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Ramacle®

Software for the RM5 and RMS1000

Effortless operation for all your Raman microscopy needs, with seamless automation and advanced mapping techniques.

Ramacle®

Software for the RM5 and RMS1000

  • All-Encompassing Software: Enables complete instrument control and data handling for all techniques on the RM5 and the RMS1000, adapting to diverse research needs.
  • Automation Focus: Prioritises automation, reducing the need for manual adjustments and allowing users to concentrate on sample analysis.
  • Intuitive Interface: Ramacle ensures seamless navigation from setup to advanced measurements, optimising ease of use and efficiency for all users.
  • Standard capabilities: spectral measurements, cosmic ray wizard, data processing, 2D and 3D mapping, SurfMAP, FastMAP
  • Enhanced capabilities: accessory integration e.g., temperature measurements, RMS1000 multimodal techniques such as RaFLIM

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PRODUCT DESCRIPTION

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Ramacle is an exceptional software package written for complete instrument control and data handling on Raman systems. Ramacle controls all Raman microscope functions with a straightforward design concept. It focuses on all modern Raman spectroscopy applications, while at the same time, providing a user-friendly interface with ‘ready to publish’ outputs.

PRODUCT VIDEO

Software Features

Spectral Measurements
2D Mapping
3D Mapping
FastMAP
SurfMAP
RaFLIM (RMS1000 only)
Spectral Measurements

Ramacle simplifies the user journey from initially bringing their sample into focus using the microscope setup to performing spectral measurements. In the microscope setup phase, users can effortlessly adjust parameters to achieve optimal focus. Once the sample is in focus, the spectral measurement window allows comprehensive control over the laser path, including laser selection, laser power adjustment, and polarization settings.

Moving to the scatter path, Ramacle facilitates control over crucial components such as gratings, analysers, pinhole size, slit width, and detector settings. Each of these elements can be fine-tuned to tailor the measurement setup to the specific requirements of the experiment.

When ready to proceed with the measurement, users can finalise parameters such as exposure time, the number of acquisitions, and configure kinetic scans directly within the software interface. This streamlined process ensures that users can efficiently set up and execute measurements with precision and ease, enhancing the overall user experience with our instruments.

2D Mapping

2D Raman mapping in Ramacle allows users to visualise the distribution of components across a sample. By defining the area of interest and setting the map dimensions and step size, the software moves the stage to capture Raman spectrum at each point. This technique provides detailed spatial information about the sample’s chemical composition.

3D Mapping

3D Raman mapping in Ramacle extends the capabilities of 2D mapping by including the Z-axis, enabling users to probe into their samples such as  layered plastics or inclusions. By defining the volume of interest, the software captures Raman spectrum at multiple depths, constructing a detailed 3D chemical image.

Examples of Ramacle 3D Map feature

The result can focus in 2D on one particular layer, in 3D stacks, or as a complete volumetric representation to visualise distribution. Shown above a transdermal patch with several polymer layers surrounding the active ingredient.

FastMAP

In Raman mapping, the sample is placed on a motorised stage, and the user defines the mapping area and resolution. Standard mapping involves stopping the stage at each point to acquire the Raman spectrum, which is necessary for samples needing longer exposure times (>0.5 seconds). However, for samples with strong Raman responses using shorter exposure times (<0.5 seconds), this method can be inefficient due to the time spent on stage movement.

Ramacle’s FastMAP feature addresses this by continuously moving the stage during mapping, significantly reducing total acquisition time without compromising map quality. This is ideal for samples with strong Raman or photoluminescence responses, where shorter exposure times are sufficient. Fast Mapping utilizes a fast communication protocol between the stage and the CCD camera, enabling even shorter exposure times and further enhancing efficiency. FastMAP can be used in 2D and 3D spectral mapping.

SurfMAP

In standard Raman mapping, the laser is focused on the sample surface and rastered across it to collect Raman scatter from each X-Y point. This process works best with perfectly flat samples, but many samples have uneven surfaces, causing the laser to fall out of focus and reducing Raman intensity and spatial resolution. For instance, a pharmaceutical tablet with a dip in the center would show significant intensity differences due to the laser being out of focus, not sample variation.

Ramacle’s Surface Mapping feature addresses this issue by ensuring the laser remains in focus across uneven surfaces. First, a series of white light images at different Z-points are taken to cover the sample’s height variation, creating an optimally focused image and a 3D topography view. Ramacle then uses these focused Z-coordinates to adjust the stage’s Z-position at each X-Y point, maintaining focus throughout the acquisition. The resulting map can be displayed in 3D, showing sample height variation and revealing the distribution of components like paracetamol, aspirin, and caffeine in a pharmaceutical tablet.

RaFLIM (RMS1000 only)

Ramacle integrates Raman and FLIM (Fluorescence Lifetime Imaging Microscopy) measurements, providing comprehensive imaging from a single platform. FLIM measurements complement Raman data by providing spatial information about the fluorescence decay times within the sample. By combining Raman and FLIM, Ramacle offers a powerful tool for multi-dimensional analysis, enabling researchers to correlate chemical composition with fluorescence dynamics, leading to a deeper understanding of complex samples. For example below on a sample of woody plant cell walls.

TECHNICAL SPECIFICATIONS

Ramacle Key Features
Ramacle Key Features
Software Feature Description
Microscope setupUser control over camera settings microscopy viewing
Motorised stage user control over stage position
Objectives selectable by user to provide accurate scale bar
Laser viewable for alignment and focus purposes
Measurement setupUser control over all parameters to take a measurement: laser, laser power, grating, centre wavelength, pinhole, slit, exposure time, accumulation
Live, single, and Kinect scans all managed in measurement set up
Data processingSeveral data tools such as cropping, smoothing, background removal, manual baseline subtraction, normalisation
Cosmic Ray Wizard Intuitive wizard for auto-removal of cosmic rays on single spectra and maps
2D MapStandard feature with motorised stage
User can set up 2D maps in one field of view or stitch several together for larger area mapping defining measurement area and parameters. User is guided from image set up through to spectral acquisition for ease of use
3D MapStandard feature with motorised stage
User can set up 3D maps in one field of view or stitch several together for larger area mapping defining measurement area and parameters. User is guided from image set up through to spectral acquisition for ease of use
FastMAPStandard feature with motorised stage
2D and 3D maps can be operated in FastMAP mode if exposure times are <0.5 seconds, option displayed on 2D and 3D mapping set up windows
SurfMAPStandard feature with motorised stage
Using white light focusing sample remains in focus throughout measurement.
Map analysis Numerous analysis tools for false colouring of map date, including peak position, intensity, ratio, match data
FLIM (RMS1000 only)With FLIM upgrade user has a simple interface to set up fluorescence life time measurements, both single point and mapping are available. 2D, 3D, and SurfMAP are available with the upgrade.
Data analysis of lifetimes is also accessible in Ramacle.
Internal auto-calibration reference Si autocalibration feature for all lasers and gratings
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