Plate Readers

Microplate reader accessories for Edinburgh Instruments spectrometers

High throughput – fast, automated measurements of microplates with photon-counting sensitivity
Plug & Play – easily exchanged with other sample holders in the FLS1000 Photoluminescence Spectrometer or FS5 Spectrofluorometer
Fluorescence spectra – acquire spectra with high resolution enabled by the FLS1000 or FS5 monochromators
Time-resolved photoluminescence – photon-counting fluorescence (sub-ns) and phosphorescence lifetimes
Up to 384 wells – Users can customise plate layouts with up to 384 wells
Software control – Controlled from the Fluoracle^® instrument software, no additional packages needed

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Product Description

A fluorescence microplate reader accessory can be added to an Edinburgh Instruments Photoluminescence spectrometer for automated high-throughput measurements of spectra, phosphorescence lifetimes and fluorescence lifetimes with photon-counting sensitivity.

Two options are offered: the PR2 Plate Reader accessory for the FLS1000 Photoluminescence Spectrometer and the SC-41 Plate Reader Module for the FS5 Spectrofluorometer. Both plate readers can utilise the spectrometer’s multiple sources and detectors, as well as its monochromator to fine-tune the wavelength and bandwidth.

If the instrument is configured for time-resolved photoluminescence, these measurements are automatically enabled in the plate reader. This, together with world-leading photon counting electronics, makes the PR2 and SC-41 stand out from traditional microplate readers in terms of flexibility and performance.

Plate Reader Operating Principle

The plate readers are connected to the spectrometer excitation and emission light paths via fibres. The Fluoracle^® software allows choosing the source/detector and the type of measurement, spectral or time-resolved.

The user can create and store custom microplate designs in the software. Measurement wells are selected in the plate layout before taking data. Up to 384 wells can be acquired in one automated measurement, enabling rapid high-throughput photoluminescence experiments.

Example of how Multiwell Plate Reader is set up in software. Shows Plate grid and signal rate information.

Measurement setup for a standard 96 well plate in Fluoracle.

PR2 Plate Reader

SC-41 Plate Reader Module

Typical Performance

Typical Performance	PR2	SC-41
REPEATABILITY (96 WELL)	<0.5%	<1.5%
REPEATABILITY (384 WELL)	<5%	<3%
CROSSTALK (96 WELL)	<0.001%	<0.1%
CROSSTALK (384 WELL)	<0.05%	<5%
WELL-TO-WELL REPEATABILITY	<5%	<3%
SENSITIVITY	1 pM fluorescein	n40 pM fluorescein
SPECTRAL RANGE	250 – 900 nm or 400 – 2,000 nm (fibre dependent)	Excitation 250 – 900 nm Emission 250 – 1,650 nm
DIMENSIONS	401 x 320 x135 mm	320 x 245 x 265 mm (internal to FS5)

Application Notes

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