Detection of Singlet Oxygen is of particular interest in a multitude of applications such as photodynamic treatment of cancer1, fine chemical synthesis2 and treatment of wastewater.3
1O2 shows a weak emission band at ~1270 nm with a natural bandwidth of approximately 18 nm. As the ground state of molecular oxygen is a triplet state, direct excitation, by the absorption of light, into the first excited singlet state is not possible. Therefore 1O2 is often generated by chemical reactions or by photosensitisation, i.e. light is absorbed by other photoactive dyes that will be able to transfer the absorbed energy via their triplet states to the molecular oxygen.
As the signal at 1270 nm is so weak, detection of the 1O2 emission has always been challenging. Germanium and InGaAs detectors were used in the past, but predominantly for steady state applications. Lifetime applications were very limited indeed, due to the further reduction of signal amplitude when the RC constant of the detector had to be kept low to achieve the necessary temporal resolution. Nowadays, photomultipliers (PMT) are available with sensitivity to 1400 nm and beyond. This greatly facilitates both steady state and lifetime measurements of 1O2.
Even with modern detectors, it is essential to have a powerful excitation source and an efficient optical system. The FLS980 is the ideal spectrometer for this application.
Emission spectra were measured in a FLS980 Fluorescence Spectrometer equipped with a 450 W Xe lamp, a 60 W microsecond flashlamp, and single monochromators. A 400 nm blazed grating was used at the excitation and a 1200 nm blazed grating at the emission arm.
An NIR-PMT (Hamamatsu, R5509-72) with 0.5 s, 4 repeats and a two-stage TEcooled InGaAs detector (Hamamatsu, G8605-23) with 0.1 s dwell and 20 repeats were used, both resulting in 2 s integration time. Higher diffraction orders were filtered by the built-in long wave-pass filters in the FLS980.
Samples of Erythrosine B (Sigma-Aldrich, 19,826-9) dissolved in anhydrous ethanol (Sigma-Aldrich, 459836) and tris(2,2’-bipyridyl)dichlororuthenium(II) hexahydrate Ru(bpy)3 (Sigma-Aldrich, 544981) dissolved in D2O (Sigma-Aldrich,151882) were prepared air saturated with optical density (OD) 0.5 and 0.35, respectively. The Ru(bpy)3 sample was additionally saturated with oxygen for approximately 10 minutes.
All samples were measured in quartz cuvettes of 10 mm path-length in right angle geometry. The Ru(bpy)3 samples were excited at the absorption peak of 450 nm, while the Erythrosine B at 530 nm with excitation and emission bandwidths of 15 nm.
To allow for direct comparison of the spectra between different detectors, the data were noise normalised by applying a scaling equal to the noise ratio at 1360 nm – 1400 nm, that is f=INIR-PMT(λ)/IInGaAs(λ), and consequently offset vertically.
The 1O2 emission spectra displayed in Figure 1 were measured with a) an NIRPMT and b) an InGaAs detector. The 1O2 emission around 1270 nm generated by erythrosin shows a single emission peak, however in Ru(bpy)3 the 1O2 emission is overlapped by the emission tail of the sensitizer molecule. It can also be seen in Figure 1b that, for the O2 saturated sample, the background emission is reduced due to the quenching of the triplet state4 , as has been observed in Ru(bpy)3 in aqueous solutions.5
The higher source of noise inherent in an analogue detector means that the NIR-PMT will have a higher SNR and therefore be more sensitive at 1270 nm, even though the InGaAs may have higher quantum efficiency.
In order to obtain a direct comparison of the 1O2 signal obtained by the two detectors, the emission spectra of 1O2 from erythrosin B in Figure 1 have been noise normalised and are displayed in Figure 2.
Although the emission spectrum of 1O2 can be measured with InGaAs detectors, the measurement of its lifetime requires a photon counting detector. For this reason, the decay of 1O2 monitored at 1270 nm was measured with an NIR-PMT and is displayed in Figure 3. It can be seen that the exponential fit resulted in a lifetime of 15.3 μs for 1O2 from Erythrosin B in ethanol and 59.47 μs for Ru(bpy)3 in D2O, in agreement with reported decay values.6
The time-resolved emission spectra (TRES) can be obtained from time-resolved scans across the emission of 1O2. In this way, the emission of 1O2 can be distinguished from phosphorescence emitted by the sensitiser. The emission spectra generated by Ru(bpy)3 are displayed in Figure 4 from 200 μs to 400 μs, with a step of 20 μs.
Steady-state and time-resolved emission spectra of 1O2 generated by Erythrosin B and Ru(bpy)3 are reported in this technical note. In addition, the lifetimes were fitted in single exponentials and are in good agreement with reported lifetime values. It can be concluded that both steady-state and time-resolved measurements of 1O2 can be obtained with single photon counting detectors such as NIR-PMT, while InGaAs detectors can be used for steady-state measurements.
Emission spectra were measured using an FLS980 Fluorescence Spectrometer. Our FLS980 has now been discontinued but we are still able to offer upgrades to those with existing systems. Our FLS1000 Photoluminescence Spectrometer has replaced the FLS980, and sets the standard in both steady state and time-resolved photoluminescence spectroscopy for fundamental research and routine laboratory applications. To find out about upgrades for an existing FLS980 system, or to enquire about our FLS1000, contact a member of our sales team at firstname.lastname@example.org.
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