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Fluorescence Lifetime Analysis
For the advanced analysis of fluorescence and phosphorescence
decay kinetics Edinburgh Instruments provides a special software
package that meets highest standards in precision and speed of
fluorescence lifetime data recovery. The new fluorescence decay
analysis library FAST (Fluorescence Analysis Software
Technology) is extremely robust, 100% convergent, and is fully
validated with hundreds of real and simulated data. Moreover, it
is easy to use and offers a wide range of data input, on-screen
visualization, hardcopy and clipboard facilities.
The FAST software is based on proprietary and well proven
computational algorithms providing fast and reliable automatic
fitting. The proprietary algorithms combine Global Least Squares
Analysis (GLSA) minimisation with Non-negative Truncated
Singular Value Decomposition (NTSVD), and they surpasse in speed
and reliability the results the conventional Marquardt-Levenberg
algorithm.
The FAST software is particularly attractive for the analysis of
fluorescence decay kinetics that were measured with the Time
Correlated Single Photon Counting technique or by fast
multi-channel scaling and for the analysis of phosphorescence,
chemical and biological kinetics that were obtained by other
single photon counting techniques. This is because of the
Poissonian data statistics of those data that is particularly
suited for analysing complex decay processes that may extend
over a wide range of intrinsic lifetimes. FAST includes both
tail fitting using only the decay function(s) and reconvolution
fitting using decay function(s) and instrumental response
function(s).
Applications:
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Individual
multi-exponential fluorescence decay analysis
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Batch analysis of
sets of time courses
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Global
analysis of sets of decay data with wavelength dependence (TRES
data), temporal dependence, concentration dependence,
multi-titre plates, etc.
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Lifetime
distribution analysis
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Time
resolved FRET
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Time
resolved fluorescence anisotropy
Global Lifetime Data Analysis
Global Analysis is a powerful tool to simultaneously analyse a
set of multi-exponential decay curves, with a common
instrumental response function or with individual response
functions. During the simultaneous analysis one or more fit
parameters may be linked, i.e. they remain free floating, but
they are identical in all individual decay curves. The FAST
Global Analysis is exceptionally fast, even with large number of
data. The results can be easily graphically assessed and
evaluated by local and global chi-squares and local residuals.
Discrete Components Analysis
Discrete Components Analysis provides a robust multi-exponential
analysis (up to four components) with automatic selection of
initial parameter values. The numerical algorithm ensures
extremely fast and robust convergence independent of the
starting parameters. A shift between a sample time-course and an
Instrument Response Function (IRF) as well as a constant
background of both the IRF and the sample decay can be computed
automatically or fixed to predefined values.
Distribution
of Lifetime Analysis
The model free, robust and easy-to-use
Lifetime Distribution Analysis allows evaluation of lifetime
distribution functions determined on up to 200 logarithmically
or equidistantly spaced time-intervals with the possibility of
simultaneous evaluation of the shift and background parameters.
Shift and background fitting is optional. The routine is
extremely robust and fast. A priori information about
data under evaluation (a shape of the lifetime distribution) may
be introduced by using a Resolution Slider allowing regulation
of the “regularization” level. The default value of the
regularization level sets the “trade-off” between the smoothness
of the fit-function and the fitting quality.
Time Resolved Fluorescence Anisotropy
Analysis of time-resolved fluorescence anisotropy is based on
the global minimization of the parallel and perpendicular
linearly polarised fluorescence lifetime measurements with
linked parameters. A special feature is that for many
applications the G-factor comes out as a result of the data
analysis and does not need to be known prior to the analysis.
Four different analysis models are available:
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Spherical rotor
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Two spherical rotors
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Spherical rotor with restricted rotation
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Elliptical rotor
Förster Kinetics
FAST provides a model for FRET analysis of homogeneous, “rigid”
molecules (randomly distributed, slowly diffusing donor acceptor
pairs).
Additional
Functionality
FAST includes many other new features that make it unique
software for fluorescence kinetics analysis:
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Simultaneous plot of fluorescence kinetic, instrument
response function, residuals, computed graphical
representation of multi-exponential analysis and numerical
results
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Simultaneous analysis and overlapping presentation of
results for several fluorescence kinetics greatly simplifies
comparison without using any external software
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Simultaneous analysis and results presentation of
experimental data by different processing methods provides
additional confidence in the results
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Export of results in text format or in popular image formats
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Export of results into file or clipboard for easy import
into documents, presentations and image handling software
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Report generation in HTML,
“internet ready” formal for easy, operating system
independent exchange and posting on a customer web site
Download FAST
for a Trial
FAST can be downloaded free of charge for 30 days and a limited
number of fits.
Test files are
provided with the download, alternatively customer samples can
be loaded using the ASCII format that can be taken from the test
data or from the manual.
For pricing and general sales enquiries please contact:
sales@edinst.com
 Datasheet
(500kb)
 TO
DOWNLOAD A DEMO VERSION CLICK HERE
Fast
User Manual
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