Frequently Asked Questions | Edinburgh Instruments | Blog
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Frequently Asked Questions

What is Quantum Yield?

What is Quantum Yield? In this article we give a useful insight to the history of quantum yield and how to measure it using the Absolute and Relative methods.
Frequently Asked Questions

How to choose your lasers for Raman spectroscopy

Which lasers do you need for Raman spectroscopy? Exploring the positives and negatives of the three laser regions used in Raman spectroscopy: UV, visible, and near-infrared, we review the considerations to be made for optimising a Raman system to your application needs!
Frequently Asked Questions

What is Upconversion?

Photon upconversion is the sequential absorption of two or more long wavelength photons leading to the emission of a photon with a shorter wavelength.
Frequently Asked Questions

What is a Spectrometer?

In the broadest sense a spectrometer is any instrument that is used to measure the variation of a physical characteristic over a given range; i.e. a spectrum. The most ubiquitous type of spectrometer used for research are optical spectrometers; and when someone simply says ‘spectrometer’, without an additional qualifier, they are usually referring to an optical spectrometer and this diverse
Frequently Asked Questions

What is the Stokes Shift?

Stokes Shift, named after Irish physicist George Gabriel Stokes, is the term given to the spectral shift to lower energy between the incident light and the scattered or emitted light after interaction with a sample. The Stokes Shift is an important concept in both Fluorescence and Raman spectroscopy and is introduced in this article.
Frequently Asked Questions

What are Absorption, Excitation and Emission Spectra?

Learn more about the differences between molecular absorption, excitation and emission spectra in this latest blog post.
Frequently Asked Questions

Excitation Correction in a Fluorescence Spectrometer

When fluorescence spectra are measured in Edinburgh Instruments fluorescence spectrometers, two types of correction can be applied to the spectra; excitation and emission. In our previous blog post, we focused on emission correction and its role in the detection system. Now we turn our attention to excitation correction.
Frequently Asked Questions

What is a Jablonski Diagram (Perrin-Jablonski Diagram)?

The Jablonski Diagram is widely used in fluorescence spectroscopy to illustrate the excited states of a molecule and the radiative and non-radiative transitions that can occur between them.
Frequently Asked Questions

What is the Difference between Luminescence, Photoluminescence, Fluorescence, and Phosphorescence?

The terms; luminescence, photoluminescence, fluorescence and phosphorescence are routinely used in research papers to describe the emission of light from a sample. This multiple naming can be confusing with several of the terms being used interchangeably and researchers from different scientific backgrounds preferring to use one name over another. In this article we answer the question, "What is the Difference
Frequently Asked Questions

TCSPC - What is Time-Correlated Single Photon Counting?

Time-correlated single-photon counting (TCSPC) is a well established and common technique for fluorescence lifetime measurements, it is also becoming increasingly important for photon migration measurements, optical time domain reflectometry measurements and time of flight measurements.