DS5 Dual Beam UV-Vis Spectrophotometer | Edinburgh Instruments
DS5 Dual Beam UV-Vis Spectrophotometer

DS5 Dual Beam UV-Vis Spectrophotometer

  • User selectable variable bandpass options at 0.5, 1.0, 1.5, 2.0 or 4.0 nm
  • Fast scanning – up to 6,000 nm/min to aid sample analysis throughput
  • Automatic accessory recognition connected to the DS5
  • Modern touchscreen interface with intuitive menus and functions to ensure ease of use for standalone control
  • USB, SD card and internal data storage for convenient retrieval of methods and results
  • Compact footprint to maximise available bench space in the laboratory

Product Description

DS5 Dual Beam UV-Vis Spectrophotometer

The DS5 UV-Vis Spectrophotometer is a high performance dual beam instrument suitable for many analytical applications where accuracy and precision measurements are key to your results.

Developed and designed in the UK, the DS5 provides a modern, user-friendly and accurate spectrophotometer for a wide range of sample types and measurements.

Utilising a dual lamp and Czerny-Turner configuration monochromator, the DS5 features a compact, reliable and high throughput optical system which ensures impressive spectral performance. Additional benefits include stray light, baseline flatness, wavelength and photometric accuracy and reproducibility.

For further information, or to find out how our UV-Vis spectrophotometer can assist you with your research, simply contact a member of our sales team at sales@edinst.com.

Technical Specifications

Parameter Description
Optics Czerny-Turner, Dual Beam Monochromator
Wavelength Range 190 nm – 1100 nm
Spectral Bandwidth 0.5 nm, 1 nm, 1.5 nm, 2 nm and 4 nm
Stray Light ≤0.10% T (220 nm NaI, 340 nm NaNO2)
Wavelength Accuracy ±0.1 nm
Wavelength Repeatability ±0.1 nm
Photometric Range Absorbance: -3.4 to +3.4, %T: 0 to 300, Concentration: 0,000 to 9,999
Photometric Accuracy ±0.002 Abs (0 – 0.5 Abs), ±0.004 Abs (0.5 – 1.0 Abs), ±0.008 Abs (1.0 – 2.0 Abs)
Photometric Reproducibility ±0.0003 Abs (0-0.5 Abs), ±0.0005 Abs (0.5-1 Abs), ±0.001 Abs (1.0-2 Abs)
Wavelength Scan Speed 10, 100, 200, 400, 800, 1200, 2400, 3600, 6000 nm/min
RMS Noise ≤0.00003 Abs (0 Abs, 500nm)
Baseline Stability <0.0003 Abs/hr (500 nm, 2 hour lamp warm-up period)
Baseline Flatness ±0.0009 Abs (200 nm – 950 nm)
Light Source Tungsten-Halogen and Deuterium Lamps
Light Source Switching Automatic switching selectable for 325 nm – 370 nm range
Detector Silicon Photodiode
Display 7” Touchscreen
Software Touchscreen operation, PC-controlled software or CFR compliant PC-controlled software
Dimensions 500 mm (W) × 475 mm (D) × 250 mm (H)
Net Weight 20 Kg (approx)
Power Supply 100 – 240V, 50/60 Hz, 150VA
Ambient Temperature 10°C – 35°C
Output Device USB flash drive, SD card
Interface USB PC interface


Home Screen Measurement Setup
DS5 Software Home Screen DS5 Measurement Software

Measurement Modes

Photometry Mode: Perform quantitative analyses in either absorbance or transmittance modes. Select from single up to 5 different individual wavelengths, nucleic acid/protein A260/A280 ratios and set up calibration curves for concentration measurements.

Time Scan: Perform kinetic measurements for time periods ranging from 1 minute to >27 hours. Measurement intervals are factory preset and automatically selected when the scan time is set.

Wavelength scan: Perform a full spectral scan from 190 nm – 1100 nm at any of 9 incremental and preset selectable scan speeds starting from a high resolution 10 nm/min up to a maximum scan speed of 6,000 nm/min. Data is displayed as a graphical spectrum on which data analysis can be performed through the touchscreen interface with zoom, peak-valley, smoothing and other functions available through the easy to use menu interface.

  • Concentration measurement
  • Absorbance/Transmittance measurement
  • Hexavalent Chromium, Nucleic acid measurement (nucleic acid purity, nucleic acid concentration, protein concentration calculation)
  • Wavelength scan
  • Time change
  • Single and multi-wavelength photometry
Spectrum Overlay Area Calculation
Spectrum Overlay - DS% Spectrophotometer Area Calculation DS5 Spectrophotometer

Validation Functions

To ensure optimum instrument performance, self-diagnosis incorporating a number of parameters and wavelength calibration are automatically initiated upon start-up. Furthermore, the DS5 is equipped with a GLP/GMP feature for analyses requiring validation and auditing. Parameters such as wavelength accuracy, wavelength reproducibility, bandpass, baseline flatness, baseline stability and noise level can all be validated.

Data Handling

  • Scale changing, Trace, Spectrum overlay, Peak & trough detection, Smoothing, Differentiation, and Area & rate calculating
  • Internal memory, External USB, SD card
  • File output in CSV format