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Integrating Sphere for Quantum Yield Measurements

The Edinburgh Instruments integrating sphere is an accessory for the measurement of fluorescence quantum yields. It is 150mm in diameter and has its inner surface coated with Barium Sulphate (BaSO4) to enable efficient scattering of light over a wide wavelength range.

The sphere has 2 perpendicular ports, one to allow the excitation beam into the sphere and one to collect a portion of the light scattering off the sphere surface and direct it into the emission arm of the spectrometer. It fits directly in the sample chamber of the FLS920 spectrometer series where the regular sample holder is normally placed. The integrating sphere is shown below on the left and the placement within the spectrometer is shown on the right.

With the integrating sphere the measurement of fluorescence quantum yield by an absolute method is possible. A cuvette holder for both direct and indirect excitation is provided to enable correction for re-absorption of scattered excitation light.
 

                             

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