Frequently Asked Questions | Edinburgh Instruments | Blog
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Infrared or Raman Spectroscopy?

IR and Raman spectroscopy are two complimentary vibrational spectroscopy techniques. How to choose which one to use? Our Spectral School article explains how to tell if your samples are better suited to Raman or IR spectroscopy and why.

What is Fluorescence Lifetime?

Fluorescence lifetime is an important photophysical parameter, providing insights into the energy relaxation and dynamics of the species under study.
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What are Absorption, Excitation and Emission Spectra?

Learn more about the differences between molecular absorption, excitation and emission spectra in this latest blog post.
Frequently Asked Questions

What is Quantum Yield?

What is Quantum Yield? In this article we give a useful insight to the history of quantum yield and how to measure it using the Absolute and Relative methods.
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How to Choose your Lasers for Raman Microscopy

Which lasers do you need for Raman spectroscopy? Exploring the positives and negatives of the three laser regions used in Raman spectroscopy: UV, visible, and near-infrared, we review the considerations to be made for optimising a Raman system to your application needs!
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What is Upconversion?

Photon upconversion is the sequential absorption of two or more long wavelength photons leading to the emission of a photon with a shorter wavelength.
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What is a Spectrometer?

In the broadest sense a spectrometer is any instrument that is used to measure the variation of a physical characteristic over a given range; i.e. a spectrum. The most ubiquitous type of spectrometer used for research are optical spectrometers; and when someone simply says ‘spectrometer’, without an additional qualifier, they are usually referring to an optical spectrometer and this diverse
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What is the Stokes Shift?

Stokes Shift, named after Irish physicist George Gabriel Stokes, is the term given to the spectral shift to lower energy between the incident light and the scattered or emitted light after interaction with a sample. The Stokes Shift is an important concept in both Fluorescence and Raman spectroscopy and is introduced in this article.
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Excitation Correction in a Fluorescence Spectrometer

When fluorescence spectra are measured in Edinburgh Instruments fluorescence spectrometers, two types of correction can be applied to the spectra; excitation and emission. In our previous blog post, we focused on emission correction and its role in the detection system. Now we turn our attention to excitation correction.
Frequently Asked Questions

What is a Jablonski Diagram (Perrin-Jablonski Diagram)?

The Jablonski Diagram is widely used in fluorescence spectroscopy to illustrate the excited states of a molecule and the radiative and non-radiative transitions that can occur between them.