Resources

This application note the near-infrared (NIR) detection capabilities of the FS5 are employed to examine quantum dots (QDs). Solvent choice in this region can interfere with emission of the sample.

One of the most powerful techniques to understand the behaviour and performance of electrical devices is electroluminescence spectroscopy. In this application note the FLS1000 Photoluminescence Spectrometer is used to investigate the emission properties of a phosphor coated indium gallium nitride white light LED using both steady state and time-resolved electroluminescence spectroscopy.

Perovskite photovoltaic cells are exposed to a wide range of temperatures and it is therefore important to understand the influence of temperature on the properties of the perovskite. In this application note, the FLS1000 spectrometer equipped with the liquid nitrogen cryostat accessory is used to determine the phase transition temperatures of MAPI perovskite.

A powerful method for determining the kinetics of a reaction is by monitoring the concentration of the reactants or products over time using stopped-flow fluorescence spectroscopy. In this application note, the kinetics of NATA quenching by QBS are determined using the FLS1000 Photoluminescence Spectrometer equipped with the stopped-flow accessory.

Persistent luminescence, commonly called afterglow, is long-lasting visible emission over several hours after ultraviolet excitation. It has many applications; ranging from glow-in-the-dark signage to in-vivo imaging for disease diagnosis and treatment. In this application note, the persistent luminescence kinetics of a doped strontium aluminate phosphor are characterised using the FLS1000 Photoluminescence spectrometer

Optical probes based on lanthanide complexes are of particular interest in the biomedical field and life sciences due to their unique magnetic and photoluminescent properties. In this application note, an Edinburgh Instruments FS5 Spectrofluorometer is used for a complete characterisation of the lanthanide-based optical probes.

Molecular beacon probes are a sequence of nucleotides (the building blocks of DNA and RNA) that can be used to fluorescently detect the presence of a specific sequence of DNA or RNA. With real-world examples as PCR quantification, in vivo RNA detection, pathogen detection and viral load quantification. The use of molecular beacons, coupled with a sensitive spectrofluorometer facilitates the measurement of extremely low concentrations of DNA or RNA. In this application note, nanomolar concentrations of cDNA were quantified using a molecular beacon while controlling the temperature of incubation and measuring the sample emission with an Edinburgh Instruments FS5 Spectrofluorometer.

Quantum yield is a fundamental photophysical parameter that describes a sample's fluorescence efficiency, and it can be measured optically via the absolute method and relative method. 2-Aminopyridine (2AMP) in sulfuric acid (H2SO4) has been previously used as a quantum yield reference standard in the UV-visible range. However, the 2AMP quantum yield values are now decades old. In this application note, we present a reinvestigation and revaluation of the quantum yield of 2AMP in 1M H2SO4, using quinine bisulphate (QBS) in 1M H2SO4 as the reference standard with an Edinburgh Instruments FS5 Spectrofluorometer.

This customer written application note describes how to measure the two-photon absorption spectra of fluorescent chromophores by using an external short-pulsed laser source in an Edinburgh Instruments FS5 Spectrofluorometer. A procedure on how to calculate the nonlinear refraction dispersion from the measured spectra is also given.

Semiconductor quantum dots (QDs) have unique tuneable photoluminescence properties which lend them to a range of important technological applications including solid-state lighting, displays, photovoltaics, and biomedical imaging. Indium phosphide (InP) QDs have attracted significant interest as an environmentally friendly and non-toxic alternative to traditional heavy metal based QDs containing cadmium and lead.