Technical Notes Archives | Edinburgh Instruments

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Guide for the Measurements of Absolute Quantum Yields of Liquid Samples

Fluorescence Quantum Yields can be measured using the relative method by comparing fluorescence parameters to a published quantum yield standard, or they can be determined by the absolute method in which the number of absorbed photons of a sample and the number of consequently emitted photons are measured.This Guide is designed to help you to determine the Fluorescence Quantum Yields (QY) of liquid samples using your FLS920 spectrometer and the Integrating Sphere accessory.
FLS1000 FS5

Reflection, Absorption, Quantum Yield Measurements of Powder Samples Using the Integrating Sphere

Accurate Quantum Yield Measurements as well as precise measurements of the spectral dependence of reflection and/or absorption of powder samples, require an integrating sphere. Edinburgh Instruments offers a demountable integrating sphere for quantum yield and reflection measurements of solutions or solid samples. The integrating sphere fits directly into the sample chamber and is supplied with a cuvette holder and a mount for powder samples.
FLS1000 FS5

Transmission and Absorbance of Liquid Samples

The absorbance or optical density of a substance is inherent in all aspects of spectroscopy and follows the Beer-Lambert law. In this Technical Note, our FS5 Spectrofluorometer is equipped with a silicon detector that allows for transmission and absorbance measurements. After the transmission scans of the sample and reference are complete, they can be joined to calculate the absorbance through the absorption wizard in our operating software, Fluoracle.
FLS1000 FS5

Quenching of Fluorescence with Temperature

Reliable fluorescence standards and stable fluorescent probes for bio-analytics and chemistry are as important as sensitive indicator fluorophores that utilise the outstanding property of fluorescence of being highly sensitive to the fluorophore’s micro-environment. In this technical note we use the FS5 Spectrofluorometer to show the quenching of fluorescence with temperature. By using the extended capabilities of FS5-TCSPC we also record, for the same set of temperatures, the fluorescence lifetimes. We can show that temperature dependence of Rhodamine B is exclusively caused by dynamic quenching.

Chromaticity Coordinates of Microcrystalline Phosphors

Luminescent materials used in lighting are standardized by the International Commission on Illumination (CIE). This standardisation is important for the lighting industry and is based on the illuminating conditions, the brightness and the observer. In this technical note, a series of phosphors emitting across the visible range were characterised in an FS5 Spectrofluorometer and their chromaticity coordinates calculated in its integrated software package, Fluoracle®.
FLS1000 FS5