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The Fluorescence
Lifetime Plate Reader will be the
first commercially available single
modality plate reader based on the
technique of Time Correlated Single
Photon Counting.
While
TCSPC has been the established
method of choice for high precision
fluorescence lifetime measurements
in photochemistry for many years,
this technique is now increasingly
used in the field of biological
research. This is because of two
reasons: Firstly, the requirement on
quality and variety of data in
biosciences is dramatically rising,
and secondly the high standard in
electronic integration and
ultra-fast pulsed semiconductor
light sources – fundamental
requirements for system integration
- have only recently become
available.
Edinburgh Instruments have worked on
this project in collaboration with
dye
manufacturing, labelling, drug
developing, and assay developing
companies and institutions. The
system is currently undergoing
stringent validation tests and will
soon be commercially available.
Features include:
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Time Correlated Single Photon
Counting Acquisition times less
than 1second/well
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Temporal resolution
down to picoseconds
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96, 384, 1536 well plates
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Least Squares and Maximum
Likelihood Analysis software
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Four excitation wavelengths
available: 370nm, 405nm, 440nm,
470nm
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Spectral Range of Fluorescence
Detection: 425nm - 670nm
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Full computer control through
Windows software
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User friendly software for
biological and routine use, with
second level for specialised
data analysis
Applications:
The
Measurement of Fluorescence
Lifetimes not only provides the
additional parameter that is
directly related to kinetics of the
fluorophore's excited state, it also
allows separation of spectrally
indistinguishable components (e.g.
free and bound fluorophores) and the
temporal discrimination of scattered
background and background emission
(auto-fluorescence).
Fluorescein:
470nm
520nm
Application Note
Acridone:
400nm 470nm
Application Note
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Brochure