When fluorescence spectra are measured in Edinburgh Instruments fluorescence spectrometers, two types of correction can be applied to the spectra; excitation and emission. In our previous blog post, we focused on emission correction and its role in the detection system. Now we turn our attention to excitation correction.
Frequently Asked Questions
The Jablonski Diagram is widely used in fluorescence spectroscopy to illustrate the excited states of a molecule and the radiative and non-radiative transitions that can occur between them.
The terms; luminescence, photoluminescence, fluorescence and phosphorescence are routinely used in research papers to describe the emission of light from a sample. This multiple naming can be confusing with several of the terms being used interchangeably and researchers from different scientific backgrounds preferring to use one name over another. In this article we answer the question, “What is the Difference between Luminescence, Photoluminescence, Fluorescence, and Phosphorescence”.
Time-correlated single-photon counting (TCSPC) is a well established and common technique for fluorescence lifetime measurements, it is also becoming increasingly important for photon migration measurements, optical time domain reflectometry measurements and time of flight measurements.
Fluorescence spectroscopy is a widespread analytical technique, but dealing with unknown samples can often be challenging even for experienced users. Our team have put together a procedure for spectral measurements to help newcomers in the fluorescence spectroscopy field.
Acquiring and interpreting steady state fluorescence data is not always straightforward. Below we offer some advice on commonly encountered problems to help you optimise the operation of your Edinburgh Instruments spectrometer.
The inner filter effect is a common problem in fluorescence spectroscopy, affecting spectral measurements in particular. Our team has come up with a solution to this.
Our team have put together a description on the inner workings of fluorescence spectrometers, and have provided a basic guide on fluorescence.