Time-Resolved Fluorescence

Time-Resolved Fluorescence (or Fluorescence Lifetime) Spectroscopy is an extension of Steady State Fluorescence. Fluorescence lifetimes, occurring as emissive decays from the singlet-state, can also be approximated as those decays occurring in the time region from picoseconds to nanoseconds.

When we discuss time-resolved fluorescence or fluorescence lifetimes, what we are studying is the fluorescence of a sample monitored as a function of time after excitation by a pulse of light.

  • Single and Multiple Exponential Decays
  • Time-Resolved Emission Spectroscopy (TRES)
  • Monomer-Excimer Kinetics
  • Time-Resolved Fluorescence Anisotropy
  • Solvent Relaxation Dynamics
Ilaria Angeloni et al.2017 Band-Edge Oscillator Strength of Colloidal CdSe/CdS Dot-in-Rods: Comparison of Absorption and Time-Resolved Fluorescence SpectroscopyNanoscale
Anna Gakamsky et al.2017 Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular LevelScientific Reports740375
Jinhyung Park et al.2016 Efficient eco-friendly inverted quantum dot sensitized solar cellsJournal of Materials Chemistry A4827-837
Sagar Kesarkar et al.2016 Near-IR Emitting Iridium(III) Complexes with Heteroaromatic β-Diketonate Ancillary Ligands for Efficient Solution-Processed OLEDs: Structure–Property CorrelationsAngewandte Chemie1282764-2768
Haiping He et al.2016 Exciton localization in solution-processed organolead trihalide perovskitesNature Communications710896
Cédric Mongin et al.2016 Direct observation of triplet energy transfer from semiconductor nanocrystalsScience351369-372
V. Caligiuri et al.2016 Dielectric singularity in hyperbolic metamaterials: the inversion point of coexisting anisotropiesNature Scientific Reports620002
Xixi Qin et al.2016 Hybrid coordination-network-engineering for bridging cascaded channels to activate long persistent phosphorescence in the second biological windowNature Scientific Reports620275
Yanyan Li et al.2014 A Single-Component White-Emitting CaSr2Al2O6:Ce3+, Li+, Mn2+ Phosphor via Energy TransferInorganic Chemistry537668-7675
Chen Liao et. al2015 Bright white-light emission from Ag/SiO2/CdS-ZnS core/shell/shell plasmon couplersNanoscale
Choi, M. K. et al.2015 Wearable red-green-blue quantum dot light-emitting diode array using high-resolution intaglio transfer printingNature Communications67149
Zhang, Zuolun et al.2015 D–π–A triarylboron compounds with tunable push–pull character achieved by modification of both the donor and acceptor moietiesChemistry - A European Journal21177-190
De-Chao Yu et al.2015 Multi-photon quantum cutting in Gd2O2S:Tm3+ to enhance the photo-response of solar cellsLight: Science & Applications4e344
Jamie C. Wang et al.2015 Modulating Electron Transfer Dynamics at Dye–Semiconductor Interfaces via Self-Assembled BilayersThe Journal of Physical Chemistry C119(7)3502-3508
Catherine E. McCusker et al.2014 Excited State Equilibrium Induced Lifetime Extension in a Dinuclear Platinum(II) ComplexJ. Phys. Chem. A118 (45)10391-10399
Raju Laishram et. al2015 White light emitting soft materials from off-the-shelf ingredientsJournal of Materials Chemistry C235
Nadia Anikeeva et al.2012 Evidence that the Density of Self Peptide-MHC Ligands Regulates T-Cell Receptor SignalingPLOS One7e41466

The time-resolution can be obtained in a number of ways, depending on the required sensitivity and time regions. Edinburgh Instruments employs the technique called Time-Correlated Single Photon Counting (TCSPC), for Time-Resolved Fluorescence, which is used for the acquisition of single photons and allows for time resolutions in the range of picoseconds (ps) to nanoseconds (ns).

time-domain decayThis techniques is a digital counting technique, counting photons that are time-correlated in relation to a short excitation light pulse.

In TCSPC the sample is repetitively excited using a pulsed light source with a high repetition rate. During the measurement a probability histogram builds, which relates the time between an excitation pulse (START) and the observation of the first fluorescence photon (STOP).

The fact that the time at which a fluorescence photon is incident on the detector can be defined with picosecond resolution is critical to the operation and precision of TCSPC.

To study lifetime decays slower than this (ns to seconds time range) please see Phosphorescence Lifetime.

To find out more about what TCSPC is and why we use TCSPC, please see the technical notes provided in the resources section.

To talk to a member of our Sales Team regarding time resolved fluorescence products, email them at or use the Enquiry Form in the tab above.