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LifeSpec Red Spectrometer

The LifeSpec-Red is a compact, high performance, computer controlled spectrometer for the rapid measurement of fluorescence lifetimes based on the technique of Time Correlated Single Photon Counting.

Features Include :

  • Fluorescence lifetimes from 30picoseocnds to 10microseconds
  • Spectral range 300-850nm
  • High dynamic range and temporal resolution
  • Time Correlated Single Photon Counting operation
  • PC plug-in card for TCSPC
  • Fully computer controlled through Windows software
  • Computer controlled Time Resolved Emission Spectra acquisition

Full data reconvolution using a non-linear least square fitting routine
The LifeSpec-Red is a compact, high performance, computer controlled spectrometer for the rapid measurement of fluorescence lifetimes based on the technique of Time Correlated Single Photon Counting. The LifeSpec-Red can be flexibly configured with a choice of high repetition rate, short pulse length, excitation sources with selectable wavelength spanning the UV-Visible (picosecond semiconductor diode lasers and pulsed light emitting diodes (LED)). For further details of these sources please refer to table 1 below. The LifeSpec-Red is supplied with a peltier cooled single photon counting sensitive PMT with spectral response 185-850nm.

The LifeSpec Red consists of the following hardware :

  • Choice of short pulsed excitation source (see EPLs below)

  • Sample chamber with liquid cooled cuvette holder and temperature monitor,

  • 100 mm focal length low temporal dispersion, high stray light rejection emission monochromator. Computer controlled wavelength selection, manual slit selection, wavelength accuracy 1nm

  • Single Photon counting sensitive detector wavelength response 300-850nm. Instrumental response function 200ps. Supplied in room temperature housing, with integral HV supply

  • TCC900 card for TCSPC data acquisition

  • Dedicated PC running F900 software for comprehensive spectrometer control, performance monitoring, fluorescence lifetime data acquisition and analysis library
     

EPLs - Edinburgh Picosecond Diode Lasers

The EPL picosecond diode lasers have been designed as a light source for TCSPC systems. They have been pre-adjusted for the best pulsewidth and spectrally purified output. A choice of 10 repetition rates which match the TCSPC electronics of the Lifespec are available.

Picosecond Pulsed Light Sources                            

    EPL 375 EPL 405 EPL 445 EPL 475 EPL 635
Wavelength (±10 nm) [nm] 375 405 445 475 635
Linewidth (nm) [nm] <2.0 <2.0 <2.0 <2.0 <2.0
Max Pulse Width @10MHz [ps] 90 90 100 90 100
Typical Pulse Width @10MHz [ps] 60 70 95 85 95
Typical Average Power @20MHz [mW] 0.080 0.35 0.15 0.15 0.30
Min. Average Power @20MHz [mW] 0.065 0.25 0.10 0.10 0.10
Typical Peak Power @10MHz [mW] 80 350 65 100 90
Min Peak Power @10MHz [mW] 150 150 55 55 55

Pulse Repetition Frequencies for EPLs

Pulse Repetition Frequencies 20 MHz 10 MHz 5 MHz 2 MHz 1 MHz 500 kHz 200 kHz 100 kHz 50 kHz 20 kHz
Pulse period 50 ns 100 ns 200 ns 500 ns 1 ms 2 ms 5 ms 10 ms 20 ms 50 ms

Software Interface  :

The F900 advanced software is a user friendly package providing the user with complete instrument control. The F900 software controls measurement set-up and data acquisition all through a transparent menu. Control of the hardware settings on the TCC900 card are easily accessed through the F900 software. The software also provides the user with decay curve analysis for up to four exponential decay processes.

 

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System Flexibilty  :

The modular design of every spectrometer provides maximum flexibility to meet the requirements for a wide range of applications. We can offer a range of accessories for use with the instrument but we also have off the shelf up-grade routes that are quick and easy to implement.

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Data Acquisition :

The LifeSpec-Red utilises the technique of Time Correlated Single Photon Counting (TCSPC) for fluorescence lifetime data acquisition.

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Datasheet  (2mb)

 
 
 

 

 

 
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