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MicroPL

Spectromicroscopy Upgrade

Transform your fluorescence spectrometer into a combined spectrometer and microscope system with the MicroPL upgrade for FLS1000 and FS5 instruments.

MicroPL

Spectromicroscopy Upgrade

  • Plug and Play : Easy integration with FLS1000 Photoluminescence Spectrometer or FS5 Spectrofluorometer​ 
  • Spectral and Time-Resolved: Suitable for any type of fluorescence microscopy experiment​ 
  • Widefield and/or Point Excitation: Study a wide area or a µm spot on the sample ​ 
  • FLIM and PLIM: Fluorescence and/or Phosphorescence Lifetime Imaging Microscopy add-on​ 
  • Advanced Mapping Features: 3D map and surface map for imaging uneven surfaces​ 
  • All-In-One Software: FluoracleMap for image acquisition, analysis and presentation 
  • Antibunching Upgrade: Identify single photo emitters with a Hanbury-Brown-Twiss set up

Live Video Demonstration

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PRODUCT DESCRIPTION

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The MicroPL Fluorescence Microscopy Upgrade for Edinburgh Instruments Spectrometers such as the FLS1000 or FS5 allows the study of spectral or time-resolved photoluminescence of samples in the microscopic scale.​ 

An Edinburgh Instruments photoluminescence (PL) spectrometer is converted into a combined spectrometer and microscope system with this user-friendly upgrade. 

A wide range of microscope configurations, source coupling and detector options are available enabling both steady state and fluorescence lifetime microscopy, as well as automated maps, using the same setup. 

It is also possible to couple your own microscope to the FLS1000 or FS5 for a custom MicroPL upgrade (please get in touch for models supported). 

PRODUCT VIDEO

PRODUCT DETAILS

SOFTWARE

Every MicroPL is equipped with a computer-controlled XYZ stage includes the FluoracleMap software package. This software add-on allows acquiring 2D, 3D and surface maps of photoluminescence lifetimes, as well as complex data analysis such as multiexponential decay fitting and a wide range of map display options.

MEASUREMENT EXAMPLES

Widefield Excitation
Point Excitation
FLIM Add-On
Widefield Excitation

Widefield excitation uses a liquid light guide coupling to the spectrometer to excite the full field of view of the objective.

Point Excitation

Point excitation can be performed with either CW or pulsed lasers for spectral or lifetime PL measurements. MicroPL is compatible with Edinburgh Instruments EPL, HPL, and VPL diode lasers. A spot size of ~2 μm (source and objective dependent) is achievable, enabling to resolve PL spectra and lifetimes in the micrometer scale.​

Both Time-Correlated Single Photon Counting (TCSPC) and Multi-Channel Scaling (MCS) operating modes are compatible, covering a wide range of photoluminescence lifetimes, from a few ps up to seconds. Non-standard applications such as upconversion PL microscopy are possible with the appropriate choice of filters and laser coupling.

FLIM Add-On

MicroPL can be configured with a computer-controlled XYZ stage for Fluorescence Lifetime Imaging Microscopy (FLIM). The FLIM add-on unlocks special features in the Fluoracle software (FluoracleMap) including control of the stage and advanced analysis options for maps, such as multi-component decay fitting algorithms. No additional software packages are required to obtain and analyse data.​

FLIM of Convallaria

TECHNICAL SPECIFICATIONS

Technical Specifications
Technical Specifications
Specifications
Microscope ModelsUpright: Nikon NiU, Olympus BX53
Inverted: Nikon Ti2U
Excitation/Emission Range360 nm - 850 nm (std)
Can be extended with non-standard UV and NIR objectives
Excitation ModesWidefield: Tunable continuous source from spectrometer (steady state)
Point: EPL/HPL/VPL pulsed lasers (TCSPC or MCS lifetimes) and CW lasers (steady state)
Detection ModesMicro-PL spectroscopy with spectrometer
Fluorescence Microscopy with additional camera
Objective MagnificationOptions available from 5X to 100X
Sample StageManual or PC-controlled XYZ stage with specifications:
75 mm x 50 mm or 130 mm x 85 mm options
XY resolution 0.01 µm
Z resolution 0.002 µm
SoftwareMapping features in Fluoracle included with PC-controlled XYZ stage
AccessoriesSoftware-controlled cryostat upgrade

APPLICATION NOTES

  • Technical Note: Fluorescence Microscopy with the FS5 Spectrofluorometer
All Application Notes

DOWNLOADS

  • MicroPL Datasheet
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