A fluorescence microplate reader accessory can be added to an Edinburgh Instruments Photoluminescence spectrometer for automated high-throughput measurements of spectra, phosphorescence lifetimes and fluorescence lifetimes with photon-counting sensitivity.
Two options are offered: the PR2 Plate Reader accessory for the FLS1000 Photoluminescence Spectrometer and the SC-41 Plate Reader Module for the FS5 Spectrofluorometer. Both plate readers can utilise the spectrometer’s multiple sources and detectors, as well as its monochromator to fine-tune the wavelength and bandwidth.
If the instrument is configured for time-resolved photoluminescence, these measurements are automatically enabled in the plate reader. This, together with world-leading photon counting electronics, makes the PR2 and SC-41 stand out from traditional microplate readers in terms of flexibility and performance.
Plate Reader Operating Principle
The plate readers are connected to the spectrometer excitation and emission light paths via fibres. The Fluoracle® software allows choosing the source/detector and the type of measurement, spectral or time-resolved.
The user can create and store custom microplate designs in the software. Measurement wells are selected in the plate layout before taking data. Up to 384 wells can be acquired in one automated measurement, enabling rapid high-throughput photoluminescence experiments.
Measurement setup for a standard 96 well plate in Fluoracle.