Biology/Life Science Archives | Edinburgh Instruments

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Application Note: Raman Microscopy for Pharmaceutical Analysis

This application note, Raman Microscopy for Pharmaceutical Analysis, gives a complete overview of the pharmaceutical applications of the RM5 and RMS1000 Confocal Raman Microscopes.


Application Note: Discrimination of Bacteria Species Using Raman Microscopy and Principal Component Analysis

In this Application Note, Raman spectroscopy is coupled with principal component analysis (PCA) to discriminate between bacterial species.


Application Note: Batch and Global Analysis of Fluorescence Lifetimes

This application note shows the potential application of a plate reader on an FLS1000 and fluorescence lifetime analysis using batch analysis and global analysis.


Application Note: Detection of Singlet Oxygen by Photoluminescence Spectroscopy

In this application note, an FLS1000 Photoluminescence Spectrometer is used to characterise the PL spectrum and lifetime of singlet oxygen in solution. Different excitation sources and detectors are compared to determine the best FLS1000 configuration for singlet oxygen detection.


Application Note: Temperature-Dependent Quantum Yield of Chlorophyll Fluorescence in Plant Leaves

Fluorescence spectroscopy is a powerful tool to investigate the photochemistry of biological systems. In this application note the influence of temperature on the photochemistry of perennial leaves is investigated by measuring the fluorescence quantum yield over a temperature range of 77 K to 300 K using the FLS1000 equipped with the Cryosphere accessory.


Application Note: Study of Fluorescence Quenching Kinetics Using Stopped-Flow

A powerful method for determining the kinetics of a reaction is by monitoring the concentration of the reactants or products over time using stopped-flow fluorescence spectroscopy. In this application note, the kinetics of NATA quenching by QBS are determined using the FLS1000 Photoluminescence Spectrometer equipped with the stopped-flow accessory.


Application Note: ATR-FTIR of Blood Serum Using a Heated ATR Accessory

This application note demonstrates the use of ATR-FTIR spectroscopy for the analysis of human serum albumin. An IR5 FTIR spectrometer was used, equipped with a heated ATR accessory, to help tackle the issue of strong water absorption bands masking the IR bio-spectrum.


Application Note: Kinetics of Persistent Luminescence Phosphors

Persistent luminescence, commonly called afterglow, is long-lasting visible emission over several hours after ultraviolet excitation. It has many applications; ranging from glow-in-the-dark signage to in-vivo imaging for disease diagnosis and treatment. In this application note, the persistent luminescence kinetics of a doped strontium aluminate phosphor are characterised using the FLS1000 Photoluminescence spectrometer


Application Note: Multiphoton Imaging of Mouse Intestine

In this application note, an RMS1000 Confocal Microscope is used to image a tissue section of mouse intestine using two-photon excited fluorescence and second harmonic generation microscopy.


Application Note: Molecular Beacon Probe Fluorescent Detection of DNA

Molecular beacon probes are a sequence of nucleotides (the building blocks of DNA and RNA) that can be used to fluorescently detect the presence of a specific sequence of DNA or RNA. With real-world examples as PCR quantification, in vivo RNA detection, pathogen detection and viral load quantification. The use of molecular beacons, coupled with a sensitive spectrofluorometer facilitates the measurement of extremely low concentrations of DNA or RNA. In this application note, nanomolar concentrations of cDNA were quantified using a molecular beacon while controlling the temperature of incubation and measuring the sample emission with an Edinburgh Instruments FS5 Spectrofluorometer.