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Quenching of Fluorescence with Temperature

Reliable fluorescence standards and stable fluorescent probes for bio-analytics and chemistry are as important as sensitive indicator fluorophores that utilise the outstanding property of fluorescence of being highly sensitive to the fluorophore’s micro-environment. In this technical note we use the FS5 Spectrofluorometer to show the quenching of fluorescence with temperature. By using the extended capabilities of FS5-TCSPC we also record, for the same set of temperatures, the fluorescence lifetimes. We can show that temperature dependence of Rhodamine B is exclusively caused by dynamic quenching.

Chromaticity Coordinates of Microcrystalline Phosphors

Luminescent materials used in lighting are standardized by the International Commission on Illumination (CIE). This standardisation is important for the lighting industry and is based on the illuminating conditions, the brightness and the observer. In this technical note, a series of phosphors emitting across the visible range were characterised in an FS5 Spectrofluorometer and their chromaticity coordinates calculated in its integrated software package, Fluoracle®.
FLS1000 FS5

Technical Note: An Introduction to Flash Photolysis using the LP980 Spectrometer

In flash photolysis, a short pulse of light generates a transient (temporary) species in a sample; e.g. a radical intermediate or a short-lived excited state. As the sample relaxes back to the ground state or the final reaction products, the changes occurring are recorded as a function of time using a spectroscopic technique. A flash photolysis spectrometer typically monitors the change in absorption as a function of time, utilising a second light source (probe) besides the excitation (pump) pulse going through the sample. This technique is also known as transient absorption (TA). This technical note is aimed as an introduction to nanosecond flash photolysis for newcomers to the technique. We describe the operation of the LP980 spectrometer and show examples of measurements, with references to real-life applications.