Transmission and Absorbance of Liquid Samples
The absorbance or optical density of a substance is inherent in all aspects of spectroscopy and follows the Beer-Lambert law. In this Technical Note, our FS5 Spectrofluorometer is equipped with a silicon detector that allows for transmission and absorbance measurements. After the transmission scans of the sample and reference are complete, they can be joined to calculate the absorbance through the absorption wizard in our operating software, Fluoracle.
