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Guide for the Measurements of Absolute Quantum Yields of Liquid Samples

Fluorescence Quantum Yields can be measured using the relative method by comparing fluorescence parameters to a published quantum yield standard, or they can be determined by the absolute method in which the number of absorbed photons of a sample and the number of consequently emitted photons are measured.This Guide is designed to help you to determine the Fluorescence Quantum Yields (QY) of liquid samples using your FLS920 spectrometer and the Integrating Sphere accessory.
FLS1000 FS5

Reflection, Absorption, Quantum Yield Measurements of Powder Samples Using the Integrating Sphere

Accurate Quantum Yield Measurements as well as precise measurements of the spectral dependence of reflection and/or absorption of powder samples, require an integrating sphere. Edinburgh Instruments offers a demountable integrating sphere for quantum yield and reflection measurements of solutions or solid samples. The integrating sphere fits directly into the sample chamber and is supplied with a cuvette holder and a mount for powder samples.
FLS1000 FS5

Transmission and Absorbance of Liquid Samples

The absorbance or optical density of a substance is inherent in all aspects of spectroscopy and follows the Beer-Lambert law. In this Technical Note, our FS5 Spectrofluorometer is equipped with a silicon detector that allows for transmission and absorbance measurements. After the transmission scans of the sample and reference are complete, they can be joined to calculate the absorbance through the absorption wizard in our operating software, Fluoracle.
FLS1000 FS5