Biology/Life Science Archives | Edinburgh Instruments

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Proton-Coupled Electron Transfer (PCET); Spectral and Kinetic Transient Absorption Analysis of Acridine Orange with Tri-Tert-Butylphenol

Understanding fundamental photo-induced proton-coupled electron transfer (PCET) reactions of small molecules is central to research ranging from solar fuels, biological signalling, and organic electronics. By combining spectral and kinetic information of the photo-generated excited states and radical species present, researchers are able to elucidate the PCET reaction mechanisms involved in these applications. The Edinburgh Instruments LP Transient Absorption Spectrometer is the world’s only commercial system capable of recording instantaneous spectral information utilising an ICCD camera, while then easily being able to switch to measuring kinetic data on a PMT detector – all under software control. It was used to carry out the research in this application note.

Application Note: Kinetics of Persistent Luminescence Phosphors

Persistent luminescence, commonly called afterglow, is long-lasting visible emission over several hours after ultraviolet excitation. It has many applications; ranging from glow-in-the-dark signage to in-vivo imaging for disease diagnosis and treatment. In this application note, the persistent luminescence kinetics of a doped strontium aluminate phosphor are characterised using the FLS1000 Photoluminescence spectrometer
FLS1000 FS5 LP980

Time-Resolved Fluorescence Techniques to FRET Based Immunoassay

Förster resonance energy transfer (FRET) is a phenomenon of non-radiative transfer of the optical excitation from an excited electronic level of one molecule, called the donor, to a resonant electronic level of another molecule, called the acceptor. In this application note we show that one can increase the method sensitivity and reduce cost of the assay by applying a time-resolved fluorescence format to the FRET-based immunoassay.
FLS980 FLS1000 FS5