | Excitation 250 nm – 900 nm Emission 250 nm – 1,650 nm |
Dimensions (mm) | 401 x 320 x 135 | 320 x 245 x 265 (internal to FS5) |
Specifications | Spectral | Phosphorescence Lifetime | Fluorescence Lifetime |
---|---|---|---|
Mode of Operation | Single Photon Counting | Time-Resolved Single Photon Counting (Multi Channel Scaling - MCS) | Time-Correlated Single Photon Counting (TCSPC) |
Lifetime range | Milliseconds to hours | 10 ns - 50 s * | 5 ps - 10 µs * |
Sensitivity | >35,000:1 ** | n/a | n/a |
Excitation Sources | |||
Type | 450 W ozone-free xenon arc lamp | Microsecond flashlamp | Picosecond pulsed diode lasers (EPLs, HPLs) and pulsed LEDs (EPLEDs) |
Spectral Range | 230 nm- > 1000 nm | 200 nm - >1000 nm | Discrete wavelengths between 250 nm - 980 nm |
Pulse width | n/a | 1 µs - 2 µs | from 60 ps |
Options | Ozone generating lamp with spectral range 200 nm - >1000 nm | Low to medium repetition rate pulsed lasers. Variable pulse width VPL and VPLEDs | Nanosecond flashlamp 200 nm - >400 nm pulse width <1 ns |
* source and detector dependent ** standard water Raman measurement conditions: Excitation wavelength = 350 nm, Excitation and emission bandwidths = 5 nm, step size = 1 nm, integration time = 1 s, emission wavelength = 397 nm, noise measured at 450 nm and calculation based on the SQRT method |
Monochromator | Specifications |
---|---|
Type | Czerny-Turner with 'plug and play' triple grating turret |
Focal Length | 325 mm (double monochromators: 2 x 325 mm) |
Stray Light Rejection | 1:10-5 (single), 1:10-10 (double) |
Gratings | Mounted to triple grating turret |
Accuracy | +/- 0.2 nm * |
Minimum Step Size | 0.01 nm * |
Option | Spectrographs available for operations of CCDs and diode array detectors |
* grating dependent |
Detectors | PMT-900 | PMT-1010 | PMT- 1400/1700 | HS-PMT | MCP-PMT | HS-HPD |
---|---|---|---|---|---|---|
Spectral Range | <200 nm - 870 nm | <200 nm - 1010 nm | 500 nm - 1700 nm | <230 nm - 850 nm | <200 nm - 850 nm | 220 nm - 870 nm |
Dark Count Rate | <50 cps (-20 °C) | <200 cps (-20 °C) | <20 kcps or <200 kps | <150 cps (0 °C) | <10 cps (-20 °C) | <200 cps (10 °C) |
Reponse Width | 600 ps | 800 ps | 400 ps or 800 ps | 180 ps | <25 ps | 20 ps |
Options | A wide variety of other photomultipliers and analogue detectors are available up to 5,500 nm |
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